pAct::Raf
(Plasmid #11341)

• Depositing Lab: Michael Wigler
• Publication: Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90(13):6213-7.

Backbone

• Vector backbone: pGADGH
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: byr-2
• Alt name: byr2
• Alt name: Raf homolog
• Species: S. pombe (fission yeast)
• GenBank ID: M74293
• Entrez Gene: byr2
• Tag / Fusion Protein:
  • Gal4 activation domain (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Gal4 AD

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA of fission yeast S. pombe byr-2 gene (Raf oncogene homolog) cloned into the yeast two-hybrid activation-domain vector (also called, pGADGH-byr2, Van Aelst et al., 1993, PNAS, 90(13):6213-7). pGADGH is a derivative of pGAD, which was described by Stanley Fields in PNAS Vol 88, p. 9578-9582. pGADGH contains the ADH1 promoter expressing GAL4 transcriptional activiation domain (aa768-881). The multiple cloning site of pGAD1 is replaced with the SpeI-KpnI fragment of the multiple cloning site of plasmid pBluescript SK (-).