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Igfbp1-promoter/pGL3
(Plasmid #12146)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12146 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IGFBP1 promoter
  • Alt name
    Igfbp1
  • Species
    R. norvegicus (rat)
  • Mutation
    Contains the canonical insulin response sequence (IRS) from the rat IGF binding protein-1 promoter driving a luciferase reporter
  • Entrez Gene
    Igfbp1 (a.k.a. IBP1, IGFBA, IGF-BP25)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Igfbp1-promoter/pGL3 was a gift from Domenico Accili (Addgene plasmid # 12146 ; http://n2t.net/addgene:12146 ; RRID:Addgene_12146)
  • For your References section:

    Differential regulation of gene expression by insulin and IGF-1 receptors correlates with phosphorylation of a single amino acid residue in the forkhead transcription factor FKHR. Nakae J, Barr V, Accili D. EMBO J. 2000 Mar 1. 19(5):989-96. 10.1093/emboj/19.5.989 PubMed 10698940