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pCMV(CAT)T7-SB100 Citations (54)

Plasmid Article: Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates.
Mates L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche T, Ivics Z, Izsvak Z Nat Genet. 2009 Jun;41(6):753-61. Epub 2009 May 3.
PubMed Journal

Articles Citing pCMV(CAT)T7-SB100

Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons. Ivics Z, Mates L, Yau TY, Landa V, Zidek V, Bashir S, Hoffmann OI, Hiripi L, Garrels W, Kues WA, Bosze Z, Geurts A, Pravenec M, Rulicke T, Izsvak Z. Nat Protoc. 2014 Apr;9(4):773-93. doi: 10.1038/nprot.2014.008. Epub 2014 Mar 13. PubMed
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons. Ivics Z, Hiripi L, Hoffmann OI, Mates L, Yau TY, Bashir S, Zidek V, Landa V, Geurts A, Pravenec M, Rulicke T, Bosze Z, Izsvak Z. Nat Protoc. 2014 Apr;9(4):794-809. doi: 10.1038/nprot.2014.009. Epub 2014 Mar 13. PubMed
Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons. Ivics Z, Garrels W, Mates L, Yau TY, Bashir S, Zidek V, Landa V, Geurts A, Pravenec M, Rulicke T, Kues WA, Izsvak Z. Nat Protoc. 2014 Apr;9(4):810-27. doi: 10.1038/nprot.2014.010. Epub 2014 Mar 13. PubMed
Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression. Wang J, Singh M, Sun C, Besser D, Prigione A, Ivics Z, Hurst LD, Izsvak Z. Nat Protoc. 2016 Feb;11(2):327-46. doi: 10.1038/nprot.2016.016. Epub 2016 Jan 21. PubMed
Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-Based Stable Gene Delivery. Sebe A, Ivics Z. Methods Mol Biol. 2016;1400:419-27. doi: 10.1007/978-1-4939-3372-3_26. PubMed
Increased risk of interstitial lung disease in children with a single R288K variant of ABCA3. Wittmann T, Frixel S, Hoppner S, Schindlbeck U, Schams A, Kappler MV, Hegermann J, Wrede C, Liebisch G, Vierzig A, Zacharasiewicz A, Kopp M, Poets CF, Baden W, Hartl D, Van Kaam AH, Lohse P, Aslanidis C, Zarbock R, Griese M. Mol Med. 2016 Feb 26. pii: molmed.2015.00244. doi: 10.2119/molmed.2015.00244. PubMed
Sleeping Beauty transposon-based system for rapid generation of HBV-replicating stable cell lines. Wu Y, Zhang TY, Fang LL, Chen ZX, Song LW, Cao JL, Yang L, Yuan Q, Xia NS. J Virol Methods. 2016 Aug;234:96-100. doi: 10.1016/j.jviromet.2016.04.010. Epub 2016 Apr 22. PubMed
A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Kaberniuk AA, Shemetov AA, Verkhusha VV. Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864. PubMed

Associated Plasmids

Tools to explore ABCA3 mutations causing interstitial lung disease. Wittmann T, Schindlbeck U, Hoppner S, Kinting S, Frixel S, Kroner C, Liebisch G, Hegermann J, Aslanidis C, Brasch F, Reu S, Lasch P, Zarbock R, Griese M. Pediatr Pulmonol. 2016 May 13. doi: 10.1002/ppul.23471. PubMed
Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing. Yum SY, Lee SJ, Kim HM, Choi WJ, Park JH, Lee WW, Kim HS, Kim HJ, Bae SH, Lee JH, Moon JY, Lee JH, Lee CI, Son BJ, Song SH, Ji SM, Kim SJ, Jang G. Sci Rep. 2016 Jun 21;6:27185. doi: 10.1038/srep27185. PubMed
Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors. Monjezi R, Miskey C, Gogishvili T, Schleef M, Schmeer M, Einsele H, Ivics Z, Hudecek M. Leukemia. 2017 Jan;31(1):186-194. doi: 10.1038/leu.2016.180. Epub 2016 Jun 24. PubMed
Four Novel Splice-Switch Reporter Cell Lines: Distinct Impact of Oligonucleotide Chemistry and Delivery Vector on Biological Activity. Rocha CS, Lundin KE, Behlke MA, Zain R, El Andaloussi S, Smith CI. Nucleic Acid Ther. 2016 Dec;26(6):381-391. Epub 2016 Sep 15. PubMed
A vector platform for the rapid and efficient engineering of stable complex transgenes. Jackel C, Nogueira MS, Ehni N, Kraus C, Ranke J, Dohmann M, Noessner E, Nelson PJ. Sci Rep. 2016 Oct 3;6:34365. doi: 10.1038/srep34365. PubMed

