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pRetroQ-Cre-ERT2
(Plasmid #59701)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59701 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRetroQ-AcGFP1-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6886
  • Total vector size (bp) 8870
  • Modifications to backbone
    AcGFP1 is removed during cloning. Cre-ERT2 is cloned into NheI/EcoRI site by Gibson assembly
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cre-ERT2
  • Alt name
    inducible Cre
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    1983
  • Promoter cmvIE
  • Tag / Fusion Protein
    • Estrogen receptor (ERT) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGT CTA TAT AAG CAG AGC TG
  • 3′ sequencing primer CGAAGGGGCCACCAAAGAAC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

ERT2-Cre-ERT2 in the same retrovirus vector (pRetroQ) has too low activity in stable cell line to prevent it to be useful. pRetroQ-Cre-ERT2 made stable cell line exhibits no leaky Cre activity whereas pCAG-Cre-ERT2 transient expression does lead to leaky Cre activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroQ-Cre-ERT2 was a gift from Richard Youle (Addgene plasmid # 59701 ; http://n2t.net/addgene:59701 ; RRID:Addgene_59701)