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1352wUV2 F2bbs kan GCANNNAGGk
(Plasmid #18752)


Item Catalog # Description Quantity Price (USD)
Plasmid 18752 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    3 tandem zinc fingers
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    A Kan cassette (1090bp) is inserted between two bbsI sites in finger 2 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 2 libraries. Fingers one and three are anchor fingers which specify AGG and GCA, respectively.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer OMG5
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1352wUV2 F2bbs kan GCANNNAGGk was a gift from Scot Wolfe (Addgene plasmid # 18752)
  • For your References section:

    Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337