Addgene - pQLinkG2 Plasmid Data

Plasmid 13671: pQLinkG2

Gene/insert name:
multi cloning site
Alt name:
pQTEV3G2
Insert size:
29
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Fusion protein or tag:
attB1
Terminal:
N terminal on backbone
Fusion protein or tag:
TEV
Terminal:
N terminal on backbone
Vector backbone:
pQE-2
(Search Vector Database)
Backbone manufacturer:
Qiagen
Vector type:
Bacterial Expression ; Co-expression
Backbone size w/o insert (bp):
5508
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX5' List of Sequencing Primers
3' sequencing primer:
pQE276, GGCAACCGAGCGTTCTGAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Konrad Buessow
Terms and Licenses:
MTA

Comments:

Please note that the chloramphenicol resistance gene in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Vectors for co-expression of an unrestricted number of proteins. Scheich et al (Nucleic Acids Res. 2007 Feb 20. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13671" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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