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Broad Target Accelerator Plasmid Collections
(Kit # 1000000103, 1000000104, 1000000105 )

Depositing Labs: Jesse Boehm, William Hahn, Matthew Meyerson, and David Root

Clinical sequencing of human cancers has revealed large numbers of rare, poorly characterized mutations. However, the traditional approach of elucidating disease allele function involves detailed one-by-one investigation. While these efforts have been incredibly powerful, the throughput of such investigation does not match the pace of genetic discovery today.

The Broad Institute’s Target Accelerator effort aims to tackle this challenge and make it possible to functionally characterize the cancer genome in a high throughput fashion. A series of pilot projects aims to evaluate whether integrating experimental and computational approaches indeed accelerates the process by which mutant alleles become nominated and provisionally credentialed as targets.

The wild-type and mutant alleles featured in this collection focus on somatic variants found in lung adenocarcinoma as well as variants found across cancer types as part of “pan-cancer” analyses. These plasmids can be purchased individually or as part of a series of kits, collectively called the Broad Target Accelerator Plasmid Collections.

These kits will be sent as bacterial glycerol stocks in a 96-well plate format.

These pilot studies were generously funded through the Cancer Target Discovery and Development (CTD2) network, U.S. NCI grant U01 CA176058, and via the Slim Initiative for Genomic Medicine, a project funded by the Carlos Slim Foundation in Mexico.

Ordering

Item Catalog # Description Quantity Price (USD)
Kit 1000000103 Target Accelerator Pan-Cancer Mutant Collection 1 $1750 Add to Cart
Kit 1000000104 Target Accelerator Lung Cancer Mutant Collection 1 $1050 Add to Cart
Kit 1000000105 Target Accelerator Genetic Pathway Reference Set 1 $800 Add to Cart
This material is available to academics and nonprofits only.

Description

In the Target Accelerator Plasmid Collections, you can find the wild-type and mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across cancer types as part of “pan-cancer” analyses.

Publication List for Kits:

Kit #1000000103: Target Accelerator Pan-Cancer Mutant Collection

The Target Accelerator Pan-Cancer Mutant Collection consists of a total of 631 plasmids used in the experiments reported in Kim et al., 2016. Overall, these include 454 mutant clones and 177 corresponding wild-type clones. For a description of the methods used to nominate these mutations and genes, please refer to the methods section of Kim et al., 2016.

Each clone was derived from the hORFeome 8.1 collection of wild-type human ORFs ( Yang X, et al., 2011). cDNA clones with perfect or near-perfect matches to REFseq templates were used in Suzuki-based stitching PCR reactions to create mutant variants in the Gateway compatible pDONR223 vector as depicted in the graphic below.

Suzuki-based stitching PCR process

Variants were sequence confirmed using Sanger sequencing, and LR reactions transferred these into pLX-based destination vectors for the experiments described in the papers (see Control Vectors Tab above). Note: the Kim et al. paper also describes the creation of the Target Accelerator Genetic Pathway Reference Set (see Kit #1000000105 below).

Kit #1000000104: Target Accelerator Lung Cancer Mutant Collection

The Lung Cancer Mutation Collection consists of a total of 307 plasmids used in the experiments reported in Berger et al., 2016. Overall, these include 180 mutant plasmids, 45 wild-type plasmids corresponding to these lung cancer genes and 82 wild-type plasmids serving as expression controls for the L1000 experiments reported in the paper. For more information regarding the rationale and selection process for the clones used, please see the methods section of Berger et al., 2016.

Mutant plasmids were created and verified as described above for the pan-cancer clones and transferred into the pLX317 destination vector. This also applies to the 5 control plasmids encoding BFP, eGFP, HcRed, Luciferase, and LacZ ORFs listed above.

Kit #1000000105: Target Accelerator Genetic Pathway Reference Set

The Genetic Pathway Reference Collection consists of 182 wild-type and 19 well-characterized mutant ORFs that represent over 25 cellular signaling pathways and processes. These clones are a high-quality, well annotated collection of genes and mutations that can be used as reference pathway signatures and phenotypes to compare with those derived from cancer alleles.

Control and Lentiviral Expression Vectors

These plasmids are not included in the kits and have to be ordered separately.

A set of 5 control ORFs are available to be used as negative controls for experiments with this collection:

A set of lentiviral vectors is available to express these ORFs in mammalian cells:

To learn more about the 15,000+ ORFs contained in the entry clone collection that was used to generate the mutant clones, as well as the entire lentiviral expression collection (15,000+ ORFs) described in the Yang X, et al. Nature Methods paper, please visit the ORFeome Collaboration Website. Plasmids from the full ORF collections can be obtained from ODF/HCC, DNASU, and Dharmacon.

Broad Target Accelerator Plasmid Collection References

Target Accelerator References

Systematic functional interrogation of rare cancer variants identifies oncogenic alleles. Kim E, et al. Cancer Discovery, 2016, Jul;6(7):714-26. PubMed PMID 27147599

High-throughput phenotyping of lung cancer somatic mutations. Berger AH, et al. Cancer Cell, 2016, Aug 8;30(2):214-28. PubMed PMID 27478040

Supporting References

Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban MH, et al. Elife, 2017, Mar 18;6. pii: e24060. PubMed PMID 28315521

Progress towards precision functional genomics in cancer. Johannessen CM and Boehm JS. Current Opinion in Systems Biology, 2017, 2:73–82. Link

A public genome-scale lentiviral expression library of human ORFs. Yang X, et al. Nature Methods, 2011, Jun 26;8(8):659-61. PubMed PMID 21706014

How to Cite these Kits

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Target Accelerator Plasmid Collection was a gift from Jesse Boehm (Addgene kit #100000010X).”

