Lentivirus Production
Type
Protocol
...°C for several hours but should be aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as...L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) Low serum medium such as Opti-MEM...L-alanyl-L-glutamine (or stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add ...add 55 mL of heat-inactivated FBS and 5 mL of glutaGRO. Store at 4 °C. *Pro-Tip* Different brands and lots...transfection to determine what ratio gives the highest percentage of GFP positive cells. Refer to the table below...