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Human Kinase ORF Kit
(Kit # 1000000014 )

Depositing Labs:   William Hahn, David Root

This kit consists of 558 distinct human kinases and kinase-related protein Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity.

This kit consists of six 96-well plates, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$1500 USD + shipping
Available to Academic and Nonprofits Only

Original Publications

These plasmids have been described in:

A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 26;8(8):659-61, DOI: 10.1038/nmeth.1638 Publication Date: 2011 June. - PubMed.

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA. Nature 16;468(7326):968-72 DOI: 10.1038/nature09627 Publication Date: 2010 Dec. - PubMed.

Description

The Center for Cancer Systems Biology (Dana Farber Cancer Institute)-Broad Human Kinase ORF collection from Addgene consists of 559 distinct human kinases and kinase-related protein ORFs in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity. For more information see the associated publications.

Kinase ORFs were assembled from a number of sources; 56% were isolated as single cloned isolates from the ORFeome 5.1 collection (http://horfdb.dfci.harvard.edu); 31% were cloned from normal human tissue RNA (Ambion) by reverse transcription and subsequent PCR amplification adding Gateway® sequences; 11% were cloned into Entry vectors from templates provided by the Harvard Institute of Proteomics (HIP); 2% additional kinases were cloned into Entry vectors from templates obtained from collaborating laboratories. All ORFs are open (stop codons removed) except for 5 (MST1R, PTK7, JAK3, AXL, TIE1) which are closed (have stop codons).

human-kinase-pie.jpg

Read the interview with DFCI Scientists, William Hahn and Jesse Boehm, who created the kinase libary. Click Here

Kit Documentation

Icon Broad human Kinase Kit info pages.pdf (186.7 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit was a gift from William Hahn and David Root (Addgene kit # 1000000014)"

For your Reference section:
A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 26;8(8):659-61, DOI: 10.1038/nmeth.1638 Publication Date: 2011 June. - PubMed.

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA.Nature 16;468(7326):968-72 DOI: 10.1038/nature09627 Publication Date: 2010 Dec. - PubMed.

Resistance Color Key:

 
  Spectinomycin  

Inventory

Well Plasmid Resistance
1 / A / 1 pDONR223-CAMK2B
  Spectinomycin
1 / A / 2 pDONR223-NEK3
  Spectinomycin
1 / A / 3 pDONR223-ACVR1
  Spectinomycin
1 / A / 4 pDONR223-NME1-NME2
  Spectinomycin
1 / A / 5 pDONR223-CRKL
  Spectinomycin
1 / A / 6 pDONR223-CSNK1A1
  Spectinomycin
1 / A / 7 pDONR223-CCL4
  Spectinomycin
1 / A / 8 pDONR223-STK33
  Spectinomycin
1 / A / 9 pDONR223-C1orf57
  Spectinomycin
1 / A / 10 pDONR223-CSNK2B
  Spectinomycin
1 / A / 11 pDONR223-PRKAB1
  Spectinomycin
1 / A / 12 pDONR223-LOC389599
  Spectinomycin
1 / B / 1 pDONR223-CAMKK1
  Spectinomycin
1 / B / 2 pDONR223-DYRK4
  Spectinomycin
1 / B / 3 pDONR223-CIB1
  Spectinomycin
1 / B / 4 pDONR223-GK2
  Spectinomycin
1 / B / 5 pDONR223-PRKCI
  Spectinomycin
1 / B / 6 pDONR223-FLJ25006
  Spectinomycin
1 / B / 7 pDONR223-CDK3
  Spectinomycin
1 / B / 8 pDONR223-PION
  Spectinomycin
1 / B / 9 pDONR223-STK19
  Spectinomycin
1 / B / 10 pDONR223-GRK6
  Spectinomycin
1 / B / 11 pDONR223-LOC442075
  Spectinomycin
1 / B / 12 pDONR223-LOC390877
  Spectinomycin
1 / C / 1 pDONR223-AURKC
  Spectinomycin
1 / C / 2 pDONR223-FLJ23356
  Spectinomycin
1 / C / 3 pDONR223-PRKAR1B
  Spectinomycin
1 / C / 4 pDONR223-FLJ40852
  Spectinomycin
1 / C / 5 pDONR223-SGK2
  Spectinomycin
1 / C / 6 pDONR223-TESK1
  Spectinomycin
1 / C / 7 pDONR223-GUK1
  Spectinomycin
1 / C / 8 pDONR223-DCK
  Spectinomycin
1 / C / 9 pDONR223-CDKL4
  Spectinomycin
1 / C / 10 pDONR223-PRKX
  Spectinomycin
1 / C / 11 pDONR223-PANK3
  Spectinomycin
1 / C / 12 pDONR223-PHKG1
  Spectinomycin
1 / D / 1 pDONR223-CIB4
  Spectinomycin
1 / D / 2 pDONR223-PKDCC
  Spectinomycin
1 / D / 3 pDONR223-NUAK2
  Spectinomycin
1 / D / 4 pDONR223-CDKL1
  Spectinomycin
1 / D / 5 pDONR223-DAPK2
  Spectinomycin
1 / D / 6 pDONR223-LOC649288
  Spectinomycin
1 / D / 7 pDONR223-NME2
  Spectinomycin
1 / D / 8 pDONR223-ITGB1BP3
  Spectinomycin
1 / D / 9 pDONR223-LOC652799
  Spectinomycin
1 / D / 10 pDONR223-NEK7
  Spectinomycin
1 / D / 11 pDONR223-SLAMF6
  Spectinomycin
1 / D / 12 pDONR223-TSSK2
  Spectinomycin
1 / E / 1 pDONR223-RAF1
  Spectinomycin
1 / E / 2 pDONR223-BMP2KL
  Spectinomycin
1 / E / 3 pDONR223-CSNK1G3
  Spectinomycin
1 / E / 4 pDONR223-ACVR2B
  Spectinomycin
1 / E / 5 pDONR223-GRK7
  Spectinomycin
1 / E / 6 pDONR223-PAK6
  Spectinomycin
1 / E / 7 pDONR223-MARK2
  Spectinomycin
1 / E / 8 pDONR223-SNRK
  Spectinomycin
1 / E / 9 pDONR223-MAP2K1
  Spectinomycin
1 / E / 10 pDONR223-PLXNB2
  Spectinomycin
1 / E / 11 pDONR223-CAMK2A
  Spectinomycin
1 / E / 12 pDONR223-CAMK2G
  Spectinomycin
1 / F / 1 pDONR223-BRD3
  Spectinomycin
1 / F / 2 pDONR223-MPP7
  Spectinomycin
1 / F / 3 pDONR223-IRAK2
  Spectinomycin
1 / F / 4 pDONR223-PRKG1
  Spectinomycin
1 / F / 5 pDONR223-PFKFB1
  Spectinomycin
1 / F / 6 pDONR223-IPPK
  Spectinomycin
1 / F / 7 pDONR223-MPP6
  Spectinomycin
1 / F / 8 pDONR223-MPP4
  Spectinomycin
1 / F / 9 pDONR223-NEK8
  Spectinomycin
1 / F / 10 pDONR223-PANK4
  Spectinomycin
1 / F / 11 pDONR223-MAPK8
  Spectinomycin
1 / F / 12 pDONR223-CDKL2
  Spectinomycin
1 / G / 1 pDONR223-CAMKK2
  Spectinomycin
1 / G / 2 pDONR223-BMP2K
  Spectinomycin
1 / G / 3 pDONR223-PRKCQ
  Spectinomycin
1 / G / 4 pDONR223-LIMK2
  Spectinomycin
1 / G / 5 pDONR223-MAPKAPK2
  Spectinomycin
1 / G / 6 pDONR223-PGK1
  Spectinomycin
1 / G / 7 pDONR223-CHKA
  Spectinomycin
1 / G / 8 pDONR223-CDC2L6
  Spectinomycin
1 / G / 9 pDONR223-ADRBK1
  Spectinomycin