Target Accelerator Pan-Cancer Mutant Collection - #1000000103

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pDONR223_SPATA8_p.P83T
Spectinomycin
Plate 1 / A / 2 pDONR223_RHOA_p.Y42S
Spectinomycin
Plate 1 / A / 3 pDONR223_SPANXN4_p.P4Q
Spectinomycin
Plate 1 / A / 4 pDONR223_B2M_p.A6V
Spectinomycin
Plate 1 / A / 5 pDONR223_PDZD11_p.R133H
Spectinomycin
Plate 1 / A / 6 pDONR223_REG1A_p.S131I
Spectinomycin
Plate 1 / A / 7 pDONR223_HRAS_p.G12C
Spectinomycin
Plate 1 / A / 8 pDONR223_CBFB_p.R151H
Spectinomycin
Plate 1 / A / 9 pDONR223_RHOA_p.G62E
Spectinomycin
Plate 1 / A / 10 pDONR223_HIST1H1E_p.A60D
Spectinomycin
Plate 1 / A / 11 pDONR223_KLRC2_p.G174R
Spectinomycin
Plate 1 / A / 12 pDONR223_WISP2_p.R230L
Spectinomycin
Plate 1 / B / 1 pDONR223_SPATA8_p.E18K
Spectinomycin
Plate 1 / B / 2 pDONR223_RAB42_p.A61T
Spectinomycin
Plate 1 / B / 3 pDONR223_B2M_p.M1L
Spectinomycin
Plate 1 / B / 4 pDONR223_H2AFX_p.K134N
Spectinomycin
Plate 1 / B / 5 pDONR223_REG1B_p.A82T
Spectinomycin
Plate 1 / B / 6 pDONR223_HRAS_p.G12A
Spectinomycin
Plate 1 / B / 7 pDONR223_FTH1_p.A161T
Spectinomycin
Plate 1 / B / 8 pDONR223_RHOA_p.S85F
Spectinomycin
Plate 1 / B / 9 pDONR223_B2M_p.M1R
Spectinomycin
Plate 1 / B / 10 pDONR223_B2M_p.M1V
Spectinomycin
Plate 1 / B / 11 pDONR223_EIF1AX_p.G9R
Spectinomycin
Plate 1 / B / 12 pDONR223_REG1B_p.K157T
Spectinomycin
Plate 1 / C / 1 pDONR223_HRAS_p.G12D
Spectinomycin
Plate 1 / C / 2 pDONR223_TRAT1_p.E76K
Spectinomycin
Plate 1 / C / 3 pDONR223_RHOA_p.F106L
Spectinomycin
Plate 1 / C / 4 pDONR223_OTUD6A_p.R171P
Spectinomycin
Plate 1 / C / 5 pDONR223_C19orf48_p.L70F
Spectinomycin
Plate 1 / C / 6 pDONR223_B2M_p.M1I
Spectinomycin
Plate 1 / C / 7 pDONR223_REG1B_p.P30S
Spectinomycin
Plate 1 / C / 8 pDONR223_HRAS_p.A59T
Spectinomycin
Plate 1 / C / 9 pDONR223_TRAT1_p.D40Y
Spectinomycin
Plate 1 / C / 10 pDONR223_RHOA_p.R5Q
Spectinomycin
Plate 1 / C / 11 pDONR223_REG1A_p.P105T
Spectinomycin
Plate 1 / C / 12 pDONR223_LCE1B_p.S42F
Spectinomycin
Plate 1 / D / 1 pDONR223_IER3_p.A155T
Spectinomycin
Plate 1 / D / 2 pDONR223_REG1B_p.C64W
Spectinomycin
Plate 1 / D / 3 pDONR223_REG3A_p.G85R
Spectinomycin
Plate 1 / D / 4 pDONR223_RAC1_p.P29S
Spectinomycin
Plate 1 / D / 5 pDONR223_DNAJC12_p.R178C
Spectinomycin
Plate 1 / D / 6 pDONR223_HRAS_p.Q61L
Spectinomycin
Plate 1 / D / 7 pDONR223_B2M_p.D54N
Spectinomycin
Plate 1 / D / 8 pDONR223_C1orf150_p.Y89N
Spectinomycin
Plate 1 / D / 9 pDONR223_HRAS_p.A11D
Spectinomycin
Plate 1 / D / 10 pDONR223_RHOA_p.Y42C
Spectinomycin
Plate 1 / D / 11 pDONR223_CDKN1B_p.C29F
Spectinomycin
Plate 1 / D / 12 pDONR223_B2M_p.C100S
Spectinomycin
Plate 1 / E / 1 pDONR223_C1orf150_p.G2E
Spectinomycin
Plate 1 / E / 2 pDONR223_PPIAL4G_p.N35H
Spectinomycin
Plate 1 / E / 3 pDONR223_HRAS_p.Q61R
Spectinomycin
Plate 1 / E / 4 pDONR223_BTC_p.P70S
Spectinomycin
Plate 1 / E / 5 pDONR223_RHOA_p.E40Q
Spectinomycin
Plate 1 / E / 6 pDONR223_CD3E_p.E189Q
Spectinomycin
Plate 1 / E / 7 pDONR223_REG3A_p.P109S
Spectinomycin
Plate 1 / E / 8 pDONR223_B2M_p.Y30H
Spectinomycin
Plate 1 / E / 9 pDONR223_HIST1H3B_p.A48S
Spectinomycin
Plate 1 / E / 10 pDONR223_REG1A_p.E80K
Spectinomycin
Plate 1 / E / 11 pDONR223_HRAS_p.Q61K
Spectinomycin
Plate 1 / E / 12 pDONR223_GCET2_p.H177Q
Spectinomycin
Plate 1 / F / 1 pDONR223_RHOA_p.T175M
Spectinomycin
Plate 1 / F / 2 pDONR223_CD79B_p.H194N
Spectinomycin
Plate 1 / F / 3 pDONR223_ISX_p.T87A
Spectinomycin
Plate 1 / F / 4 pDONR223_OR2T33_p.W49L
Spectinomycin
Plate 1 / F / 5 pDONR223_GZMA_p.G210E
Spectinomycin
Plate 1 / F / 6 pDONR223_CCNG1_p.E167K
Spectinomycin
Plate 1 / F / 7 pDONR223_CDK4_p.A23S
Spectinomycin
Plate 1 / F / 8 pDONR223_OR2G6_p.N42S
Spectinomycin
Plate 1 / F / 9 pDONR223_OR2T33_p.P285L
Spectinomycin
Plate 1 / F / 10 pDONR223_ELF3_p.A332V
Spectinomycin
Plate 1 / F / 11 pDONR223_OR2T33_p.L60P
Spectinomycin
Plate 1 / F / 12 pDONR223_GZMA_p.H38N
Spectinomycin
Plate 1 / G / 1 pDONR223_CPXCR1_p.A56S
Spectinomycin
Plate 1 / G / 2 pDONR223_PPP6C_p.E180K
Spectinomycin
Plate 1 / G / 3 pDONR223_OR2G6_p.I177F
Spectinomycin
Plate 1 / G / 4 pDONR223_OR2T33_p.W147C
Spectinomycin
Plate 1 / G / 5 pDONR223_MAGEC2_p.S85I
Spectinomycin
Plate 1 / G / 6 pDONR223_PSG2_p.S265C
Spectinomycin
Plate 1 / G / 7 pDONR223_PIK3IP1_p.G185E
Spectinomycin
Plate 1 / G / 8 pDONR223_CPXCR1_p.S102P
Spectinomycin
Plate 1 / G / 9 pDONR223_PPP6C_p.R264C
Spectinomycin
Plate 1 / G / 10 pDONR223_OR2G6_p.K236M
Spectinomycin
Plate 1 / G / 11 pDONR223_OR2T33_p.R120S
Spectinomycin
Plate 1 / G / 12 pDONR223_TNF_p.S157R
Spectinomycin
Plate 1 / H / 1 pDONR223_PRR23B_p.E140K
Spectinomycin
Plate 1 / H / 2 pDONR223_CPXCR1_p.E73K
Spectinomycin
Plate 1 / H / 3 pDONR223_GPR141_p.E267K
Spectinomycin
Plate 1 / H / 4 pDONR223_OR2G6_p.N294Y
