Addgene - pCS2-Flag-TTGZFP-FokI-DD Plasmid Data

Plasmid 18755: pCS2-Flag-TTGZFP-FokI-DD

Gene/insert name:
zinc finger nuclease
Insert size:
267
Species:
M. musculus (mouse)
Mutation:
RNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the DD version of the heterodimeric set. In addition there is an N-terminal flag tag.
Vector backbone:
CS2
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4760
Cloning site 5':
kpnI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CS2 forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng et al (Nat Biotechnol. 2008 Jun . 26(6):695-701. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18755" in your Materials and Methods section.