1 / G / 10 pDONR223-MPP3
  Spectinomycin
1 / G / 11 pDONR223-BRSK2
  Spectinomycin
1 / G / 12 pDONR223-UHMK1
  Spectinomycin
1 / H / 1 pDONR223-NEK5
  Spectinomycin
1 / H / 2 pDONR223-PRKG2
  Spectinomycin
1 / H / 3 pDONR223-DAPK3
  Spectinomycin
1 / H / 4 pDONR223-NEK10
  Spectinomycin
1 / H / 5 pDONR223-TGFBR1
  Spectinomycin
1 / H / 6 pDONR223-PAK3
  Spectinomycin
1 / H / 7 pDONR223-SRPK3
  Spectinomycin
1 / H / 8 pDONR223-MAPK4
  Spectinomycin
1 / H / 9 pDONR223-RIPK1
  Spectinomycin
1 / H / 10 pDONR223-KSR
  Spectinomycin
1 / H / 11 pDONR223-MAPK6
  Spectinomycin
1 / H / 12 pDONR223-DGKA
  Spectinomycin
2 / A / 1 pDONR223-SNF1LK
  Spectinomycin
2 / A / 2 pDONR223-GTF2H1
  Spectinomycin
2 / A / 3 pDONR223-RKHD3
  Spectinomycin
2 / A / 4 pDONR223-MPP5
  Spectinomycin
2 / A / 5 pDONR223-GNE
  Spectinomycin
2 / A / 6 pDONR223-IHPK3
  Spectinomycin
2 / A / 7 pDONR223-PIP5K2C
  Spectinomycin
2 / A / 8 pDONR223-DLG3
  Spectinomycin
2 / A / 9 pDONR223-PRKR
  Spectinomycin
2 / A / 10 pDONR223-AMHR2
  Spectinomycin
2 / A / 11 pDONR223-MAP3K2
  Spectinomycin
2 / A / 12 pDONR223-BRD4
  Spectinomycin
2 / B / 1 pDONR223-MAST2
  Spectinomycin
2 / B / 2 pDONR223-PIK3C3
  Spectinomycin
2 / B / 3 pDONR223-SCYL2
  Spectinomycin
2 / B / 4 pDONR223-NEK9
  Spectinomycin
2 / B / 5 pDONR223-RPS6KC1
  Spectinomycin
2 / B / 6 pDONR223-PIK4CB
  Spectinomycin
2 / B / 7 pDONR223-EIF2AK4
  Spectinomycin
2 / B / 8 pDONR223-CKS2
  Spectinomycin
2 / B / 9 pDONR223-RPS6KL1
  Spectinomycin
2 / B / 10 pDONR223-PRPF4B
  Spectinomycin
2 / B / 11 pDONR223-PIK3CA
  Spectinomycin
2 / B / 12 pDONR223-HIPK3
  Spectinomycin
2 / C / 1 pDONR223-ALDH18A1
  Spectinomycin
2 / C / 2 pDONR223-TNNI3K
  Spectinomycin
2 / C / 3 pDONR223-CASK
  Spectinomycin
2 / C / 4 pDONR223-PHKA1
  Spectinomycin
2 / C / 5 pDONR223-GSG2
  Spectinomycin
2 / C / 6 pDONR223-MAP3K11
  Spectinomycin
2 / C / 7 pDONR223-ULK2
  Spectinomycin
2 / C / 8 pDONR223-DKFZp761P0423
  Spectinomycin
2 / C / 9 pDONR223-TRPM7
  Spectinomycin
2 / C / 10 pDONR223-DGKB
  Spectinomycin
2 / C / 11 pDONR223-TGFBR3
  Spectinomycin
2 / C / 12 pDONR223-NPR2
  Spectinomycin
2 / D / 1 pDONR223-DAPK1
  Spectinomycin
2 / D / 2 pDONR223-HIPK2
  Spectinomycin
2 / D / 3 pDONR223-CARD11
  Spectinomycin
2 / D / 4 pDONR223-PIK3C2G
  Spectinomycin
2 / D / 5 pDONR223-MAP4K1
  Spectinomycin
2 / D / 6 pDONR223-LATS2
  Spectinomycin
2 / D / 7 pDONR223-MAP4K4
  Spectinomycin
2 / D / 8 pDONR223-DGKK
  Spectinomycin
2 / D / 9 pDONR223-PIK3R4
  Spectinomycin
2 / D / 10 pDONR223-LRGUK
  Spectinomycin
2 / D / 11 pDONR223-PRKD2
  Spectinomycin
2 / D / 12 pDONR223-ERN1
  Spectinomycin
2 / E / 1 pDONR223-KIAA2002
  Spectinomycin
2 / E / 2 pDONR223-ALPK2
  Spectinomycin
2 / E / 3 pDONR223-BRAF
  Spectinomycin
2 / E / 4 pDONR223-PRKACA
  Spectinomycin
2 / E / 5 pDONR223-VRK1
  Spectinomycin
2 / E / 6 pDONR223-NEK4
  Spectinomycin
2 / E / 7 pDONR223-MAPK1
  Spectinomycin
2 / E / 8 pDONR223-MAP3K15
  Spectinomycin
2 / E / 9 pDONR223-PRKD1
  Spectinomycin
2 / E / 10 pDONR223-KSR2
  Spectinomycin
2 / E / 11 pDONR223-CHEK2
  Spectinomycin
2 / E / 12 pDONR223-PI4KII
  Spectinomycin
2 / F / 1 pDONR223-PINK1
  Spectinomycin
2 / F / 2 pDONR223-DLG1
  Spectinomycin
2 / F / 3 pDONR223-PRKY
  Spectinomycin
2 / F / 4 pDONR223-PI4K2B
  Spectinomycin
2 / F / 5 pDONR223-DKFZp434B1231
  Spectinomycin
2 / F / 6 pDONR223-MAPK3
  Spectinomycin
2 / F / 7 pDONR223-GSK3A
  Spectinomycin
2 / F / 8 pDONR223-LIMK1
