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Showing: 281 - 300 of 1696 results

  1. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  4. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  10. pCFJ1955

    Plasmid
    #84830
    Purpose
    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized tdTomato
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84830-plasmid-map-sequence-id-163668

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  11. pDD362

    Plasmid
    #137023
    Purpose
    SapTrap HaloTag-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized Halo Tag
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized Halo Tag
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137023-plasmid-map-sequence-id-274041

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  15. pPD93_65

    Plasmid
    #1475
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc54-204, unc545', IVSA, SV40NLS, gfpN, IVS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1475-plasmid-map-sequence-id-86

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  16. pJJR83

    Plasmid
    #75028
    Purpose
    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Article
    Ramalho et al Development. 2020 Jul 22;147(14):dev1880
    Insert
    mCherry^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized mCherry
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75028-plasmid-map-sequence-id-141918

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Showing: 281 - 300 of 1696 results