Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...can also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg...290 Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives... shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing ...materials D.2 Screening for inserts E. Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol...Due to the long terminal repeats found in lentiviral plasmids, Addgene currently recommends using a strain...