RNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the DD version of the heterodimeric set. In addition there is an N-terminal flag tag.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
18755" in your Materials and Methods section.