...pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression using fluorescence...marker. Determining the titer of your lentiviral vector allows you to control the multiplicity of infection...this protocol was developed using low titer lentiviral vectors (10 5 TU/mL). If you anticipate that your...Fluorescence Titering Assay for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol ...rapidly thaw the lentiviral aliquot on ice prior to use. Prepare dilutions of the lentivirus into DMEM complete...Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker. Protocols...Introduction This protocol can be used to titer lentiviral preparations that carry a fluorescence marker...