Associated Plasmids

Transposon-mediated Generation of Cellular and Mouse Models of Splicing Mutations to Assess the Efficacy of snRNA-based Therapeutics. Barbon E, Ferrarese M, van Wittenberghe L, Sanatine P, Ronzitti G, Collaud F, Colella P, Pinotti M, Mingozzi F. Mol Ther Nucleic Acids. 2016 Nov 29;5(11):e392. doi: 10.1038/mtna.2016.97. PubMed
A Novel Ideal Radionuclide Imaging System for Non-invasively Cell Monitoring built on Baculovirus Backbone by Introducing Sleeping Beauty Transposon. Lv J, Pan Y, Ju H, Zhou J, Cheng D, Shi H, Zhang Y. Sci Rep. 2017 Mar 6;7:43879. doi: 10.1038/srep43879. PubMed
Self-organized developmental patterning and differentiation in cerebral organoids. Renner M, Lancaster MA, Bian S, Choi H, Ku T, Peer A, Chung K, Knoblich JA. EMBO J. 2017 May 15;36(10):1316-1329. doi: 10.15252/embj.201694700. Epub 2017 Mar 10. PubMed
CRISPR/Cas9-based Pten knock-out and Sleeping Beauty Transposon-mediated Nras knock-in induces hepatocellular carcinoma and hepatic lipid accumulation in mice. Gao M, Liu D. Cancer Biol Ther. 2017 May 17:1-8. doi: 10.1080/15384047.2017.1323597. PubMed
Guided self-organization and cortical plate formation in human brain organoids. Lancaster MA, Corsini NS, Wolfinger S, Gustafson EH, Phillips AW, Burkard TR, Otani T, Livesey FJ, Knoblich JA. Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31. PubMed

Associated Plasmids

Integration of Oncogenes via Sleeping Beauty as a Mouse Model of HPV16(+) Oral Tumors and Immunologic Control. Lin YH, Yang MC, Tseng SH, Jiang R, Yang A, Farmer E, Peng S, Henkle T, Chang YN, Hung CF, Wu TC. Cancer Immunol Res. 2018 Mar;6(3):305-319. doi: 10.1158/2326-6066.CIR-16-0358. Epub 2018 Jan 23. PubMed
Establishment of Cre-mediated HBV recombinant cccDNA (rcccDNA) cell line for cccDNA biology and antiviral screening assays. Wu M, Li J, Yue L, Bai L, Li Y, Chen J, Zhang X, Yuan Z. Antiviral Res. 2018 Apr;152:45-52. doi: 10.1016/j.antiviral.2018.02.007. Epub 2018 Feb 9. PubMed
Estrogen-dependent control and cell-to-cell variability of transcriptional bursting. Fritzsch C, Baumgartner S, Kuban M, Steinshorn D, Reid G, Legewie S. Mol Syst Biol. 2018 Feb 23;14(2):e7678. doi: 10.15252/msb.20177678. PubMed
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA. Cell Stem Cell. 2018 Apr 5;22(4):543-558.e12. doi: 10.1016/j.stem.2018.03.002. PubMed
Novel lymphoid enhancer-binding factor 1-cytoglobin axis promotes extravasation of osteosarcoma cells into the lungs. Pongsuchart M, Kuchimaru T, Yonezawa S, Tran DTP, Kha NT, Hoang NTH, Kadonosono T, Kizaka-Kondoh S. Cancer Sci. 2018 Sep;109(9):2746-2756. doi: 10.1111/cas.13702. Epub 2018 Jul 24. PubMed
Genetically engineered cerebral organoids model brain tumor formation. Bian S, Repic M, Guo Z, Kavirayani A, Burkard T, Bagley JA, Krauditsch C, Knoblich JA. Nat Methods. 2018 Aug;15(8):631-639. doi: 10.1038/s41592-018-0070-7. Epub 2018 Jul 23. PubMed
A reliable murine model of bone metastasis by injecting cancer cells through caudal arteries. Kuchimaru T, Kataoka N, Nakagawa K, Isozaki T, Miyabara H, Minegishi M, Kadonosono T, Kizaka-Kondoh S. Nat Commun. 2018 Jul 30;9(1):2981. doi: 10.1038/s41467-018-05366-3. PubMed
Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Li Y, Hermanson DL, Moriarity BS, Kaufman DS. Cell Stem Cell. 2018 Aug 2;23(2):181-192.e5. doi: 10.1016/j.stem.2018.06.002. Epub 2018 Jun 28. PubMed
A multi-state model of chemoresistance to characterize phenotypic dynamics in breast cancer. Howard GR, Johnson KE, Rodriguez Ayala A, Yankeelov TE, Brock A. Sci Rep. 2018 Aug 13;8(1):12058. doi: 10.1038/s41598-018-30467-w. PubMed
Expression and characterization of soluble epitope-defined major histocompatibility complex (MHC) from stable eukaryotic cell lines. Wooster AL, Anderson TS, Lowe DB. J Immunol Methods. 2019 Jan;464:22-30. doi: 10.1016/j.jim.2018.10.006. Epub 2018 Oct 19. PubMed
Purity by design: Reducing impurities in bioproduction by stimulus-controlled global translational downregulation of non-product proteins. Bojar D, Fuhrer T, Fussenegger M. Metab Eng. 2019 Mar;52:110-123. doi: 10.1016/j.ymben.2018.11.007. Epub 2018 Nov 20. PubMed
A novel spontaneous hepatocellular carcinoma mouse model for studying T-cell exhaustion in the tumor microenvironment. Liu YT, Tseng TC, Soong RS, Peng CY, Cheng YH, Huang SF, Chuang TH, Kao JH, Huang LR. J Immunother Cancer. 2018 Dec 7;6(1):144. doi: 10.1186/s40425-018-0462-3. PubMed
High-performance gene expression and knockout tools using sleeping beauty transposon system. Hu K, Li Y, Wu W, Chen H, Chen Z, Zhang Y, Guo Y, Dong Y. Mob DNA. 2018 Nov 26;9:33. doi: 10.1186/s13100-018-0139-y. eCollection 2018. PubMed
Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C. Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0. PubMed

