CRISPR Library Amplification
Type
Protocol
...as these sequences are not efficiently packaged into lentiviral particles. See below for options if the...amplification. This protocol is designed to be as general as possible but note that individual libraries ...our protocol on Bacterial Transformation . The generalized pooled library amplification protocol is expected... a diagnostic digest and high-throughput next generation sequencing (NGS). Select a restriction enzyme...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...pool to 990 µL LB then perform a second and then third 1:100 dilution). Plate 100 µL of each dilution onto...recombinant plasmid likely grow faster in the closed system of a crowded plate or culture. Try restricting ...