...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing...scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral...

...Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol... Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection...polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for...Considerations Before You Start The health of the packaging cell line is critical for obtaining high viral...