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pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

Plasmid

#101202

Purpose

Bacterial expression plasmid for SpCas9∆REC3 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant M1–N497,GGS,V713–D1368

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

M1–N497, GGS, V713–D1368

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

Plasmid

#101233

Purpose

Bacterial expression plasmid for SpCas9 Cluster 2 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant G582A/V583A/E584A/D585A/N588A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

G582A, V583A, E584A, D585A and N588A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pNHEJ-Cas9-recX

Plasmid

#158715

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Availability

Academic Institutions and Nonprofits only

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Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Availability

Academic Institutions and Nonprofits only

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pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

Plasmid

#101200

Purpose

Bacterial expression plasmid for SpCas9, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C and D273C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

Plasmid

#101201

Purpose

Bacterial expression plasmid for SpCas9, REC3 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/S701C/S960C

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, S701C and S960C

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

Plasmid

#101220

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A and Q926A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

Plasmid

#101234

Purpose

Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant T657A/G658A/W659A/R661A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

T657A, G658A, W659A, R661A and Q926A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

Plasmid

#101235

Purpose

Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant F491A/M495A/T496A/N497A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

F491A, M495A, T496A, N497A and Q926A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

Plasmid

#101236

Purpose

Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K918A/V922A/R925A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K918A, V922A, R925A and Q926A

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCas9-CAT

Plasmid

#80323

Purpose

Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Inserts

Cas9
Chloramphenicol acetyltransferase

Use

CRISPR

Tags

3X FLAG and NLS

Expression

Mutation

Promoter

TgTUB1

Availability

Academic Institutions and Nonprofits only

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pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

Plasmid

#110624

Purpose

A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

LP1B and hU6

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Availability

Academic Institutions and Nonprofits only

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pCas9-J23113

Plasmid

#119701

Purpose

Expression of Cas9 with a weak promoter

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AB65_pCAG.Cas9-D10A.rBGpA

Plasmid

#199256

Purpose

Expression construct encoding the SpCas9-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SpCas9.D10A nickase

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Availability

Academic Institutions and Nonprofits only

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pJWV102-wtcas9sp

Plasmid

#99612

Purpose

Plasmid containing wild-type cas9sp

Depositor

Jan-Willem Veening, Morten Kjos

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

cas9sp

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PczcD

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

Promoter

pG10-90

Availability

Academic Institutions and Nonprofits only

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