A Kan cassette (1090bp) is inserted between two bbsI sites in finger 2 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 2 libraries. Fingers one and three are anchor fingers which specify AGG and GCA, respectively.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
18752" in your Materials and Methods section.