1
Plasmid 18755: pCS2-Flag-TTGZFP-FokI-DD
  • zinc finger nuclease

  • 267

  • M. musculus (mouse)

  • RNA expression vector for expressing the zinc finger nuclease. Zinc fingers are subcloned between the kpn1 and bamHI sites. This results in a direct, in frame fusion with the C-terminal FokI nuclease. In this case the nuclease is the DD version of the heterodimeric set. In addition there is an N-terminal flag tag.

  • CS2
    (Search Vector Database)

  • Bacterial Expression

  • 4760

  • kpnI

  • No

  • BamHI

  • No

  • CS2 forward List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Scot Wolfe

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng et al (Nat Biotechnol. 2008 Jun . 26(6):695-701. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18755" in your Materials and Methods section.