|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31847||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid #21907
- Backbone size (bp) 7484
Modifications to backboneThe pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
- Promoter U6 for shRNA; Ef1alpha for mCherry
Growth in Bacteria
Copy numberHigh Copy
The pSicoR-Ef1α-mCh lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α.
The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSicoR-Ef1a-mCh was a gift from Bruce Conklin (Addgene plasmid # 31847)
For your References section:Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. 10.1073/pnas.0912260107 PubMed 20498046
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.