Human Kinase Open Reading Frame Collection
Center for Cancer Systems Biology (DFCI)
Broad Institute of Harvard and MIT
Read the interview with DFCI Scientists, William Hahn and Jesse Boehm, who created the kinase libary. Click Here.
The Center for Cancer Systems Biology (Dana Farber Cancer Institute)-Broad Human Kinase ORF collection from Addgene consists of 559 distinct human kinases and kinase-related protein ORFs in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity.
These plasmids have been described in
A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, et al. Nature Methods. 2011 Jun 26. PubMed 21706014.
COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, et al. Nature. 2010 Nov 24. PubMed 21107320.
Kinase ORFs were assembled from a number of sources; 56% were isolated as single cloned isolates from the ORFeome 5.1 collection (http://horfdb.dfci.harvard.edu); 31% were cloned from normal human tissue RNA (Ambion) by reverse transcription and subsequent PCR amplification adding Gateway® sequences; 11% were cloned into Entry vectors from templates provided by the Harvard Institute of Proteomics (HIP); 2% additional kinases were cloned into Entry vectors from templates obtained from collaborating laboratories. All ORFs are open (stop codons removed) except for 5 (MST1R, PTK7, JAK3, AXL, TIE1) which are closed (have stop codons).
Browse Within the Collection
Download Information and Plate Map PDF (included with shipment).
A set of 5 control ORFs (eGFP, BFP, HcRed, LacZ, Luciferase) are available to be used as negative controls for experiments with this set. These plasmids are not included in the kit and must be ordered separately.
A set of 4 custom lentiviral vectors is available (pLX301, pLX302, pLX303, pLX304) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Related links: To learn more about the 15,000+ ORFs contained in the entry clone collection, as well as the entire lentiviral expression collection (15,000+ ORFs) described in the Nature Method paper, please visit the ORFeome Collaboration Website. Plasmids from the full ORF collections can be obtained from DF/HCC, DNASU, and ThermoFisher.
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