  Spectinomycin
2 / F / 9 pDONR223-AAK1
  Spectinomycin
2 / F / 10 pDONR223-LOC648152
  Spectinomycin
2 / F / 11 pDONR223-PSKH1
  Spectinomycin
2 / F / 12 pDONR223-MASTL
  Spectinomycin
2 / G / 1 pDONR223-PCTK2
  Spectinomycin
2 / G / 2 pDONR223-MAP3K5
  Spectinomycin
2 / G / 3 pDONR223-PLXNA3
  Spectinomycin
2 / G / 4 pDONR223-TPR
  Spectinomycin
2 / G / 5 pDONR223-LRRK2
  Spectinomycin
2 / G / 6 pDONR223-LRPPRC
  Spectinomycin
2 / G / 7 pDONR223-MINK1
  Spectinomycin
2 / G / 8 pDONR223-MAGI1
  Spectinomycin
2 / G / 9 pDONR223-MYO3B
  Spectinomycin
2 / G / 10 pDONR223-CDC42BPG
  Spectinomycin
2 / G / 11 pDONR223-PHKA2
  Spectinomycin
2 / G / 12 pDONR223-RPS6KA3
  Spectinomycin
2 / H / 1 pDONR223-RIPK2
  Spectinomycin
2 / H / 2 pDONR223-DGKG
  Spectinomycin
2 / H / 3 pDONR223-RPS6KA2
  Spectinomycin
2 / H / 4 pDONR223-MKNK1
  Spectinomycin
2 / H / 5 pDONR223-AURKA
  Spectinomycin
2 / H / 6 pDONR223-RPS6KB2
  Spectinomycin
2 / H / 7 pDONR223-VRK2
  Spectinomycin
2 / H / 8 pDONR223-RIOK2
  Spectinomycin
2 / H / 9 pDONR223-IKBKE
  Spectinomycin
2 / H / 10 pDONR223-RIOK3
  Spectinomycin
2 / H / 11 pDONR223-OXSR1
  Spectinomycin
2 / H / 12 pDONR223-PIP5K1B
  Spectinomycin
3 / A / 1 pDONR223-MAP3K8
  Spectinomycin
3 / A / 2 pDONR223-AKT3
  Spectinomycin
3 / A / 3 pDONR223-RPS6KA6
  Spectinomycin
3 / A / 4 pDONR223-PIK4CA
  Spectinomycin
3 / A / 5 pDONR223-PIK3CB
  Spectinomycin
3 / A / 6 pDONR223-PAK1
  Spectinomycin
3 / A / 7 pDONR223-CAMK1
  Spectinomycin
3 / A / 8 pDONR223-LOC340371
  Spectinomycin
3 / A / 9 pDONR223-LOC91807
  Spectinomycin
3 / A / 10 pDONR223-MORN2
  Spectinomycin
3 / A / 11 pDONR223-ULK4
  Spectinomycin
3 / A / 12 pDONR223-PRKAG3
  Spectinomycin
3 / B / 1 pDONR223-PRKCA
  Spectinomycin
3 / B / 2 pDONR223-PRKCE
  Spectinomycin
3 / B / 3 pDONR223-CCL2
  Spectinomycin
3 / B / 4 pDONR223-TSSK3
  Spectinomycin
3 / B / 5 pDONR223-GK5
  Spectinomycin
3 / B / 6 pDONR223-MAP2K2
  Spectinomycin
3 / B / 7 pDONR223-PDK3
  Spectinomycin
3 / B / 8 pDONR223-FLJ10986
  Spectinomycin
3 / B / 9 pDONR223-IHPK1
  Spectinomycin
3 / B / 10 pDONR223-NUP62
  Spectinomycin
3 / B / 11 pDONR223-FASTKD5
  Spectinomycin
3 / B / 12 pDONR223-RP6-213H19.1
  Spectinomycin
3 / C / 1 pDONR223-NME6
  Spectinomycin
3 / C / 2 pDONR223-DTYMK
  Spectinomycin
3 / C / 3 pDONR223-AK3L1
  Spectinomycin
3 / C / 4 pDONR223-STK16
  Spectinomycin
3 / C / 5 pDONR223-SH3BP5L
  Spectinomycin
3 / C / 6 pDONR223-ACVR1B
  Spectinomycin
3 / C / 7 pDONR223-NRBP
  Spectinomycin
3 / C / 8 pDONR223-COL4A3BP
  Spectinomycin
3 / C / 9 pDONR223-PAK7
  Spectinomycin
3 / C / 10 pDONR223-ASCIZ
  Spectinomycin
3 / C / 11 pDONR223-PNCK
  Spectinomycin
3 / C / 12 pDONR223-NME1
  Spectinomycin
3 / D / 1 pDONR223-NME3
  Spectinomycin
3 / D / 2 pDONR223-AK1
  Spectinomycin
3 / D / 3 pDONR223-TBK1
  Spectinomycin
3 / D / 4 pDONR223-PDK4
  Spectinomycin
3 / D / 5 pDONR223-AGK
  Spectinomycin
3 / D / 6 pDONR223-SH3BP5
  Spectinomycin
3 / D / 7 pDONR223-PRKD3
  Spectinomycin
3 / D / 8 pDONR223-PAPSS2
  Spectinomycin
3 / D / 9 pDONR223-SRPK1
  Spectinomycin
3 / D / 10 pDONR223-PANK2
  Spectinomycin
3 / D / 11 pDONR223-NME4
  Spectinomycin
3 / D / 12 pDONR223-DCAKD
  Spectinomycin
3 / E / 1 pDONR223-KHK
  Spectinomycin
3 / E / 2 pDONR223-PIM2
  Spectinomycin
3 / E / 3 pDONR223-PRPS1
  Spectinomycin
3 / E / 4 pDONR223-TRIB2
  Spectinomycin
3 / E / 5 pDONR223-CD2
  Spectinomycin
3 / E / 6 pDONR223-PDGFRL
  Spectinomycin
