ZE9 tiggywinkle hedgehog pT7TS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17058||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeZebrafish Expression
Growth in Bacteria
Gene/Insert nametiggywinkle hedgehog
SpeciesD. rerio (zebrafish)
Entrez Geneshhb (a.k.a. twh, twhh)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Steve Ekker clone A, in vector pT7TS.
Citation: Ekker et al. (1995), Current Biology 5:944-955.
T7TS-twhh was made by insertion of twhh open-reading frame into the SpeI site of the pT7TS vector. This vector adds Xenopus laevis β-globin 5′ and 3′ untranslated sequences to the inserted open-reading frame for increased transcript stability. The ORF was made by PCR using the following primers and appropriate cDNA as template:(5′-GGACTAGTCACCATGGA-CGTAAGGCTGCA-3′), and (5′-AGCATACTAGTA-GTCTCAACTTAAGTGTAA-3′).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE9 tiggywinkle hedgehog pT7TS was a gift from Randall Moon (Addgene plasmid # 17058 ; http://n2t.net/addgene:17058 ; RRID:Addgene_17058)