Spectinomycin
Plate 1 / H / 5 pDONR223_GPS2_p.R102Q
Spectinomycin
Plate 1 / H / 6 pDONR223_GNA13_p.L374I
Spectinomycin
Plate 1 / H / 7 pDONR223_PRR23B_p.H245Q
Spectinomycin
Plate 1 / H / 8 pDONR223_RBM11_p.R74C
Spectinomycin
Plate 1 / H / 9 pDONR223_OR2G6_p.W149C
Spectinomycin
Plate 1 / H / 10 pDONR223_PSG2_p.H245Y
Spectinomycin
Plate 1 / H / 11 pDONR223_SERPINB4_p.A137E
Spectinomycin
Plate 1 / H / 12 pDONR223_TP53_p.C176F
Spectinomycin
Plate 2 / A / 1 pDONR223_GPR141_p.Q224K
Spectinomycin
Plate 2 / A / 2 pDONR223_U2AF1_p.S34F
Spectinomycin
Plate 2 / A / 3 pDONR223_PRR23B_p.A246T
Spectinomycin
Plate 2 / A / 4 pDONR223_TNFRSF14_p.A102P
Spectinomycin
Plate 2 / A / 5 pDONR223_OR2G6_p.R265K
Spectinomycin
Plate 2 / A / 6 pDONR223_CYLC2_p.R71C
Spectinomycin
Plate 2 / A / 7 pDONR223_HRH4_p.S170L
Spectinomycin
Plate 2 / A / 8 pDONR223_GPR141_p.T6N
Spectinomycin
Plate 2 / A / 9 pDONR223_ISX_p.R86C
Spectinomycin
Plate 2 / A / 10 pDONR223_OTUD6A_p.V211M
Spectinomycin
Plate 2 / A / 11 pDONR223_NFKBIA_p.P137S
Spectinomycin
Plate 2 / A / 12 pDONR223_C15orf23_p.P26S
Spectinomycin
Plate 2 / B / 1 pDONR223_MFAP4_p.Y55S
Spectinomycin
Plate 2 / B / 2 pDONR223_HAUS1_p.A102V
Spectinomycin
Plate 2 / B / 3 pDONR223_FBXO16_p.T50K
Spectinomycin
Plate 2 / B / 4 pDONR223_C15orf23_p.P26L
Spectinomycin
Plate 2 / B / 5 pDONR223_OR2T33_p.F164V
Spectinomycin
Plate 2 / B / 6 pDONR223_DNASE2_p.Y182H
Spectinomycin
Plate 2 / B / 7 pDONR223_ILKAP_p.S61Y
Spectinomycin
Plate 2 / B / 8 pDONR223_WNT2_p.V334M
Spectinomycin
Plate 2 / B / 9 pDONR223_ZDHHC4_p.R174H
Spectinomycin
Plate 2 / B / 10 pDONR223_TP53_p.S215I
Spectinomycin
Plate 2 / B / 11 pDONR223_HERPUD2_p.R285Q
Spectinomycin
Plate 2 / B / 12 pDONR223_TP53_p.C275S
Spectinomycin
Plate 2 / C / 1 pDONR223_C20orf72_p.P102S
Spectinomycin
Plate 2 / C / 2 pDONR223_SERPINB4_p.F182I
Spectinomycin
Plate 2 / C / 3 pDONR223_IKZF5_p.E135K
Spectinomycin
Plate 2 / C / 4 pDONR223_CYLC2_p.E133K
Spectinomycin
Plate 2 / C / 5 pDONR223_MAGEC2_p.R287W
Spectinomycin
Plate 2 / C / 6 pDONR223_SERPINB4_p.E290K
Spectinomycin
Plate 2 / C / 7 pDONR223_CYLC2_p.E152K
Spectinomycin
Plate 2 / C / 8 pDONR223_MAGEC2_p.E269K
Spectinomycin
Plate 2 / C / 9 pDONR223_SERPINB4_p.E110K
Spectinomycin
Plate 2 / C / 10 pDONR223_SGK1_p.F27L
Spectinomycin
Plate 2 / C / 11 pDONR223_CYLC2_p.V105F
Spectinomycin
Plate 2 / C / 12 pDONR223_MAGEC2_p.G74V
Spectinomycin
Plate 2 / D / 1 pDONR223_SERPINB4_p.T347N
Spectinomycin
Plate 2 / D / 2 pDONR223_TP53_p.I195F
Spectinomycin
Plate 2 / D / 3 pDONR223_A4GALT_p.A272V
Spectinomycin
Plate 2 / D / 4 pDONR223_ACTG1_p.K61E
Spectinomycin
Plate 2 / D / 5 pDONR223_HRH4_p.S115L
Spectinomycin
Plate 2 / D / 6 pDONR223_TP53_p.C275F
Spectinomycin
Plate 2 / D / 7 pDONR223_TP53_p.M246R
Spectinomycin
Plate 2 / D / 8 pDONR223_STK11_p.D194Y
Spectinomycin
Plate 2 / D / 9 pDONR223_EDNRB_p.F292L
Spectinomycin
Plate 2 / D / 10 pDONR223_IDH2_p.E268D
Spectinomycin
Plate 2 / D / 11 pDONR223_DCAF4L2_p.P160L
Spectinomycin
Plate 2 / D / 12 pDONR223_FBXO15_p.E410K
Spectinomycin
Plate 2 / E / 1 pDONR223_EDNRB_p.L349I
Spectinomycin
Plate 2 / E / 2 pDONR223_IDH2_p.G137E
Spectinomycin
Plate 2 / E / 3 pDONR223_DCAF4L2_p.A79V
Spectinomycin
Plate 2 / E / 4 pDONR223_FBXO15_p.S366G
Spectinomycin
Plate 2 / E / 5 pDONR223_GATA3_p.R330K
Spectinomycin
Plate 2 / E / 6 pDONR223_FAM196A_p.R399Q
Spectinomycin
Plate 2 / E / 7 pDONR223_DCAF4L2_p.L37H
Spectinomycin
Plate 2 / E / 8 pDONR223_HERPUD2_p.P46L
Spectinomycin
Plate 2 / E / 9 pDONR223_CNBD1_p.L396R
Spectinomycin
Plate 2 / E / 10 pDONR223_WIPI1_p.P139S
Spectinomycin
Plate 2 / E / 11 pDONR223_AKT1_p.R370C
Spectinomycin
Plate 2 / E / 12 pDONR223_DCAF4L2_p.P364L
Spectinomycin
Plate 2 / F / 1 pDONR223_CNBD1_p.L396P
Spectinomycin
Plate 2 / F / 2 pDONR223_GABRA6_p.P345S
Spectinomycin
Plate 2 / F / 3 pDONR223_CNBD1_p.Y200F
Spectinomycin
Plate 2 / F / 4 pDONR223_DCAF4L2_p.G100V
Spectinomycin
Plate 2 / F / 5 pDONR223_CNBD1_p.F343C
Spectinomycin
Plate 2 / F / 6 pDONR223_GABRA6_p.R238K
Spectinomycin
Plate 2 / F / 7 pDONR223_DCAF4L2_p.V332L
Spectinomycin
Plate 2 / F / 8 pDONR223_ADORA2A_p.R220W
Spectinomycin
Plate 2 / F / 9 pDONR223_CNBD1_p.A426S
Spectinomycin
Plate 2 / F / 10 pDONR223_IRF4_p.K123R
Spectinomycin
Plate 2 / F / 11 pDONR223_GABRA6_p.G175R
Spectinomycin
Plate 2 / F / 12 pDONR223_JMJD5_p.A230T
Spectinomycin
Plate 2 / G / 1 pDONR223_SGK1_p.A48G
Spectinomycin
Plate 2 / G / 2 pDONR223_CNBD1_p.S372N
Spectinomycin
Plate 2 / G / 3 pDONR223_IDH2_p.R172K
Spectinomycin
Plate 2 / G / 4 pDONR223_KRT26_p.S2C
Spectinomycin
Plate 2 / G / 5 pDONR223_COPS2_p.A317D
Spectinomycin
Plate 2 / G / 6 pDONR223_IRF4_p.R96L
Spectinomycin
Plate 2 / G / 7 pDONR223_CYP3A5_p.G177R
Spectinomycin
Plate 2 / G / 8 pDONR223_SMAD4_p.R97H
Spectinomycin
Plate 2 / G / 9 pDONR223_ACVR1B_p.D335N
Spectinomycin
Plate 2 / G / 10 pDONR223_TBL1XR1_p.L81V
Spectinomycin
Plate 2 / G / 11 pDONR223_PPP2R1A_p.S143F
Spectinomycin
Plate 2 / G / 12 pDONR223_OMA1_p.P177S
Spectinomycin
Plate 2 / H / 1 pDONR223_COPS2_p.G68R
Spectinomycin
Plate 2 / H / 2 pDONR223_IDH2_p.A416V
Spectinomycin
Plate 2 / H / 3 pDONR223_KIR3DL2_p.R96Q
Spectinomycin
Plate 2 / H / 4 pDONR223_ZNF454_p.H462Y
Spectinomycin
Plate 2 / H / 5 pDONR223_SMAD4_p.R361P
Spectinomycin
Plate 2 / H / 6 pDONR223_ACVR1B_p.E412D
Spectinomycin
Plate 2 / H / 7 pDONR223_EYS_p.Q172L
Spectinomycin
Plate 2 / H / 8 pDONR223_GALNTL5_p.G309E
Spectinomycin
Plate 2 / H / 9 pDONR223_IDH2_p.T331M
Spectinomycin
Plate 2 / H / 10 pDONR223_FAM196A_p.Q46R
Spectinomycin
Plate 2 / H / 11 pDONR223_SMAD4_p.D537Y
Spectinomycin
Plate 2 / H / 12 pDONR223_AKT1_p.V201I
Spectinomycin
Plate 3 / A / 1 pDONR223_ZNF454_p.P299L
Spectinomycin
Plate 3 / A / 2 pDONR223_IDH2_p.A47V
Spectinomycin
Plate 3 / A / 3 pDONR223_AKT1_p.D44N
Spectinomycin
Plate 3 / A / 4 pDONR223_KPNA3_p.V154G
Spectinomycin
Plate 3 / A / 5 pDONR223_PIK3R3_p.R383C
Spectinomycin
Plate 3 / A / 6 pDONR223_AKT1_p.E17K
Spectinomycin
Plate 3 / A / 7 pDONR223_TRAF3_p.D463N
Spectinomycin
Plate 3 / A / 8 pDONR223_SLC2A3_p.K49N
Spectinomycin
Plate 3 / A / 9 pDONR223_ZNF454_p.R376I
Spectinomycin
Plate 3 / A / 10 pDONR223_CHD2_p.P195L
Spectinomycin
Plate 3 / A / 11 pDONR223_CDK17_p.R177H
Spectinomycin
Plate 3 / A / 12 pDONR223_GATA3_p.S118F
Spectinomycin
Plate 3 / B / 1 pDONR223_TRIM55_p.E369G
Spectinomycin
Plate 3 / B / 2 pDONR223_CHD2_p.E287Q
Spectinomycin
Plate 3 / B / 3 pDONR223_GALNT14_p.V534F
Spectinomycin
Plate 3 / B / 4 pDONR223_CHD2_p.E412K
Spectinomycin
Plate 3 / B / 5 pDONR223_SPAG8_p.P344L
Spectinomycin
Plate 3 / B / 6 pDONR223_CDK17_p.M85I
Spectinomycin
Plate 3 / B / 7 pDONR223_GABRA6_p.G212R
Spectinomycin
Plate 3 / B / 8 pDONR223_SLC2A3_p.F88S
Spectinomycin
Plate 3 / B / 9 pDONR223_CHD2_p.P474R
Spectinomycin
Plate 3 / B / 10 pDONR223_GALNT14_p.A187T
Spectinomycin
Plate 3 / B / 11 pDONR223_ACVR2A_p.A381V
Spectinomycin
Plate 3 / B / 12 pDONR223_MAP2K5_p.P111S
Spectinomycin
Plate 3 / C / 1 pDONR223_SLC2A3_p.G109V
Spectinomycin
Plate 3 / C / 2 pDONR223_AKT1_p.Q79K
Spectinomycin
Plate 3 / C / 3 pDONR223_CYP3A5_p.D336N
Spectinomycin
Plate 3 / C / 4 pDONR223_ARSI_p.V373M
Spectinomycin
Plate 3 / C / 5 pDONR223_TBL1XR1_p.T349M
Spectinomycin
Plate 3 / C / 6 pDONR223_TBL1XR1_p.R166Q
Spectinomycin
Plate 3 / C / 7 pDONR223_SMAD4_p.K519N
Spectinomycin
Plate 3 / C / 8 pDONR223_EYS_p.R587I
Spectinomycin
Plate 3 / C / 9 pDONR223_ELF1_p.P32S
Spectinomycin
Plate 3 / C / 10 pDONR223_KIF2B_p.I119T
Spectinomycin
Plate 3 / C / 11 pDONR223_PTPN12_p.G99R
Spectinomycin
Plate 3 / C / 12 pDONR223_GALNT14_p.P484S
Spectinomycin
Plate 3 / D / 1 pDONR223_EYS_p.C554S
Spectinomycin
Plate 3 / D / 2 pDONR223_KEAP1_p.G603W
Spectinomycin
Plate 3 / D / 3 pDONR223_FBXW7_p.A502V
Spectinomycin
Plate 3 / D / 4 pDONR223_FAM200A_p.S481N
Spectinomycin
Plate 3 / D / 5 pDONR223_KEAP1_p.S45F
Spectinomycin
Plate 3 / D / 6 pDONR223_POT1_p.G76V
Spectinomycin
Plate 3 / D / 7 pDONR223_KIF2B_p.E161G
Spectinomycin
Plate 3 / D / 8 pDONR223_FBXW7_p.R465C
Spectinomycin
Plate 3 / D / 9 pDONR223_THEMIS_p.E503K
Spectinomycin
Plate 3 / D / 10 pDONR223_KIF2B_p.R504Q
Spectinomycin
Plate 3 / D / 11 pDONR223_FBXW7_p.R658Q
Spectinomycin
Plate 3 / D / 12 pDONR223_SMAD4_p.E134K
Spectinomycin
Plate 3 / E / 1 pDONR223_WBSCR17_p.S194R
Spectinomycin
Plate 3 / E / 2 pDONR223_KEAP1_p.G419W
Spectinomycin
Plate 3 / E / 3 pDONR223_SAMHD1_p.I515T
Spectinomycin
Plate 3 / E / 4 pDONR223_FBXW7_p.R505C
Spectinomycin
Plate 3 / E / 5 pDONR223_THEMIS_p.P216S
Spectinomycin
Plate 3 / E / 6 pDONR223_KIF2B_p.W60L
Spectinomycin
Plate 3 / E / 7 pDONR223_SMAD4_p.R361H
Spectinomycin
Plate 3 / E / 8 pDONR223_NFE2L2_p.T80K
Spectinomycin
Plate 3 / E / 9 pDONR223_WBSCR17_p.A398V
Spectinomycin
Plate 3 / E / 10 pDONR223_SAMHD1_p.D218H
Spectinomycin
Plate 3 / E / 11 pDONR223_CTCF_p.P552S
Spectinomycin
Plate 3 / E / 12 pDONR223_THEMIS_p.R196I
Spectinomycin
Plate 3 / F / 1 pDONR223_FBXW7_p.R689Q
Spectinomycin
Plate 3 / F / 2 pDONR223_EYS_p.H466N
Spectinomycin
Plate 3 / F / 3 pDONR223_NFE2L2_p.G31V
Spectinomycin
Plate 3 / F / 4 pDONR223_KEAP1_p.E611D
Spectinomycin
Plate 3 / F / 5 pDONR223_NFE2L2_p.G31R
Spectinomycin
Plate 3 / F / 6 pDONR223_LNX1_p.V564I
Spectinomycin
Plate 3 / F / 7 pDONR223_SCEL_p.S10Y
Spectinomycin
Plate 3 / F / 8 pDONR223_EYS_p.R26Q
Spectinomycin
Plate 3 / F / 9 pDONR223_NFE2L2_p.G31A
Spectinomycin
Plate 3 / F / 10 pDONR223_KEAP1_p.T142M
Spectinomycin
Plate 3 / F / 11 pDONR223_BAP1_p.D75G
Spectinomycin