3 / E / 7 pDONR223-KIAA0999
  Spectinomycin
3 / E / 8 pDONR223-HIPK1
  Spectinomycin
3 / E / 9 pDONR223-MAP3K6
  Spectinomycin
3 / E / 10 pDONR223-STK24
  Spectinomycin
3 / E / 11 pDONR223-MGC42105
  Spectinomycin
3 / E / 12 pDONR223-BRSK1
  Spectinomycin
3 / F / 1 pDONR223-C9orf98
  Spectinomycin
3 / F / 2 pDONR223-SGK3
  Spectinomycin
3 / F / 3 pDONR223-FGFRL1
  Spectinomycin
3 / F / 4 pDONR223-PI4KAP2
  Spectinomycin
3 / F / 5 pDONR223-TXNDC3
  Spectinomycin
3 / F / 6 pDONR223-FASTKD1
  Spectinomycin
3 / F / 7 pDONR223-DCAMKL2
  Spectinomycin
3 / F / 8 pDONR223-FASTKD2
  Spectinomycin
3 / F / 9 pDONR223-HSPB8
  Spectinomycin
3 / F / 10 pDONR223-TRIM27
  Spectinomycin
3 / F / 11 pDONR223-PLXNA4B
  Spectinomycin
3 / F / 12 pDONR223-CABC1
  Spectinomycin
3 / G / 1 pDONR223-MGC16169
  Spectinomycin
3 / G / 2 pDONR223-MAP4K5
  Spectinomycin
3 / G / 3 pDONR223-BUB1
  Spectinomycin
3 / G / 4 pDONR223-HKDC1
  Spectinomycin
3 / G / 5 pDONR223-SCYL1
  Spectinomycin
3 / G / 6 pDONR223-ACVR1C
  Spectinomycin
3 / G / 7 pDONR223-YSK4
  Spectinomycin
3 / G / 8 pDONR223-SNX16
  Spectinomycin
3 / G / 9 pDONR223-BMPR1A
  Spectinomycin
3 / G / 10 pDONR223-ARSG
  Spectinomycin
3 / G / 11 pDONR223-FXN
  Spectinomycin
3 / G / 12 pDONR223-FN3K
  Spectinomycin
3 / H / 1 pDONR223-PRPS2
  Spectinomycin
3 / H / 2 pDONR223-TGFBR2
  Spectinomycin
3 / H / 3 pDONR223-MAK
  Spectinomycin
3 / H / 4 pDONR223-PGK2
  Spectinomycin
3 / H / 5 pDONR223-MAP3K12
  Spectinomycin
3 / H / 6 pDONR223-IRAK3
  Spectinomycin
3 / H / 7 pDONR223-CDKL3
  Spectinomycin
3 / H / 8 pDONR223-TLK2
  Spectinomycin
3 / H / 9 pDONR223-ALPK1
  Spectinomycin
3 / H / 10 pDONR223-PIP5KL1
  Spectinomycin
3 / H / 11 pDONR223-HK2
  Spectinomycin
3 / H / 12 pDONR223-MAST1
  Spectinomycin
4 / A / 1 pDONR223-ALS2CR7
  Spectinomycin
4 / A / 2 pDONR223-LYK5
  Spectinomycin
4 / A / 3 pDONR223-ETNK1
  Spectinomycin
4 / A / 4 pDONR223-DYRK3
  Spectinomycin
4 / A / 5 pDONR223-RP2
  Spectinomycin
4 / A / 6 pDONR223-MAPKAPK5
  Spectinomycin
4 / A / 7 pDONR223-BMPR1B
  Spectinomycin
4 / A / 8 pDONR223-XYLB
  Spectinomycin
4 / A / 9 pDONR223-PRKCG
  Spectinomycin
4 / A / 10 pDONR223-RPS6KA4
  Spectinomycin
4 / A / 11 pDONR223-NLK
  Spectinomycin
4 / A / 12 pDONR223-SH3BP4
  Spectinomycin
4 / B / 1 pDONR223-MAP4K2
  Spectinomycin
4 / B / 2 pDONR223-RAGE
  Spectinomycin
4 / B / 3 pDONR223-ULK3
  Spectinomycin
4 / B / 4 pDONR223-PRKAB2
  Spectinomycin
4 / B / 5 pDONR223-NJMU-R1
  Spectinomycin
4 / B / 6 pDONR223-MAPK10
  Spectinomycin
4 / B / 7 pDONR223-ADCK1
  Spectinomycin
4 / B / 8 pDONR223-PTK9
  Spectinomycin
4 / B / 9 pDONR223-CDK10
  Spectinomycin
4 / B / 10 pDONR223-GLYCTK
  Spectinomycin
4 / B / 11 pDONR223-ACVR2A
  Spectinomycin
4 / B / 12 pDONR223-PAK2
  Spectinomycin
4 / C / 1 pDONR223-MYLK2
  Spectinomycin
4 / C / 2 pDONR223-MOS
  Spectinomycin
4 / C / 3 pDONR223-NEK2
  Spectinomycin
4 / C / 4 pDONR223-TPK1
  Spectinomycin
4 / C / 5 pDONR223-COASY
  Spectinomycin
4 / C / 6 pDONR223-PIK3CG
  Spectinomycin
4 / C / 7 pDONR223-RIPK5
  Spectinomycin
4 / C / 8 pDONR223-TTK
  Spectinomycin
4 / C / 9 pDONR223-MAP4K3
  Spectinomycin
4 / C / 10 pDONR223-LATS1
  Spectinomycin
4 / C / 11 pDONR223-IPMK
  Spectinomycin
4 / C / 12 pDONR223-PRKAR2B
  Spectinomycin
4 / D / 1 pDONR223-PFKFB3
  Spectinomycin
4 / D / 2 pDONR223-BMPR2
  Spectinomycin
4 / D / 3 pDONR223-WNK1
  Spectinomycin
4 / D / 4 pDONR223-PRKAA2
  Spectinomycin
4 / D / 5 pDONR223-PKLR
