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Plasmid 23500: pDONR223-PRKD1
  • PRKD1

  • protein kinase D1

  • 2739

  • H. sapiens (human)

  • PRKD1 (PKC-MU, PKCM, PKD, PRKCM)

  • 1MM

  • pDONR223
    (Search Vector Database)

  • Invitrogen

  • Gateway Donor vector

  • 5005

  • Gateway Cloning

  • M13pUC-fwd List of Sequencing Primers

  • M13rev

  • Spectinomycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • William Hahn

  • David Root

  • UBMTA

Comments: 

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.

A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen et al (Nature. 2010 Nov 24. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23500" in your Materials and Methods section.