Plate 3 / F / 12 pDONR223_PTPN12_p.E57D
Spectinomycin
Plate 3 / G / 1 pDONR223_RASA1_p.R913Q
Spectinomycin
Plate 3 / G / 2 pDONR223_SV2C_p.G146S
Spectinomycin
Plate 3 / G / 3 pDONR223_PTPN12_p.E5G
Spectinomycin
Plate 3 / G / 4 pDONR223_PIK3CB_p.A1048V
Spectinomycin
Plate 3 / G / 5 pDONR223_SYCP1_p.E773K
Spectinomycin
Plate 3 / G / 6 pDONR223_SV2C_p.G630S
Spectinomycin
Plate 3 / G / 7 pDONR223_STAT6_p.D46N
Spectinomycin
Plate 3 / G / 8 pDONR223_SYCP1_p.D889N
Spectinomycin
Plate 3 / G / 9 pDONR223_PIK3R4_p.E1209K
Spectinomycin
Plate 3 / G / 10 pDONR223_SV2C_p.M727I
Spectinomycin
Plate 3 / G / 11 pDONR223_GEN1_p.L235R
Spectinomycin
Plate 3 / G / 12 pDONR223_PIK3CG_p.V168M
Spectinomycin
Plate 3 / H / 1 pDONR223_SV2C_p.R121Q
Spectinomycin
Plate 3 / H / 2 pDONR223_DNMT3A_p.G746V
Spectinomycin
Plate 3 / H / 3 pDONR223_RB1_p.F755I
Spectinomycin
Plate 3 / H / 4 pDONR223_RB1_p.I782M
Spectinomycin
Plate 3 / H / 5 pDONR223_GPR156_p.R757L
Spectinomycin
Plate 3 / H / 6 pDONR223_GPR156_p.C763S
Spectinomycin
Plate 3 / H / 7 pDONR223_GALNT14_p.F285L
Spectinomycin
Plate 3 / H / 8 pDONR223_YTHDC1_p.E483Q
Spectinomycin
Plate 3 / H / 9 pDONR223_THEMIS_p.D240N
Spectinomycin
Plate 3 / H / 10 pDONR223_XPO1_p.E571K
Spectinomycin
Plate 3 / H / 11 pDONR223_XPO1_p.R553C
Spectinomycin
Plate 3 / H / 12 pDONR223_EYS_p.N269H
Spectinomycin
Plate 4 / A / 1 pDONR223_NFE2L2_p.N160S
Spectinomycin
Plate 4 / A / 2 pDONR223_POT1_p.L265H
Spectinomycin
Plate 4 / A / 3 pDONR223_THEMIS_p.H366Q
Spectinomycin
Plate 4 / A / 4 pDONR223_AKT1_p.E267G
Spectinomycin
Plate 4 / A / 5 pDONR223_RB1_p.V654L
Spectinomycin
Plate 4 / A / 6 pDONR223_GALNTL5_p.D221E
Spectinomycin
Plate 4 / A / 7 pDONR223_KIF2B_p.E211K
Spectinomycin
Plate 4 / A / 8 pDONR223_KIF2B_p.Q430H
Spectinomycin
Plate 4 / A / 9 pDONR223_CTCF_p.R377H
Spectinomycin
Plate 4 / A / 10 pDONR223_KEAP1_p.P318L
Spectinomycin
Plate 4 / A / 11 pDONR223_ERBB2IP_p.I979M
Spectinomycin
Plate 4 / A / 12 pDONR223_ARSI_p.P228L
Spectinomycin
Plate 4 / B / 1 pDONR223_POT1_p.L388M
Spectinomycin
Plate 4 / B / 2 pDONR223_FBXW7_p.R465H
Spectinomycin
Plate 4 / B / 3 pDONR223_BAP1_p.N299S
Spectinomycin
Plate 4 / B / 4 pDONR223_RASA1_p.R194C
Spectinomycin
Plate 4 / B / 5 pDONR223_TRAF3_p.N312S
Spectinomycin
Plate 4 / B / 6 pDONR223_WBSCR17_p.E289K
Spectinomycin
Plate 4 / B / 7 pDONR223_FBXW7_p.I347M
Spectinomycin
Plate 4 / B / 8 pDONR223_GALNT14_p.R336Q
Spectinomycin
Plate 4 / B / 9 pDONR223_STAG2_p.R541K
Spectinomycin
Plate 4 / B / 10 pDONR223_WBSCR17_p.D348N
Spectinomycin
Plate 4 / B / 11 pDONR223_PPP2R1A_p.R258H
Spectinomycin
Plate 4 / B / 12 pDONR223_EYS_p.F336L
Spectinomycin
Plate 4 / C / 1 pDONR223_FBXW7_p.S462Y
Spectinomycin
Plate 4 / C / 2 pDONR223_CTCF_p.R377C
Spectinomycin
Plate 4 / C / 3 pDONR223_SYCP1_p.E442K
Spectinomycin
Plate 4 / C / 4 pDONR223_SYCP1_p.E509K
Spectinomycin
Plate 4 / C / 5 pDONR223_ERBB2IP_p.E463K
Spectinomycin
Plate 4 / C / 6 pDONR223_STAG2_p.L997W
Spectinomycin
Plate 4 / C / 7 pDONR223_STAG2_p.R252Q
Spectinomycin
Plate 4 / C / 8 pDONR223_PIK3R4_p.N870T
Spectinomycin
Plate 4 / C / 9 pDONR223_STAT6_p.N417Y
Spectinomycin
Plate 4 / C / 10 pDONR223_RBM10_p.R171W
Spectinomycin
Plate 4 / C / 11 pDONR223_PIK3R4_p.R998Q
Spectinomycin
Plate 4 / C / 12 pDONR223_CHSY3_p.R350H
Spectinomycin
Plate 4 / D / 1 pDONR223_RB1_p.R451H
Spectinomycin
Plate 4 / D / 2 pDONR223_RB1_p.L550I
Spectinomycin
Plate 4 / D / 3 pDONR223_DNMT3A_p.Q485H
Spectinomycin
Plate 4 / D / 4 pDONR223_STAG2_p.P572S
Spectinomycin
Plate 4 / D / 5 pDONR223_PIK3CB_p.E497D
Spectinomycin
Plate 4 / D / 6 pDONR223_XPO1_p.E571A
Spectinomycin
Plate 4 / D / 7 pDONR223_RASA1_p.E510K
Spectinomycin
Plate 4 / D / 8 pDONR223_PIK3CB_p.A593V
Spectinomycin
Plate 4 / D / 9 pDONR223_CD70_p.D165Y
Spectinomycin
Plate 4 / D / 10 pDONR223_AKT1_p.L52R
Spectinomycin
Plate 4 / D / 11 pDONR223_GABRA6_p.A322T
Spectinomycin
Plate 4 / D / 12 pDONR223_RASA1_p.L146S
Spectinomycin
Plate 4 / E / 1 pDONR223_DNMT3A_p.A910V
Spectinomycin
Plate 4 / E / 2 pDONR223_CYLC2_p.D299Y
Spectinomycin
Plate 4 / E / 3 pDONR223_TRIM55_p.R322H
Spectinomycin
Plate 4 / E / 4 pDONR223_DCAF4L2_p.R172C
Spectinomycin
Plate 4 / E / 5 pDONR223_GATA3_p.S140F
Spectinomycin
Plate 4 / E / 6 pDONR223_CTCF_p.N568K
Spectinomycin
Plate 4 / E / 7 pDONR223_LCE1B_p.G102C
Spectinomycin
Plate 4 / E / 8 pDONR223_SERPINB4_p.E250K
Spectinomycin
Plate 4 / E / 9 pDONR223_SMAD2_p.D304G
Spectinomycin
Plate 4 / E / 10 pDONR223_TMPO_p.D185H
Spectinomycin
Plate 4 / E / 11 pDONR223_TUBB_p.R359P
Spectinomycin
Plate 4 / E / 12 pDONR223_IDH2_p.R172M
Spectinomycin
Plate 4 / F / 1 pDONR223_WBSCR17_p.E507K
Spectinomycin
Plate 4 / F / 2 pDONR223_KEAP1_p.R470H
Spectinomycin
Plate 4 / F / 3 pDONR223_SLC46A1_p.W299S