  Spectinomycin
4 / D / 6 pDONR223-MPP2
  Spectinomycin
4 / D / 7 pDONR223-EXOSC10
  Spectinomycin
4 / D / 8 pDONR223-XRCC6BP1
  Spectinomycin
4 / D / 9 pDONR223-AK2
  Spectinomycin
4 / D / 10 pDONR223-TRIB3
  Spectinomycin
4 / D / 11 pDONR223-CSNK2A1
  Spectinomycin
4 / D / 12 pDONR223-FN3KRP
  Spectinomycin
4 / E / 1 pDONR223-SCYL3
  Spectinomycin
4 / E / 2 pDONR223-RBKS
  Spectinomycin
4 / E / 3 pDONR223-AURKB
  Spectinomycin
4 / E / 4 pDONR223-BUB1B
  Spectinomycin
4 / E / 5 pDONR223-FUK
  Spectinomycin
4 / E / 6 pDONR223-CLK2
  Spectinomycin
4 / E / 7 pDONR223-RPS6KB1
  Spectinomycin
4 / E / 8 pDONR223-VRK3
  Spectinomycin
4 / E / 9 pDONR223-CDK6
  Spectinomycin
4 / E / 10 pDONR223-PRKAG2
  Spectinomycin
4 / E / 11 pDONR223-DYRK2
  Spectinomycin
4 / E / 12 pDONR223-PLK2
  Spectinomycin
4 / F / 1 pDONR223-PIM1
  Spectinomycin
4 / F / 2 pDONR223-MAP3K7
  Spectinomycin
4 / F / 3 pDONR223-MAP2K1IP1
  Spectinomycin
4 / F / 4 pDONR223-TESK2
  Spectinomycin
4 / F / 5 pDONR223-PIK3R1
  Spectinomycin
4 / F / 6 pDONR223-MAPK15
  Spectinomycin
4 / F / 7 pDONR223-RFK
  Spectinomycin
4 / F / 8 pDONR223-CDK5
  Spectinomycin
4 / F / 9 pDONR223-TWF2
  Spectinomycin
4 / F / 10 pDONR223-CDK9
  Spectinomycin
4 / F / 11 pDONR223-NME7
  Spectinomycin
4 / F / 12 pDONR223-MVK
  Spectinomycin
4 / G / 1 pDONR223-SPHK1
  Spectinomycin
4 / G / 2 pDONR223-CSNK1G2
  Spectinomycin
4 / G / 3 pDONR223-CKMT1A
  Spectinomycin
4 / G / 4 pDONR223-PHKB
  Spectinomycin
4 / G / 5 pDONR223-SGK
  Spectinomycin
4 / G / 6 pDONR223-ZAK
  Spectinomycin
4 / G / 7 pDONR223-PCTK3
  Spectinomycin
4 / G / 8 pDONR223-ADCK4
  Spectinomycin
4 / G / 9 pDONR223-FASTK
  Spectinomycin
4 / G / 10 pDONR223-PAK4
  Spectinomycin
4 / G / 11 pDONR223-SPHK2
  Spectinomycin
4 / G / 12 pDONR223-PCK2
  Spectinomycin
4 / H / 1 pDONR223-MARK3
  Spectinomycin
4 / H / 2 pDONR223-CCRK
  Spectinomycin
4 / H / 3 pDONR223-PRKAG1
  Spectinomycin
4 / H / 4 pDONR223-MAPK12
  Spectinomycin
4 / H / 5 pDONR223-STK32C
  Spectinomycin
4 / H / 6 pDONR223-STK17B
  Spectinomycin
4 / H / 7 pDONR223-ADCK2
  Spectinomycin
4 / H / 8 pDONR223-PXK
  Spectinomycin
4 / H / 9 pDONR223-PRKCZ
  Spectinomycin
4 / H / 10 pDONR223-ARAF
  Spectinomycin
4 / H / 11 pDONR223-RPS6KA1
  Spectinomycin
4 / H / 12 pDONR223-TAOK3
  Spectinomycin
5 / A / 1 pDONR223-EEF2K
  Spectinomycin
5 / A / 2 pDONR223-TLK1
  Spectinomycin
5 / A / 3 pDONR223-PFKM
  Spectinomycin
5 / A / 4 pDONR223-PFKL
  Spectinomycin
5 / A / 5 pDONR223-HK1
  Spectinomycin
5 / A / 6 pDONR223-HK3
  Spectinomycin
5 / A / 7 pDONR223-MAP3K14
  Spectinomycin
5 / A / 8 pDONR223-NYD-SP25
  Spectinomycin
5 / A / 9 pDONR223-CALM2
  Spectinomycin
5 / A / 10 pDONR223-MKNK2
  Spectinomycin
5 / A / 11 pDONR223-TK1
  Spectinomycin
5 / A / 12 pDONR223-CSNK1G1
  Spectinomycin
5 / B / 1 pDONR223-PDIK1L
  Spectinomycin
5 / B / 2 pDONR223-MAPK13
  Spectinomycin
5 / B / 3 pDONR223-TSSK1
  Spectinomycin
5 / B / 4 pDONR223-PRKAR1A
  Spectinomycin
5 / B / 5 pDONR223-CKB
  Spectinomycin
5 / B / 6 pDONR223-GALK1
  Spectinomycin
5 / B / 7 pDONR223-PIP5K2A
  Spectinomycin
5 / B / 8 pDONR223-PHKG2
  Spectinomycin
5 / B / 9 pDONR223-CKMT2
  Spectinomycin
5 / B / 10 pDONR223-NADK
  Spectinomycin
5 / B / 11 pDONR223-GALK2
  Spectinomycin
5 / B / 12 pDONR223-IRAK4
  Spectinomycin
5 / C / 1 pDONR223-GCK
  Spectinomycin
5 / C / 2 pDONR223-MPP1
  Spectinomycin
5 / C / 3 pDONR223-AKT1
  Spectinomycin
5 / C / 4 pDONR223-CLK3
  Spectinomycin