Associated Plasmids

Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB. Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PubMed

Associated Plasmids

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network. Shivram H, Le SV, Iyer VR. Genome Res. 2019 Feb;29(2):184-192. doi: 10.1101/gr.238311.118. Epub 2019 Jan 16. PubMed
Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Zhang S, Chen T, Chen N, Gao D, Shi B, Kong S, West RC, Yuan Y, Zhi M, Wei Q, Xiang J, Mu H, Yue L, Lei X, Wang X, Zhong L, Liang H, Cao S, Belmonte JCI, Wang H, Han J. Nat Commun. 2019 Jan 30;10(1):496. doi: 10.1038/s41467-019-08378-9. PubMed
Ribosomal Proteins Regulate MHC Class I Peptide Generation for Immunosurveillance. Wei J, Kishton RJ, Angel M, Conn CS, Dalla-Venezia N, Marcel V, Vincent A, Catez F, Ferre S, Ayadi L, Marchand V, Dersh D, Gibbs JS, Ivanov IP, Fridlyand N, Coute Y, Diaz JJ, Qian SB, Staudt LM, Restifo NP, Yewdell JW. Mol Cell. 2019 Mar 21;73(6):1162-1173.e5. doi: 10.1016/j.molcel.2018.12.020. Epub 2019 Jan 31. PubMed
Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output. Giandomenico SL, Mierau SB, Gibbons GM, Wenger LMD, Masullo L, Sit T, Sutcliffe M, Boulanger J, Tripodi M, Derivery E, Paulsen O, Lakatos A, Lancaster MA. Nat Neurosci. 2019 Apr;22(4):669-679. doi: 10.1038/s41593-019-0350-2. Epub 2019 Mar 18. PubMed
A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics. Dersh D, Yewdell JW, Wei J. Methods Mol Biol. 2019;1988:109-122. doi: 10.1007/978-1-4939-9450-2_9. PubMed
Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet beta-cells. Bearrows SC, Bauchle CJ, Becker M, Haldeman JM, Swaminathan S, Stephens SB. J Cell Sci. 2019 Jul 1;132(13). pii: jcs.231373. doi: 10.1242/jcs.231373. PubMed
Potential limitations of the Sleeping Beauty transposon use in gene expression studies. Sowinska W, Wawro M, Solecka A, Kasza A. Acta Biochim Pol. 2019 Jul 12;66(3):263-268. doi: 10.18388/abp.2019_2839. PubMed
Cancer cell population growth kinetics at low densities deviate from the exponential growth model and suggest an Allee effect. Johnson KE, Howard G, Mo W, Strasser MK, Lima EABF, Huang S, Brock A. PLoS Biol. 2019 Aug 5;17(8):e3000399. doi: 10.1371/journal.pbio.3000399. eCollection 2019 Aug. PubMed
Elevated UCP1 levels are sufficient to improve glucose uptake in human white adipocytes. Tews D, Pula T, Funcke JB, Jastroch M, Keuper M, Debatin KM, Wabitsch M, Fischer-Posovszky P. Redox Biol. 2019 Sep;26:101286. doi: 10.1016/j.redox.2019.101286. Epub 2019 Jul 27. PubMed
Systematic quantification of the anion transport function of pendrin (SLC26A4) and its disease-associated variants. Wasano K, Takahashi S, Rosenberg SK, Kojima T, Mutai H, Matsunaga T, Ogawa K, Homma K. Hum Mutat. 2020 Jan;41(1):316-331. doi: 10.1002/humu.23930. Epub 2019 Oct 26. PubMed
