Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...