Addgene - PSICORhp-mp53 Plasmid Data

Plasmid 26273: PSICORhp-mp53

Gene/insert name:
p53 shRNA
Insert size:
55
Species:
M. musculus (mouse)
Vector backbone:
pSicoR
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7560
Cloning site 5':
HpaI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
TGCAGGGGAAAGAATAGTAGAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Comments:

The shRNA is directed against the mouse p53 sequence 5'-GTACTCTCCTCCCCTCAAT-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct cell reprogramming is a stochastic process amenable to acceleration. Hanna et al (Nature. 2009 Dec 3. 462(7273):595-601. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26273" in your Materials and Methods section.