5 / C / 5 pDONR223-PCTK1
  Spectinomycin
5 / C / 6 pDONR223-PIP5K1A
  Spectinomycin
5 / C / 7 pDONR223-CAMKV
  Spectinomycin
5 / C / 8 pDONR223-PKM2
  Spectinomycin
5 / C / 9 pDONR223-RPS6KA5
  Spectinomycin
5 / C / 10 pDONR223-RIOK1
  Spectinomycin
5 / C / 11 pDONR223-HIPK4
  Spectinomycin
5 / C / 12 pDONR223-DYRK1B
  Spectinomycin
5 / D / 1 pDONR223-ITPKB
  Spectinomycin
5 / D / 2 pDONR223-AK7
  Spectinomycin
5 / D / 3 pDONR223-PRKCB2
  Spectinomycin
5 / D / 4 pDONR223-PRKCH
  Spectinomycin
5 / D / 5 pDONR223-SRPK2
  Spectinomycin
5 / D / 6 pDONR223-CKS1B
  Spectinomycin
5 / D / 7 pDONR223-CALM1
  Spectinomycin
5 / D / 8 pDONR223-CALM3
  Spectinomycin
5 / D / 9 pDONR223-STK32A
  Spectinomycin
5 / D / 10 pDONR223-PMVK
  Spectinomycin
5 / D / 11 pDONR223-NME5
  Spectinomycin
5 / D / 12 pDONR223-AK3
  Spectinomycin
5 / E / 1 pDONR223-TP53RK
  Spectinomycin
5 / E / 2 pDONR223-TSSK6
  Spectinomycin
5 / E / 3 pDONR223-CDC2
  Spectinomycin
5 / E / 4 pDONR223-CDK2
  Spectinomycin
5 / E / 5 pDONR223-CDK4
  Spectinomycin
5 / E / 6 pDONR223-CDK5R1
  Spectinomycin
5 / E / 7 pDONR223-PDXK
  Spectinomycin
5 / E / 8 pDONR223-NEK6
  Spectinomycin
5 / E / 9 pDONR223-PBK
  Spectinomycin
5 / E / 10 pDONR223-MAP2K6
  Spectinomycin
5 / E / 11 pDONR223-CSNK1A1L
  Spectinomycin
5 / E / 12 pDONR223-NAGK
  Spectinomycin
5 / F / 1 pDONR223-CDK7
  Spectinomycin
5 / F / 2 pDONR223-PRKACB
  Spectinomycin
5 / F / 3 pDONR223-PRKACG
  Spectinomycin
5 / F / 4 pDONR223-PRKAR2A
  Spectinomycin
5 / F / 5 pDONR223-MAPKAPK3
  Spectinomycin
5 / F / 6 pDONR223-CAMK1D
  Spectinomycin
5 / F / 7 pDONR223-ETNK2
  Spectinomycin
5 / F / 8 pDONR223-ADRBK2
  Spectinomycin
5 / F / 9 pDONR223-BCKDK
  Spectinomycin
5 / F / 10 pDONR223-STK32B
  Spectinomycin
5 / F / 11 pDONR223-CSNK1D
  Spectinomycin
5 / F / 12 pDONR223-CSNK1E
  Spectinomycin
5 / G / 1 pDONR223-MAP2K7
  Spectinomycin
5 / G / 2 pDONR223-IHPK2
  Spectinomycin
5 / G / 3 pDONR223-STK25
  Spectinomycin
5 / G / 4 pDONR223-PDPK1
  Spectinomycin
5 / G / 5 pDONR223-STK11
  Spectinomycin
5 / G / 6 pDONR223-STK40
  Spectinomycin
5 / G / 7 pDONR223-PDK1
  Spectinomycin
5 / G / 8 pDONR223-MAP2K5
  Spectinomycin
5 / G / 9 pDONR223-PIP5K3
  Spectinomycin
5 / G / 10 pDONR223-ILK
  Spectinomycin
5 / G / 11 pDONR223-PIK3R3
  Spectinomycin
5 / G / 12 pDONR223-ADPGK
  Spectinomycin
5 / H / 1 pDONR223-STK38L
  Spectinomycin
5 / H / 2 pDONR223-STK38
  Spectinomycin
5 / H / 3 pDONR223-CAMK4
  Spectinomycin
5 / H / 4 pDONR223-CAMK1G
  Spectinomycin
5 / H / 5 pDONR223-CAMK2D
  Spectinomycin
5 / H / 6 pDONR223-UCK2
  Spectinomycin
5 / H / 7 pDONR223-NEK11
  Spectinomycin
5 / H / 8 pDONR223-CLK1
  Spectinomycin
5 / H / 9 pDONR223-STK3
  Spectinomycin
5 / H / 10 pDONR223-PIK3R5
  Spectinomycin
5 / H / 11 pDONR223-DGUOK
  Spectinomycin
5 / H / 12 pDONR223-MAPK14
  Spectinomycin
6 / A / 1 pDONR223-ERBB3
  Spectinomycin
6 / A / 2 pDONR223-ERBB4
  Spectinomycin
6 / A / 3 pDONR223-FES
  Spectinomycin
6 / A / 4 pDONR223-FGR
  Spectinomycin
6 / A / 5 pDONR223-FRK
  Spectinomycin
6 / A / 6 pDONR223-HCK
  Spectinomycin
6 / A / 7 pDONR223-CKM
  Spectinomycin
6 / A / 8 pDONR223-ITK
  Spectinomycin
6 / A / 9 pDONR223-MATK
  Spectinomycin
6 / A / 10 pDONR223-NTRK2
  Spectinomycin
6 / A / 11 pDONR223-PKMYT1
  Spectinomycin
6 / A / 12 pDONR223-TEC
  Spectinomycin
6 / B / 1 pDONR223-TXK
  Spectinomycin
6 / B / 2 pDONR223-ZAP70
  Spectinomycin
6 / B / 3 pDONR223-ERBB2
  Spectinomycin
