|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18754||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4760
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namezinc finger nuclease
SpeciesM. musculus (mouse)
Insert Size (bp)267
MutationRNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the RR version of the heterodimeric set. In addition there is an N-terminal HA tag.
- Cloning method Restriction Enzyme
- 5′ cloning site kpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CS2 Forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-HA-GAAZFP-FokI-RR was a gift from Scot Wolfe (Addgene plasmid # 18754)
For your References section:Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337