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ZE9 tiggywinkle hedgehog pT7TS
(Plasmid #17058)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17058 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tiggywinkle hedgehog
  • Alt name
    twhh
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    shhb (a.k.a. twh, twhh)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Steve Ekker clone A, in vector pT7TS.
Citation: Ekker et al. (1995), Current Biology 5:944-955.

T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE9 tiggywinkle hedgehog pT7TS was a gift from Randall Moon (Addgene plasmid # 17058)