Human CRISPR Metabolic Gene Knockout Library
(Pooled Library #110066)

• Purpose:

  This lentiviral CRISPR library targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.

• Vector Backbone:

  lentiCRISPR v1- does express Cas9

  Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages recipient scientists to use the updated lentiCRISPR v2, which can be requested separately.

• Depositing Labs: David Sabatini
• Publication: Birsoy et al. Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.

Library Details

• Species: Human
• Genes targeted: 2,981
• gRNAs: 30,290
• Controls: 500 intergenic
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Transformation Protocol (16 KB)
  • Screening Protocol (17 KB)
  • PCR protocol for Illumina sequencing preparation (15 KB)
• Depositor Data:
  • List of targeted genes (63.5 KB)
  • List of gRNA sequences (1 MB)
  • Distribution of NGS Reads (9 KB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

• Diagram of Library Screening Process

When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).