Addgene - pQLinkH Plasmid Data

Gene/insert name:
multi cloning site
Alt name:
pQTEV3
Insert size:
28
GenBank ID:
EF025688
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Fusion protein or tag:
attB1
Terminal:
N terminal on backbone
Fusion protein or tag:
TEV
Terminal:
N terminal on backbone
Vector backbone:
pQE-2
(Search Vector Database)
Backbone manufacturer:
Qiagen
Vector type:
Bacterial Expression
Vector type:
Co-expression
Backbone size w/o insert:
4873
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pQE65, TGAGCGGATAACAATTTCACACAG List of Sequencing Primers
3' sequencing primer:
pQE276, GGCAACCGAGCGTTCTGAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Konrad Buessow
Terms and Licenses:
MTA

Comments:

Please note that the chloramphenicol resistance gene in pQLinkH is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Vectors for co-expression of an unrestricted number of proteins. Scheich et al (Nucleic Acids Res. 2007 Feb 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13667" in your Materials and Methods section.