Spectinomycin
Plate 4 / F / 4 pDONR223_PEX3_p.G173E
Spectinomycin
Plate 4 / F / 5 pDONR223_MAGEC2_p.D15N
Spectinomycin
Plate 4 / F / 6 pDONR223_HIST1H3B_p.L101W
Spectinomycin
Plate 4 / F / 7 pDONR223_FAM116A_p.R427H
Spectinomycin
Plate 4 / F / 8 pDONR223_IDH2_p.I139F
Spectinomycin
Plate 4 / F / 9 pDONR223_PIK3CG_p.K973R
Spectinomycin
Plate 4 / F / 10 pDONR223_STAG2_p.E134D
Spectinomycin
Plate 4 / F / 11 pDONR223_PIK3CG_p.W212C
Spectinomycin
Plate 4 / F / 12 pDONR223_SPOP_p.E50K
Spectinomycin
Plate 4 / G / 1 pDONR223_SPOP_p.W131G
Spectinomycin
Plate 4 / G / 2 pDONR223_SNURF_p.M1I
Spectinomycin
Plate 4 / G / 3 pDONR223_OR2M2_p.E232V
Spectinomycin
Plate 4 / G / 4 pDONR223_OR2M2_p.P182S
Spectinomycin
Plate 4 / G / 5 pDONR223_ASCL4_p.T114M
Spectinomycin
Plate 4 / G / 6 pDONR223_SPOP_p.F133S
Spectinomycin
Plate 4 / G / 7 pDONR223_OR2M2_p.N42Y
Spectinomycin
Plate 4 / G / 8 pDONR223_OR2M2_p.V117I
Spectinomycin
Plate 4 / G / 9 pDONR223_HAPLN1_p.S326N
Spectinomycin
Plate 4 / G / 10 pDONR223_SPOP_p.K101I
Spectinomycin
Plate 4 / G / 11 pDONR223_SPOP_p.F102C
Spectinomycin
Plate 4 / G / 12 pDONR223_SPOP_p.W131C
Spectinomycin
Plate 4 / H / 1 pDONR223_SPOP_p.E47A
Spectinomycin
Plate 4 / H / 2 pDONR223_HAPLN1_p.K115N
Spectinomycin
Plate 4 / H / 3 pDONR223_SPOP_p.K134N
Spectinomycin
Plate 4 / H / 4 pDONR223_HAPLN1_p.P233H
Spectinomycin
Plate 4 / H / 5 pDONR223_STK19_p.E88K
Spectinomycin
Plate 4 / H / 6 pDONR223_PTEN_p.G127R
Spectinomycin
Plate 4 / H / 7 pDONR223_KRAS_p.G12D
Spectinomycin
Plate 4 / H / 8 pDONR223_NRAS_p.Q61R
Spectinomycin
Plate 4 / H / 9 pDONR223_VHL_p.V130F
Spectinomycin
Plate 4 / H / 10 pDONR223_KRAS_p.G12S
Spectinomycin
Plate 4 / H / 11 pDONR223_PTEN_p.R233Q
Spectinomycin
Plate 4 / H / 12 pDONR223_KRAS_p.E62K
Spectinomycin
Plate 5 / A / 1 pDONR223_NRAS_p.Q61K
Spectinomycin
Plate 5 / A / 2 pDONR223_VHL_p.H115Y
Spectinomycin
Plate 5 / A / 3 pDONR223_KRAS_p.D33E
Spectinomycin
Plate 5 / A / 4 pDONR223_NRAS_p.G12C
Spectinomycin
Plate 5 / A / 5 pDONR223_VHL_p.E186K
Spectinomycin
Plate 5 / A / 6 pDONR223_KRAS_p.A59G
Spectinomycin
Plate 5 / A / 7 pDONR223_HTR4_p.Y129H
Spectinomycin
Plate 5 / A / 8 pDONR223_NRAS_p.G12A
Spectinomycin
Plate 5 / A / 9 pDONR223_KRAS_p.G12V
Spectinomycin
Plate 5 / A / 10 pDONR223_NRAS_p.Y64D
Spectinomycin
Plate 5 / A / 11 pDONR223_KRAS_p.G12C
Spectinomycin
Plate 5 / A / 12 pDONR223_VHL_p.L158V
Spectinomycin
Plate 5 / B / 1 pDONR223_MAP2K1_p.C121S
Spectinomycin
Plate 5 / B / 2 pDONR223_NRAS_p.Q61H
Spectinomycin
Plate 5 / B / 3 pDONR223_VHL_p.R161P
Spectinomycin
Plate 5 / B / 4 pDONR223_MAP2K1_p.L177M
Spectinomycin
Plate 5 / B / 5 pDONR223_KRAS_p.G12A
Spectinomycin
Plate 5 / B / 6 pDONR223_NRAS_p.Q61L
Spectinomycin
Plate 5 / B / 7 pDONR223_VHL_p.S111N
Spectinomycin
Plate 5 / B / 8 pDONR223_IDH1_p.P33S
Spectinomycin
Plate 5 / B / 9 pDONR223_CTNNB1_p.I35S
Spectinomycin
Plate 5 / B / 10 pDONR223_MTM1_p.P226S
Spectinomycin
Plate 5 / B / 11 pDONR223_IDH1_p.R132L
Spectinomycin
Plate 5 / B / 12 pDONR223_MAP2K4_p.S184L
Spectinomycin
Plate 5 / C / 1 pDONR223_PTEN_p.R173H
Spectinomycin
Plate 5 / C / 2 pDONR223_PTEN_p.G127V
Spectinomycin
Plate 5 / C / 3 pDONR223_BRAF_p.L597Q
Spectinomycin
Plate 5 / C / 4 pDONR223_PTEN_p.G129V
Spectinomycin
Plate 5 / C / 5 pDONR223_IDH1_p.R132H
Spectinomycin
Plate 5 / C / 6 pDONR223_BRAF_p.N581S
Spectinomycin
Plate 5 / C / 7 pDONR223_MAP4K3_p.R753Q
Spectinomycin
Plate 5 / C / 8 pDONR223_PTEN_p.G129E
Spectinomycin
Plate 5 / C / 9 pDONR223_CTNNB1_p.H36Y
Spectinomycin
Plate 5 / C / 10 pDONR223_WNK3_p.T237M
Spectinomycin
Plate 5 / C / 11 pDONR223_PTEN_p.F90S
Spectinomycin
Plate 5 / C / 12 pDONR223_CTNNB1_p.G34V
Spectinomycin
Plate 5 / D / 1 pDONR223_MAP2K1_p.K57T
Spectinomycin
Plate 5 / D / 2 pDONR223_HTR3B_p.W95C
Spectinomycin
Plate 5 / D / 3 pDONR223_CTNNB1_p.G34R
Spectinomycin
Plate 5 / D / 4 pDONR223_PTEN_p.K6N
Spectinomycin
Plate 5 / D / 5 pDONR223_MEF2C_p.T9M
Spectinomycin
Plate 5 / D / 6 pDONR223_BRAF_p.V600E
Spectinomycin
Plate 5 / D / 7 pDONR223_PIK3CA_p.H1047Y
Spectinomycin
Plate 5 / D / 8 pDONR223_PIK3CA_p.D1045V
Spectinomycin
Plate 5 / D / 9 pDONR223_MAP2K1_p.P124S
Spectinomycin
Plate 5 / D / 10 pDONR223_IDH1_p.E190K
Spectinomycin
Plate 5 / D / 11 pDONR223_ERBB2_p.T182I
Spectinomycin
Plate 5 / D / 12 pDONR223_MAP4K3_p.Q473H
Spectinomycin
Plate 5 / E / 1 pDONR223_BRAF_p.G466A
Spectinomycin
Plate 5 / E / 2 pDONR223_EGFR_p.G719D
Spectinomycin
Plate 5 / E / 3 pDONR223_WNK3_p.S617F
Spectinomycin
Plate 5 / E / 4 pDONR223_BRAF_p.G466V
Spectinomycin
Plate 5 / E / 5 pDONR223_MTM1_p.D298E
Spectinomycin
Plate 5 / E / 6 pDONR223_MAPK6_p.D387N
Spectinomycin
Plate 5 / E / 7 pDONR223_CTNNB1_p.R535Q
Spectinomycin
Plate 5 / E / 8 pDONR223_ERBB2_p.R897Q
Spectinomycin
