3,637 results

1. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

   Plasmid

   #172065

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pBP-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199202

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[21-881]-C-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACUH-GFP1-10 mitochondrial matrix

   Plasmid

   #172063

   Purpose

   UAS vector to express GFP1-10 in the mitochondrial matrix

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP1-10 mitochondrial matrix
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMT-IMD-V5-His

   Plasmid

   #63748

   Purpose

   Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   immune deficiency (imd Fly)
   

   Tags

   V5-6XHis

   Expression

   Insect

   Mutation

   *see comment below

   Promoter

   pMT

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-dCry-V5His

   Plasmid

   #136241

   Purpose

   Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   DmCry
   

   Tags

   His and V5

   Expression

   Mammalian

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LAMP1-GFP

   Plasmid

   #16290

   Depositor

   Ron Vale

   Article

   Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

   Insert

   LAMP1/RE72002
   

   Tags

   EGFP

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACUH-GFP11x7-mCherry-α-tubulin

   Plasmid

   #70218

   Purpose

   Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11x7-mCherry-α-tubulin
   

   Tags

   GFP11x7 and mCherry

   Expression

   Insect

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMesh1*

    Plasmid

    #175591

    Purpose

    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Mesh1 (Mesh1 Fly)
    

    Tags

    Fused with a flexible GS linker to cerulean

    Expression

    Bacterial

    Mutation

    Codon optimized for translation in E.coli. AGGAGG…

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KHBD00297

    Plasmid

    #34423

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG10798 (Myc Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+) Laccase2 MCS Exon Vector

    Plasmid

    #69893

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTattB-GFP-Msp300KASH

    Plasmid

    #170806

    Purpose

    Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

    Depositor

    Vikki Weake

    Article

    Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

    Insert

    GFP-Msp300KASH
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    5xUAS + hsp70 minimal promoter

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217515

    Purpose

    Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB1H2

    Plasmid

    #12613

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    dorsal RHR in pB1H2 (dl Fly)
    

    Tags

    flag

    Expression

    Bacterial

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00727

    Plasmid

    #39748

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9932 (CG9932 Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pJFRC12-10XUAS-IVS-myr::GFP

    Plasmid

    #26222

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PRExpress

    Plasmid

    #122486

    Purpose

    A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Inserts

    LacZ
    poly-PRE
    

    Expression

    Insect

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
    

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Available Since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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