Induction of metabolic quiescence defines the transitional to follicular B cell switch. Farmer JR, Allard-Chamard H, Sun N, Ahmad M, Bertocchi A, Mahajan VS, Aicher T, Arnold J, Benson MD, Morningstar J, Barmettler S, Yuen G, Murphy SJH, Walter JE, Ghebremichael M, Shalek AK, Batista F, Gerszten R, Pillai S. Sci Signal. 2019 Oct 22;12(604). pii: 12/604/eaaw5573. doi: 10.1126/scisignal.aaw5573. PubMed
Malignant ascites: a source of therapeutic protein against ovarian cancer? Ribaux P, Britan A, Thumann G, Delie F, Petignat P, Cohen M. Oncotarget. 2019 Oct 15;10(57):5894-5905. doi: 10.18632/oncotarget.27185. eCollection 2019 Oct 15. PubMed
Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer. Krasnov GS, Puzanov GA, Afanasyeva MA, Dashinimaev EB, Vishnyakova KS, Beniaminov AD, Adzhubei AA, Kondratieva TT, Yegorov YE, Senchenko VN. Biosci Rep. 2019 Dec 20;39(12). pii: 221348. doi: 10.1042/BSR20193094. PubMed
Individual Cells Can Resolve Variations in Stimulus Intensity along the IGF-PI3K-AKT Signaling Axis. Gross SM, Dane MA, Bucher E, Heiser LM. Cell Syst. 2019 Dec 18;9(6):580-588.e4. doi: 10.1016/j.cels.2019.11.005. Epub 2019 Dec 11. PubMed
Proteolytic Processing of Neuregulin 2. Czarnek M, Bereta J. Mol Neurobiol. 2019 Dec 14. pii: 10.1007/s12035-019-01846-9. doi: 10.1007/s12035-019-01846-9. PubMed
Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. PubMed

Associated Plasmids

CD226(hi)CD8(+) T Cells Are a Prerequisite for Anti-TIGIT Immunotherapy. Jin HS, Ko M, Choi DS, Kim JH, Lee DH, Kang SH, Kim I, Lee HJ, Choi EK, Kim KP, Yoo C, Park Y. Cancer Immunol Res. 2020 Jul;8(7):912-925. doi: 10.1158/2326-6066.CIR-19-0877. Epub 2020 Apr 7. PubMed
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. Hegarat N, Crncec A, Suarez Peredo Rodriguez MF, Echegaray Iturra F, Gu Y, Busby O, Lang PF, Barr AR, Bakal C, Kanemaki MT, Lamond AI, Novak B, Ly T, Hochegger H. EMBO J. 2020 Jun 2;39(11):e104419. doi: 10.15252/embj.2020104419. Epub 2020 Apr 30. PubMed
Tracking of an Oral Salmonella-Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice. Mbongue JC, Alhoshani A, Rawson J, Garcia PA, Gonzalez N, Ferreri K, Kandeel F, Husseiny MI. Front Immunol. 2020 Apr 28;11:712. doi: 10.3389/fimmu.2020.00712. eCollection 2020. PubMed
Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation. Stiff T, Echegaray-Iturra FR, Pink HJ, Herbert A, Reyes-Aldasoro CC, Hochegger H. Cell Rep. 2020 May 26;31(8):107681. doi: 10.1016/j.celrep.2020.107681. PubMed
METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue. Laptev I, Shvetsova E, Levitskii S, Serebryakova M, Rubtsova M, Zgoda V, Bogdanov A, Kamenski P, Sergiev P, Dontsova O. Nucleic Acids Res. 2020 Aug 20;48(14):8022-8034. doi: 10.1093/nar/gkaa522. PubMed

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