Plate 5 / E / 9 pDONR223_BRAF_p.G466E
Spectinomycin
Plate 5 / E / 10 pDONR223_WNK3_p.E417Q
Spectinomycin
Plate 5 / E / 11 pDONR223_CTNNB1_p.T257I
Spectinomycin
Plate 5 / E / 12 pDONR223_EGFR_p.P622S
Spectinomycin
Plate 5 / F / 1 pDONR223_ERBB2_p.V842I
Spectinomycin
Plate 5 / F / 2 pDONR223_WNK3_p.V488A
Spectinomycin
Plate 5 / F / 3 pDONR223_MTM1_p.R253Q
Spectinomycin
Plate 5 / F / 4 pDONR223_IDH1_p.R132C
Spectinomycin
Plate 5 / F / 5 pDONR223_CTNNB1_p.G34E
Spectinomycin
Plate 5 / F / 6 pDONR223_MAP2K1_p.E203K
Spectinomycin
Plate 5 / F / 7 pDONR223_HTR3B_p.I118T
Spectinomycin
Plate 5 / F / 8 pDONR223_HTR4_p.T105M
Spectinomycin
Plate 5 / F / 9 pDONR223_CTNNB1_p.D162E
Spectinomycin
Plate 5 / F / 10 pDONR223_EGFR_p.A289V
Spectinomycin
Plate 6 / A / 1 pDONR223_CTNNB1_WT
Spectinomycin
Plate 6 / A / 2 pDONR223_MEF2C_WT
Spectinomycin
Plate 6 / A / 3 pDONR223_PIK3CA_WT
Spectinomycin
Plate 6 / A / 4 pDONR223_PTEN_WT
Spectinomycin
Plate 6 / A / 5 pDONR223_ACVR1B_WT
Spectinomycin
Plate 6 / A / 6 pDONR223_RHOA_WT
Spectinomycin
Plate 6 / A / 7 pDONR223_PRDM1_WT
Spectinomycin
Plate 6 / A / 8 pDONR223_CDK4_WT
Spectinomycin
Plate 6 / A / 9 pDONR223_EZH2_WT
Spectinomycin
Plate 6 / A / 10 pDONR223_SMARCB1_WT
Spectinomycin
Plate 6 / A / 11 pDONR223_STK11_WT
Spectinomycin
Plate 6 / A / 12 pDONR223_TCF7L2_WT
Spectinomycin
Plate 6 / B / 1 pDONR223_BCL6_WT
Spectinomycin
Plate 6 / B / 2 pDONR223_IRF4_WT
Spectinomycin
Plate 6 / B / 3 pDONR223_PIK3R1_WT
Spectinomycin
Plate 6 / B / 4 pDONR223_RAC1_WT
Spectinomycin
Plate 6 / B / 5 pDONR223_CD79B_WT
Spectinomycin
Plate 6 / B / 6 pDONR223_KRAS_WT
Spectinomycin
Plate 6 / B / 7 pDONR223_TP53_WT
Spectinomycin
Plate 6 / B / 8 pDONR223_TRAF3_WT
Spectinomycin
Plate 6 / B / 9 pDONR223_JAK2_WT
Spectinomycin
Plate 6 / B / 10 pDONR223_HAPLN1_WT
Spectinomycin
Plate 6 / B / 11 pDONR223_OR2M2_WT
Spectinomycin
Plate 6 / B / 12 pDONR223_PPP2R1A_WT
Spectinomycin
Plate 6 / C / 1 pDONR223_HIST1H1E_WT
Spectinomycin
Plate 6 / C / 2 pDONR223_KRT26_WT
Spectinomycin
Plate 6 / C / 3 pDONR223_AKT1_WT
Spectinomycin
Plate 6 / C / 4 pDONR223_DNMT3A_WT
Spectinomycin
Plate 6 / C / 5 pDONR223_SERPINB4_WT
Spectinomycin
Plate 6 / C / 6 pDONR223_CCNG1_WT
Spectinomycin
Plate 6 / C / 7 pDONR223_CHD2_WT
Spectinomycin
Plate 6 / C / 8 pDONR223_NFE2L2_WT
Spectinomycin
Plate 6 / C / 9 pDONR223_RBM10_WT
Spectinomycin
Plate 6 / C / 10 pDONR223_KIR3DL2_WT
Spectinomycin
Plate 6 / C / 11 pDONR223_ATPIF1_WT
Spectinomycin
Plate 6 / C / 12 pDONR223_WNT2_WT
Spectinomycin
Plate 6 / D / 1 pDONR223_CDK17_WT
Spectinomycin
Plate 6 / D / 2 pDONR223_HRAS_WT
Spectinomycin
Plate 6 / D / 3 pDONR223_RASA1_WT
Spectinomycin
Plate 6 / D / 4 pDONR223_PEX3_WT
Spectinomycin
Plate 6 / D / 5 pDONR223_GLIPR1_WT
Spectinomycin
Plate 6 / D / 6 pDONR223_RBM11_WT
Spectinomycin
Plate 6 / D / 7 pDONR223_REG1A_WT
Spectinomycin
Plate 6 / D / 8 pDONR223_HRH4_WT
Spectinomycin
Plate 6 / D / 9 pDONR223_SLC2A3_WT
Spectinomycin
Plate 6 / D / 10 pDONR223_CTCF_WT
Spectinomycin
Plate 6 / D / 11 pDONR223_HAUS1_WT
Spectinomycin
Plate 6 / D / 12 pDONR223_GZMA_WT
Spectinomycin
Plate 6 / E / 1 pDONR223_COPS2_WT
Spectinomycin
Plate 6 / E / 2 pDONR223_C19orf48_WT
Spectinomycin
Plate 6 / E / 3 pDONR223_FBXW7_WT
Spectinomycin
Plate 6 / E / 4 pDONR223_RAB42_WT
Spectinomycin
Plate 6 / E / 5 pDONR223_ERO1L_WT
Spectinomycin
Plate 6 / E / 6 pDONR223_YTHDC1_WT
Spectinomycin
Plate 6 / E / 7 pDONR223_IDH2_WT
Spectinomycin
Plate 6 / E / 8 pDONR223_PNKP_WT
Spectinomycin
Plate 6 / E / 9 pDONR223_HIST1H3B_WT
Spectinomycin
Plate 6 / E / 10 pDONR223_RIMS2_WT
Spectinomycin
Plate 6 / E / 11 pDONR223_LNX1_WT
Spectinomycin
Plate 6 / E / 12 pDONR223_C20orf72_WT
Spectinomycin
Plate 6 / F / 1 pDONR223_U2AF1_WT
Spectinomycin
Plate 6 / F / 2 pDONR223_ILKAP_WT
Spectinomycin
Plate 6 / F / 3 pDONR223_B2M_WT
Spectinomycin
Plate 6 / F / 4 pDONR223_PPP6C_WT
Spectinomycin
Plate 6 / F / 5 pDONR223_XPO1_WT
Spectinomycin
Plate 6 / F / 6 pDONR223_OTUD6A_WT
Spectinomycin
Plate 6 / F / 7 pDONR223_ROGDI_WT
Spectinomycin
Plate 6 / F / 8 pDONR223_DNASE2_WT
Spectinomycin
Plate 6 / F / 9 pDONR223_GPS2_WT
Spectinomycin
Plate 6 / F / 10 pDONR223_DCAF4L2_WT
Spectinomycin
Plate 6 / F / 11 pDONR223_TMPO_WT
Spectinomycin
Plate 6 / F / 12 pDONR223_CPXCR1_WT
Spectinomycin
Plate 6 / G / 1 pDONR223_POT1_WT
Spectinomycin
Plate 6 / G / 2 pDONR223_GPR156_WT
Spectinomycin
Plate 6 / G / 3 pDONR223_CYP3A5_WT
Spectinomycin
Plate 6 / G / 4 pDONR223_TNF_WT
Spectinomycin
Plate 6 / G / 5 pDONR223_FGF1_WT
Spectinomycin
Plate 6 / G / 6 pDONR223_REG3A_WT
Spectinomycin
Plate 6 / G / 7 pDONR223_SYCP1_WT
Spectinomycin
Plate 6 / G / 8 pDONR223_TRIM55_WT
Spectinomycin
Plate 6 / G / 9 pDONR223_SMAD2_WT
Spectinomycin
Plate 6 / G / 10 pDONR223_ZDHHC4_WT