6 / B / 4 pDONR223-MET
  Spectinomycin
6 / B / 5 pDONR223-LCK
  Spectinomycin
6 / B / 6 pDONR223-NTRK1
  Spectinomycin
6 / B / 7 pDONR223-PDGFRA
  Spectinomycin
6 / B / 8 pDONR223-PDGFRB
  Spectinomycin
6 / B / 9 pDONR223-TNK1
  Spectinomycin
6 / B / 10 pDONR223-FLT3
  Spectinomycin
6 / B / 11 pDONR223-EPHB4
  Spectinomycin
6 / B / 12 pDONR223-DDR2
  Spectinomycin
6 / C / 1 pDONR223-PTK2B
  Spectinomycin
6 / C / 2 pDONR223-PTK6
  Spectinomycin
6 / C / 3 pDONR223-MERTK
  Spectinomycin
6 / C / 4 pDONR223-NTRK3
  Spectinomycin
6 / C / 5 pDONR223-PTK2
  Spectinomycin
6 / C / 6 pDONR223-STYK1
  Spectinomycin
6 / C / 7 pDONR223-FGFR2
  Spectinomycin
6 / C / 8 pDONR223-LYN
  Spectinomycin
6 / C / 9 pDONR223-RET
  Spectinomycin
6 / C / 10 pDONR223-SYK
  Spectinomycin
6 / C / 11 pDONR223-TYK2
  Spectinomycin
6 / C / 12 pDONR223-ABL2
  Spectinomycin
6 / D / 1 pDONR223-DDR1
  Spectinomycin
6 / D / 2 pDONR223-EPHA3
  Spectinomycin
6 / D / 3 pDONR223-FLT1
  Spectinomycin
6 / D / 4 pDONR223-PLK1
  Spectinomycin
6 / D / 5 pDONR223-LMTK2
  Spectinomycin
6 / D / 6 pDONR223-JAK2
  Spectinomycin
6 / D / 7 pDONR223-TYRO3
  Spectinomycin
6 / D / 8 pDONR223-ALK
  Spectinomycin
6 / D / 9 pDONR223-BTK
  Spectinomycin
6 / D / 10 pDONR223-EPHA4
  Spectinomycin
6 / D / 11 pDONR223-EPHA6
  Spectinomycin
6 / D / 12 pDONR223-FER
  Spectinomycin
6 / E / 1 pDONR223-FGFR1
  Spectinomycin
6 / E / 2 pDONR223-FLT4
  Spectinomycin
6 / E / 3 pDONR223-INSRR
  Spectinomycin
6 / E / 4 pDONR223-KDR
  Spectinomycin
6 / E / 5 pDONR223-EPHA2
  Spectinomycin
6 / E / 6 pDONR223-ROR2
  Spectinomycin
6 / E / 7 pDONR223-CSF1R
  Spectinomycin
6 / E / 8 pDONR223-EPHA1
  Spectinomycin
6 / E / 9 pDONR223-EPHB1
  Spectinomycin
6 / E / 10 pDONR223-EPHB6
  Spectinomycin
6 / E / 11 pDONR223-JAK1
  Spectinomycin
6 / E / 12 pDONR223-FGFR3
  Spectinomycin
6 / F / 1 pDONR223-SRC
  Spectinomycin
6 / F / 2 pDONR223-EGFR
  Spectinomycin
6 / F / 3 pDONR223-BMX
  Spectinomycin
6 / F / 5 pDONR223-YES1
  Spectinomycin
6 / F / 6 pDONR223-ABL1
  Spectinomycin
6 / F / 7 pDONR223-BLK
  Spectinomycin
6 / F / 8 pDONR223-CSK
  Spectinomycin
6 / F / 9 pDONR223-MST1R
  Spectinomycin
6 / F / 10 pDONR223-PTK7
  Spectinomycin
6 / F / 11 pDONR223-JAK3
  Spectinomycin
6 / F / 12 pDONR223-AXL
  Spectinomycin
6 / G / 1 pDONR223-TIE1
  Spectinomycin
6 / G / 2 pDONR223-PAPSS1
  Spectinomycin
6 / G / 3 pDONR223-PFKP
  Spectinomycin
6 / G / 4 pDONR223-DAK
  Spectinomycin
6 / G / 5 pDONR223-PRKAA1
  Spectinomycin
6 / G / 6 pDONR223-GK
  Spectinomycin
6 / G / 7 pDONR223-ACVRL1
  Spectinomycin
Data from: CACHED @ 2014-12-18 20:29:46.274324

Control and Expression Vectors

A set of 5 control ORFs (eGFP, BFP, HcRed, LacZ, Luciferase) are available to be used as negative controls for experiments with this set. These plasmids are not included in the kit and must be ordered separately.

A set of 4 custom lentiviral vectors is available (pLX301, pLX302, pLX303, pLX304) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

There are 3 activated oncogene ORF vectors that can be used with this set as well: K-RAS V12, H-RAS V12, MEKDD.

Related links: To learn more about the 15,000+ ORFs contained in the entry clone collection, as well as the entire lentiviral expression collection (15,000+ ORFs) described in the Nature Method paper, please visit the ORFeome Collaboration Website. Plasmids from the full ORF collections can be obtained from ODF/HCC, DNASU, and ThermoFisher.