Addgene - pSicoR-Ef1a-mCh Plasmid Data

Plasmid 31847: pSicoR-Ef1a-mCh

Gene/insert name:
None
Vector backbone:
pSicoR-mCH
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #21907
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size (bp):
7484
Modifications to Backbone:
The pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
Promoter:
U6 for shRNA; Ef1alpha for mCherry
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pSicoR-Ef1a-mCh_Seq (application/pdf)
Supplemental document:
pSicoR-Ef1a-mCh_txt (text/plain)
Supplemental document:
pSicoR-Ef1a-mCh.gb (application/octet-stream)
Principal Investigator:
Bruce Conklin
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

The pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α.

The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Salomonis et al (Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31847" in your Materials and Methods section.