Spectinomycin
Plate 6 / G / 11 pDONR223_NFKBIA_WT
Spectinomycin
Plate 6 / G / 12 pDONR223_SGK1_WT
Spectinomycin
Plate 6 / H / 1 pDONR223_A4GALT_WT
Spectinomycin
Plate 6 / H / 2 pDONR223_CDKN1B_WT
Spectinomycin
Plate 6 / H / 3 pDONR223_WBSCR17_WT
Spectinomycin
Plate 6 / H / 4 pDONR223_FBXO15_WT
Spectinomycin
Plate 6 / H / 5 pDONR223_ACVR2A_WT
Spectinomycin
Plate 6 / H / 6 pDONR223_LCE1B_WT
Spectinomycin
Plate 6 / H / 7 pDONR223_C1orf150_WT
Spectinomycin
Plate 6 / H / 8 pDONR223_PIK3CG_WT
Spectinomycin
Plate 6 / H / 9 pDONR223_GEN1_WT
Spectinomycin
Plate 6 / H / 10 pDONR223_RGR_WT
Spectinomycin
Plate 6 / H / 11 pDONR223_OR2G6_WT
Spectinomycin
Plate 6 / H / 12 pDONR223_SPAG8_WT
Spectinomycin
Plate 7 / A / 1 pDONR223_ISX_WT
Spectinomycin
Plate 7 / A / 2 pDONR223_TNFRSF14_WT
Spectinomycin
Plate 7 / A / 3 pDONR223_OR2T33_WT
Spectinomycin
Plate 7 / A / 4 pDONR223_BAP1_WT
Spectinomycin
Plate 7 / A / 5 pDONR223_PIK3IP1_WT
Spectinomycin
Plate 7 / A / 6 pDONR223_EYS_WT
Spectinomycin
Plate 7 / A / 7 pDONR223_IKZF5_WT
Spectinomycin
Plate 7 / A / 8 pDONR223_SPOP_WT
Spectinomycin
Plate 7 / A / 9 pDONR223_RB1_WT
Spectinomycin
Plate 7 / A / 10 pDONR223_MFAP4_WT
Spectinomycin
Plate 7 / A / 11 pDONR223_PIK3R4_WT
Spectinomycin
Plate 7 / A / 12 pDONR223_RNF212_WT
Spectinomycin
Plate 7 / B / 1 pDONR223_GABRA6_WT
Spectinomycin
Plate 7 / B / 2 pDONR223_MAGEC2_WT
Spectinomycin
Plate 7 / B / 3 pDONR223_FAM116A_WT
Spectinomycin
Plate 7 / B / 4 pDONR223_ZNF454_WT
Spectinomycin
Plate 7 / B / 5 pDONR223_CD3E_WT
Spectinomycin
Plate 7 / B / 6 pDONR223_SPATA8_WT
Spectinomycin
Plate 7 / B / 7 pDONR223_TPTE_WT
Spectinomycin
Plate 7 / B / 8 pDONR223_SCEL_WT
Spectinomycin
Plate 7 / B / 9 pDONR223_PRR23B_WT
Spectinomycin
Plate 7 / B / 10 pDONR223_C13orf37_WT
Spectinomycin
Plate 7 / B / 11 pDONR223_POU2F2_WT
Spectinomycin
Plate 7 / B / 12 pDONR223_VHL_WT
Spectinomycin
Plate 7 / C / 1 pDONR223_GPR141_WT
Spectinomycin
Plate 7 / C / 2 pDONR223_CNBD1_WT
Spectinomycin
Plate 7 / C / 3 pDONR223_FAM200A_WT
Spectinomycin
Plate 7 / C / 4 pDONR223_FTH1_WT
Spectinomycin
Plate 7 / C / 5 pDONR223_HERPUD2_WT
Spectinomycin
Plate 7 / C / 6 pDONR223_EDNRB_WT
Spectinomycin
Plate 7 / C / 7 pDONR223_FAM196A_WT
Spectinomycin
Plate 7 / C / 8 pDONR223_ELF1_WT
Spectinomycin
Plate 7 / C / 9 pDONR223_CPZ_WT
Spectinomycin
Plate 7 / C / 10 pDONR223_NMNAT3_WT
Spectinomycin
Plate 7 / C / 11 pDONR223_KLRC2_WT
Spectinomycin
Plate 7 / C / 12 pDONR223_SMR3A_WT
Spectinomycin
Plate 7 / D / 1 pDONR223_ADORA2A_WT
Spectinomycin
Plate 7 / D / 2 pDONR223_OMA1_WT
Spectinomycin
Plate 7 / D / 3 pDONR223_THEMIS_WT
Spectinomycin
Plate 7 / D / 4 pDONR223_KPNA3_WT
Spectinomycin
Plate 7 / D / 5 pDONR223_ERBB2_WT
Spectinomycin
Plate 7 / D / 6 pDONR223_WIPI1_WT
Spectinomycin
Plate 7 / D / 7 pDONR223_GALNTL5_WT
Spectinomycin
Plate 7 / D / 8 pDONR223_C15orf23_WT
Spectinomycin
Plate 7 / D / 9 pDONR223_ARSI_WT
Spectinomycin
Plate 7 / D / 10 pDONR223_SAMHD1_WT
Spectinomycin
Plate 7 / D / 11 pDONR223_PPIAL4G_WT
Spectinomycin
Plate 7 / D / 12 pDONR223_NRAS_WT
Spectinomycin
Plate 7 / E / 1 pDONR223_GATA3_WT
Spectinomycin
Plate 7 / E / 2 pDONR223_PIK3R3_WT
Spectinomycin
Plate 7 / E / 3 pDONR223_BTC_WT
Spectinomycin
Plate 7 / E / 4 pDONR223_WISP2_WT
Spectinomycin
Plate 7 / E / 5 pDONR223_MTM1_WT
Spectinomycin
Plate 7 / E / 6 pDONR223_ASCL4_WT
Spectinomycin
Plate 7 / E / 7 pDONR223_ACTG1_WT
Spectinomycin
Plate 7 / E / 8 pDONR223_HTR4_WT
Spectinomycin
Plate 7 / E / 9 pDONR223_PSG2_WT
Spectinomycin
Plate 7 / E / 10 pDONR223_STK19_WT
Spectinomycin
Plate 7 / E / 11 pDONR223_MAP2K1_WT
Spectinomycin
Plate 7 / E / 12 pDONR223_IDH1_WT
Spectinomycin
Plate 7 / F / 1 pDONR223_TRAT1_WT
Spectinomycin
Plate 7 / F / 2 pDONR223_SV2C_WT
Spectinomycin
Plate 7 / F / 3 pDONR223_KEAP1_WT
Spectinomycin
Plate 7 / F / 4 pDONR223_EGFR_WT
Spectinomycin
Plate 7 / F / 5 pDONR223_CBFB_WT
Spectinomycin
Plate 7 / F / 6 pDONR223_H2AFX_WT
Spectinomycin
Plate 7 / F / 7 pDONR223_STAT6_WT
Spectinomycin
Plate 7 / F / 8 pDONR223_CXCR4_WT
Spectinomycin
Plate 7 / F / 9 pDONR223_GNA13_WT
Spectinomycin
Plate 7 / F / 10 pDONR223_STAG2_WT
Spectinomycin
Plate 7 / F / 11 pDONR223_DNAJC12_WT
Spectinomycin
Plate 7 / F / 12 pDONR223_UNC93B1_WT
Spectinomycin
Plate 7 / G / 1 pDONR223_TUBB_WT
Spectinomycin
Plate 7 / G / 2 pDONR223_SPANXN4_WT
Spectinomycin
Plate 7 / G / 3 pDONR223_CD70_WT
Spectinomycin
Plate 7 / G / 4 pDONR223_PDZD11_WT
Spectinomycin
Plate 7 / G / 5 pDONR223_WIPI1_WT
Spectinomycin
Plate 7 / G / 6 pDONR223_TBL1XR1_WT
Spectinomycin
Plate 7 / G / 7 pDONR223_NBPF15_WT
Spectinomycin
Plate 7 / G / 8 pDONR223_ELF3_WT
Spectinomycin
Plate 7 / G / 9 pDONR223_KIF2B_WT
Spectinomycin
Data calculated @ 2017-11-21

Kit Plate Map - #1000000103