3,636 results

1. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACUH-GFP11x7-mCherry-α-tubulin

   Plasmid

   #70218

   Purpose

   Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11x7-mCherry-α-tubulin
   

   Use

   Tags

   GFP11x7 and mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRExpress

   Plasmid

   #122486

   Purpose

   A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

   Depositor

   Renato Paro

   Article

   Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

   Inserts

   LacZ
   poly-PRE
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Andrew Allen, Ross Bathgate

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   DCA (same as CAG promoter)

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+) Laccase2 MCS Exon Vector

   Plasmid

   #69893

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   Laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC12-10XUAS-IVS-myr::GFP

   Plasmid

   #26222

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

   Plasmid

   #69905

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (HIPK3 Human, Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available Since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111545

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChrimson_tdtomato_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid

    #196972

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SCP1-pUC119

    Plasmid

    #21846

    Depositor

    James Kadonaga

    Article

    Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

    Insert

    SCP1 (super core promoter 1)-pUC119
    

    Use

    Puc derivative - a cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ovoD1 D1B2NR (18 kb)

    Plasmid

    #37280

    Depositor

    Norbert Perrimon

    Article

    Chou et al Development. 1993 Dec;119(4):1359-69.

    Insert

    ovoD1 genomic DNA fragment
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pUASP dsRed Rab7 WT

    Plasmid

    #44824

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. dmBACCS2-IRES-dOrai-IRES-mCherry

    Plasmid

    #72894

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Rab9

    Plasmid

    #31736

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab9 (Rab9 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pKC31

    Plasmid

    #226777

    Purpose

    Expresses Drosophila UNC-104(R562H) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R562H) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 562 to Histidine (bris mutation)

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQUAST

    Plasmid

    #24349

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    5xQUAS-Pmin promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge