Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...replication-incompetent lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells...Human phosphoglycerate kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene...pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials...HIV-derivative viruses. For transient knockdown of protein expression, you may transfect plasmid DNA directly into ...into the target cells. The shRNA will be expressed, but the DNA is unlikely to be integrated into the host...will allow you to select for cells that stably express your shRNA of interest. E.1 Recommended Materials...allow you to select for cells that are stably expressing your shRNA of interest. F.1. Recommended Materials...