Human Activity-Optimized CRISPR Knockout Library (2 sub-libraries in lentiCRISPRv1)
(Pooled Library #1000000067)
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Purpose
Note: This library has been replaced by an updated version (see Depositor Comments below).
The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.
The library is split into two sub-libraries, each containing 5 sgRNAs per gene.
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Vector Backbone
lentiCRISPR v1 (Plasmid #49535) - expresses Cas9
Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages users to use the updated lentiCRISPR v2 (Plasmid #52961), which can be requested separately.
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Depositing Labs
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Publication
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 1000000067 | Two gRNA pooled sub-libraries in lentiCRISPR v1 | 1 |
Library Details
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SpeciesHuman
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Genes targeted18,166
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gRNAs178,896
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Controls1,000
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Lentiviral Generation3rd
Library Shipping
Each library is delivered in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.
Pooled library #1000000067 will be delivered as two tubes, each containing a pooled sub-library. Note that each subpool must be transformed separately.
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Volume∼15 µL
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Concentration10 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: This item has been discontinued. The Sabatini and Lander labs have released an updated version of the library with an additional tube of gRNAs here.
When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71409).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Activity-Optimized Human CRISPR Pooled Library was a gift from David Sabatini and Eric Lander (Addgene #1000000067) -
For your References section:
Identification and characterization of essential genes in the human genome. Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM. Science. 2015 Oct 15. pii: aac7041. PubMed 26472758