Addgene

Showing: 1 - 17 of 17 results

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Availability

Academic Institutions and Nonprofits only
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Promoter

Availability

Academic Institutions and Nonprofits only
Mix.2-lux

Plasmid

#20893

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 promoter luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Use

Tags

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Promoter

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS

Plasmid

#20896

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

p53AS (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

5' natural EcoRV and SacI restriction sites …

Promoter

Availability

Academic Institutions and Nonprofits only
pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Promoter

Availability

Academic Institutions and Nonprofits only
pCS2-FLAG-hEcto/Tif1g

Plasmid

#20902

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal deletion of 37 aminoacids; Ectodermin …

Promoter

Availability

Academic Institutions and Nonprofits only
pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Promoter

Availability

Academic Institutions and Nonprofits only
8xGTIIC-luciferase

Plasmid

#34615

Purpose

YAP/TAZ-responsive synthetic promoter driving luciferase expression

Depositor

Stefano Piccolo

Article

Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

Insert

synthetic TEAD luciferase reporter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-EGFP

Plasmid

#84041

Purpose

3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-mCherry-cNLS

Plasmid

#138531

Purpose

Lentiviral inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-tdTomato-cNLS

Plasmid

#138532

Purpose

Lentiviral inducible expression of tdTomato with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

tdTomato

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-DsRed-cNLS

Plasmid

#138533

Purpose

Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

DsRed-Express2

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Availability

Academic Institutions and Nonprofits only

Enlarge