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pCS2-HA-GAAZFP-FokI-RR
(Plasmid #18754)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    zinc finger nuclease
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    267
  • Mutation
    RNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the RR version of the heterodimeric set. In addition there is an N-terminal HA tag.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CS2 Forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-HA-GAAZFP-FokI-RR was a gift from Scot Wolfe (Addgene plasmid # 18754)
  • For your References section:

    Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337