...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store...determine what ratio gives the highest percentage of GFP positive cells. Refer to the table below for a possible...Data Figure 1: 293T cells were transfected with the GFP-expression plasmid pRosetta using μg total DNA to...efficiencies as measured by the highest proportion of GFP positive cells without limiting cell growth. Left...Left panels: bright field images; right panels: GFP channel images. Last reviewed on: August 2, 2023...

...drifted. Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into sterile tubes. Store...determine what ratio gives the highest percentage of GFP positive cells. Refer to the table below for a possible...