Addgene - pQLinkGD Plasmid Data

Plasmid 13673: pQLinkGD

Gene/insert name:
Gateway cassette
Alt name:
pDESTcoG
Insert size:
2342
GenBank ID:
EF025687
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pQE-2
(Search Vector Database)
Backbone manufacturer:
Qiagen
Vector type:
Bacterial Expression ; Co-Expression
Backbone size w/o insert (bp):
5356
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
Yes
Cloning site 3':
attR2
Site destroyed during cloning:
Yes
5' sequencing primer:
pGEX5' List of Sequencing Primers
3' sequencing primer:
pQE276, GGCAACCGAGCGTTCTGAAC
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 from Invitrogen
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Konrad Buessow
Terms and Licenses:
MTA

Comments:

Please note that the chloramphenicol resistance gene outside the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Vectors for co-expression of an unrestricted number of proteins. Scheich et al (Nucleic Acids Res. 2007 Feb 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13673" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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