1
Plasmid 31845: pSicoR-Ef1a-mCh-Puro
  • None

  • pSicoR-mCH
    (Search Vector Database)

  • Addgene plasmid #21907

  • Mammalian Expression, Lentiviral, RNAi, Cre/Lox

  • 8141

  • The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.

  • U6 for shRNA; Ef1alpha for mCherry-puromycin

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • View sequences (2)
  • View map

  • pSicoR-Ef1a-mCh-Puro_Seq (application/pdf)

  • pSicoR-Ef1a-mCh-Puro_txt (text/plain)

  • pSicoR-Ef1a-mCh-Puro.gb (application/octet-stream)

  • Bruce Conklin

  • UBMTA
    Clontech Limited Use Label License

Comments: 

The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Salomonis et al (Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31845" in your Materials and Methods section.