Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 6 results for: plasmid for e coli

Showing: 1 - 6 of 6 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...sterile water (or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin (amp...the incubation, transform DH5α E. coli cells by heatshock with the plasmid of interest. See our transformation... for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different concentrations...antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of antibiotic makes it easy...transformation protocol. Plate 50 µL of transformed E. coli /rescue media suspension onto the agar and gently...Plate with No Carbenicillin Plate shows a lawn of E. coli and no selection. 150 µL of 0.1 mg/mL Carbenicillin...Carbenicillin plated over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin...

  2. CRISPR Library Amplification

    Type
    Protocol
    ... amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....allows the amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens...differences, and plasmid recombination can all impact the representation of individual plasmids in the pooled... ). Lentiviral plasmids can recombine between their LTRs resulting in a smaller plasmid containing only...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not... tens to millions of different plasmids in a single sample. They are often used for screening, barcoding...throughput multiplexed experiments. These pooled plasmids differ by only a short region of DNA: guide RNAs...

  3. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...in healthy humans, for example, non-pathogenic E . coli . BSL-2 is for labs that work with pathogens including...here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene...

  4. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Learn how to transform E. coli with your plasmid of interest.... also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation...both storing and replicating plasmids. Because of this, nearly all plasmids (even those designed for mammalian...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific ...tube of plasmid DNA and just need to transform bacteria so that you can grow up more of the plasmid) you ...efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea to use ...introduced into a cell. Transformation of bacteria with plasmids is important not only for studies in bacteria ...

  5. General Transfection

    Type
    Protocol
    ..., the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable....therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...protocol to transfect mammalian cells with your plasmid of interest. Protocols...is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 ...

  6. Lentivirus Production

    Type
    Protocol
    ...DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert of interest..., the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...
Showing: 1 - 6 of 6 results