| pPS293 | GAL1 promoter (Saccharomyces cerevisiae) | Yeast Expression | |
Silver
|
pPS
(unpublished)
|
YEp352
GAL1 YBR020W
|
Add to Cart
|
| LTRi | | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| p404GAL1 | GAL1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p404ADH1
GAL1 YBR020W
|
Add to Cart
|
| p404GALL | GALL promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p404ADH1
GAL1 YBR020W
|
Add to Cart
|
| p404MET3 | MET3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p404ADH1
MET3
|
Add to Cart
|
| p404MET25 | MET25 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p404ADH1
MET17 YLR303W, MET15, MET25
|
Add to Cart
|
| p406MET25 | MET25 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p406ADH1
MET17 YLR303W, MET15, MET25
|
Add to Cart
|
| p406GALS | p406GALS (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p406ADH1
GAL1 YBR020W
|
Add to Cart
|
| p406GALL | GALL promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p406ADH1
GAL1 YBR020W
|
Add to Cart
|
| p404GALS | GALS promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| p406MET3 | MET3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
p406MET3-cyc1t
MET3
|
Add to Cart
|
| p406GAL1 | GAL1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae) | Bacterial Expression, Yeast Expression | |
Buchler
|
Buchler Lab plasmids
(unpublished)
|
pRS406
GAL1 YBR020W
|
Add to Cart
|
| pFIP | inversion switch | Bacterial Expression, Synthetic Biology | |
Keasling
| A tightly regulated inducible expression system utilizing the fim inversion recombination switch.
Biotechnol Bioeng. 2006 May 5. 94(1):1-4.
|
pBAD18
|
Add to Cart
|
| pPro24 | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro24
|
Add to Cart
|
| pPro18 | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro18
|
Add to Cart
|
| pPro18-cm | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro18-cm
|
Add to Cart
|
| pPro18-km | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro18-km
|
Add to Cart
|
| pPro30 | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro30
|
Add to Cart
|
| pPro33 | | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro33
|
Add to Cart
|
| pPro7(E)-gfp | GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| A Salmonella-based, propionate-inducible, expression system for Salmonella enterica.
Gene. 2006 Aug 1. 377():6-11.
|
n/a
|
Add to Cart
|
| pPro24(s)-gfp | GFPuv (Other) | Bacterial Expression, Synthetic Biology | |
Keasling
| Propionate-regulated high-yield protein production in Escherichia coli.
Biotechnol Bioeng. 2006 Apr 5. 93(5):912-8.
|
n/a
|
Add to Cart
|
| p70rg | E. coli optimized red flourescent protein (RFPec), GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.
Nat Biotechnol. 2006 Aug . 24(8):1027-32.
|
pBAD24
|
Add to Cart
|
| p70rg 1 | E. coli optimized red flourescent protein (RFPec), GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.
Nat Biotechnol. 2006 Aug . 24(8):1027-32.
|
pBAD24
|
Add to Cart
|
| p70rg 5 | E. coli optimized red flourescent protein (RFPec), GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.
Nat Biotechnol. 2006 Aug . 24(8):1027-32.
|
pBAD24
|
Add to Cart
|
| p70rg 11 | E. coli optimized red flourescent protein (RFPec), GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.
Nat Biotechnol. 2006 Aug . 24(8):1027-32.
|
pBAD24
|
Add to Cart
|
| p70rg 15 | E. coli optimized red flourescent protein (RFPec), GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes.
Nat Biotechnol. 2006 Aug . 24(8):1027-32.
|
pBAD24
|
Add to Cart
|
| pDL11 | alcA promoter, lacZ, argB, | Synthetic Biology ; Aspergillus nidulans expression | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pAST30
|
Add to Cart
|
| pDL11c | alcA promoter, lacZ, argB_1626, (Other) | Synthetic Biology ; Aspergillus nidulans expression | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pAST30
|
Add to Cart
|
| pDL12 | gpdA promoter, lacZ, argB, | Synthetic Biology ; Aspergillus nidulans expression | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pAST30
|
Add to Cart
|
| pDL12c | gpdA promoter, lacZ, argB_1626, (Other) | Synthetic Biology ; Aspergillus nidulans expression | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pAST30
|
Add to Cart
|
| pDL13 | alcA promoter, lacZ, trpC | Synthetic Biology ; A. nidulans | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pCN2
|
Add to Cart
|
| pDL14 | gpdA promoter, lacZ, trpC | Synthetic Biology ; A. nidulans | |
Keasling
| Marker and promoter effects on heterologous expression in Aspergillus nidulans.
Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
|
pCN2
|
Add to Cart
|
| pSPK1 | polyphosphate kinase | Bacterial Expression, Synthetic Biology | |
Keasling
| Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell.
Appl Environ Microbiol. 1997 May . 63(5):1689-95.
|
pMMB206
|
Add to Cart
|
| pSVD5 | exopolyphosphatase | Bacterial Expression, Synthetic Biology | |
Keasling
| Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell.
Appl Environ Microbiol. 1997 May . 63(5):1689-95.
|
pBAD18
|
Add to Cart
|
| pPro24-gfp | GFPuv | Bacterial Expression, Synthetic Biology | |
Keasling
| A propionate-inducible expression system for enteric bacteria.
Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
|
pPro24
|
Add to Cart
|
| pJAT13 | promoter CP13 | Bacterial Expression, Synthetic Biology | |
Keasling
| Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter.
Microbiology. 2001 Dec . 147(Pt 12):3241-7.
|
pJN105
|
Add to Cart
|
| pJAT13araE | araE | Bacterial Expression, Synthetic Biology | |
Keasling
| Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter.
Microbiology. 2001 Dec . 147(Pt 12):3241-7.
|
pJAT13
araE
|
Add to Cart
|
| pAK02 | arabinose transporter araE under control of Ptac | Bacterial Expression, Synthetic Biology | |
Keasling
| Regulatable arabinose-inducible gene expression system with consistent control in all cells of a culture.
J Bacteriol. 2000 Dec . 182(24):7029-34.
|
pMMB207
araE
|
Add to Cart
|
| TLD_026 | | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| TLD_009 | EGFP | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| TLD_010 | EGFP | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| TLD_027 | | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| TLD_062 | | Mammalian Expression, Synthetic Biology | |
Collins
| A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells.
Cell. 2007 Jul 27. 130(2):363-72.
|
LTRi
|
Add to Cart
|
| pEF 1-PNH-4 | PNH promoter | Synthetic Biology ; plant Gateway Donor vector | |
Richart
|
FQG plasmids
(unpublished)
|
pDONRP4-P1R
|
Add to Cart
|
| pMLLCK-K112503 | C terminal myc tag | Synthetic Biology ; 2ab assembly vector | |
Anderson
|
J.C. Anderson Lab Plasmids
(unpublished)
|
pMLL-CK
|
Add to Cart
|
| pTRKH3-slpGFP | surface (S)-layer protein promoter region fused with modified green fluorescent protein 5 | Bacterial Expression | |
Lizier
| Comparison of expression vectors in Lactobacillus reuteri strains.
FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
|
pTRKH3
|
Add to Cart
|
| pTRKH3-ermGFP | erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5 | Bacterial Expression | |
Lizier
| Comparison of expression vectors in Lactobacillus reuteri strains.
FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
|
pTRKH3
|
Add to Cart
|
| pBS-ldhGFP | lactate dehydrogenase promoter region fused with modified green fluorescent protein 5 | Bacterial Expression | |
Lizier
| Comparison of expression vectors in Lactobacillus reuteri strains.
FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
|
pBluescript II KS(+)
|
Add to Cart
|
| pBS-slpGFP | surface (S)-layer protein promoter region fused with modified green fluorescent protein 5 | Bacterial Expression | |
Lizier
| Comparison of expression vectors in Lactobacillus reuteri strains.
FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
|
pBluescript II KS(+)
|
Add to Cart
|
| pBS-ermGFP | erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5 | Bacterial Expression | |
Lizier
| Comparison of expression vectors in Lactobacillus reuteri strains.
FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
|
pBluescript II KS(+)
|
Add to Cart
|
| SS13 lac-inducible GFP reporter circuit | GFP | Bacterial Expression, Synthetic Biology | |
Sauro
| Designing and engineering
evolutionary robust genetic circuits
J Biol Eng. 2010 Nov 1;4:12
|
pSB1A2
|
Add to Cart
|
| pHL600 | CsrB, CsrA | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
csrB b4408, ECK2787, JWR0062
csrA b2696, ECK2691, zfiA
|
Add to Cart
|
| pHL601 | CsrC, CsrA | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
csrC b4457, ECK3858, IS198, JWR0214, psrA21, ryiB, sraK, tpk2
csrA b2696, ECK2691, zfiA
|
Add to Cart
|
| pHL1355 | GFP, TetR, CsrA | Bacterial Expression, Synthetic Biology | pConNoHindM12, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
Tag
/ Fusion Protein- glgC leader region (N terminal on backbone)
|
Add to Cart
|
| pHL1530 | GFP, TetR, CsrA | Bacterial Expression, Synthetic Biology | pLlacO-1, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
Tag
/ Fusion Protein- glgC leader region (N terminal on backbone)
|
Add to Cart
|
| pHL1559 | CsrB | Bacterial Expression, Synthetic Biology | pLtetO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
csrB b4408, ECK2787, JWR0062
|
Add to Cart
|
| pHL1561 | CsrA | Bacterial Expression, Synthetic Biology | pLtetO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
csrA b2696, ECK2691, zfiA
|
Add to Cart
|
| pHL1668 | GFP, TetR, CsrA | Bacterial Expression, Synthetic Biology | pConM12NoHind, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
csrA b2696, ECK2691, zfiA
|
Add to Cart
|
| pHL1670 | GFP, TetR, CsrA | Bacterial Expression, Synthetic Biology | pConM12NoHind, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
Tags
/ Fusion Proteins- ASV degradation tag (C terminal on backbone)
- glgC leader region (N terminal on backbone)
csrA b2696, ECK2691, zfiA
|
Add to Cart
|
| pHL661 | GFP, mCherry | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
|
Add to Cart
|
| pHL662 | mCherry, GFP | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with p15a origin
|
Add to Cart
|
| pHL1318 | TetR, CsrA | Bacterial Expression, Synthetic Biology | pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
|
Add to Cart
|
| pHL1324 | GFP, TetR, CsrA | Bacterial Expression, Synthetic Biology | pCon, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
pZ with ColE origin
|
Add to Cart
|
| pHL 233 | ptet_ompC_gfp, pLac_st7 TetR_mCherry | Synthetic Biology ; E. coli | ptet, pLac |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
ColE (from pZ system) + AmpR
|
Add to Cart
|
| pHL 279 | ptet_ompC_gfp, pLac_st3 TetR_mCherry | Synthetic Biology ; E. coli | ptet, pLac |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
ColE (from pZ system) + AmpR
Tags
/ Fusion Proteins- gfp (C terminal on insert)
- mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 363 | pLar_rpoS_gfp, pLtet_dsrA_pLac_araC | Synthetic Biology ; E. coli | pLar, Ptet and pLac |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
ColE (from pZ system) + AmpR
Tags
/ Fusion Proteins- gfp (C terminal on insert)
- none
|
Add to Cart
|
| pHL 1569 | ptet_ompC_gfpAaV, pLac_micC_mCherry | Synthetic Biology ; E. coli | ptet, pLac |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
ColE (from pZ system) + AmpR
Tags
/ Fusion Proteins- gfp (C terminal on insert)
- mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 1571 | ptet_ompC_gfpAaV, pLac_st3 TetR_mCherry | Synthetic Biology ; E. coli | ptet, pLac |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
ColE (from pZ system) + AmpR
Tags
/ Fusion Proteins- gfp (C terminal on insert)
- mCherry (C terminal on insert)
|
Add to Cart
|
| pSB4A5 JQ929581 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; BioBrick cloning vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
pSB4A5
|
Add to Cart
|
| pSB4A5 JQ929582 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; BioBrick cloning vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
pSB4A5
|
Add to Cart
|
| Phi80 JQ929581 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; Integration vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
Phi80
|
Add to Cart
|
| Phi80 JQ929582 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; Integration vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
Phi80
|
Add to Cart
|
| Strain Phi80 JQ929581 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; Integration vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
Phi80
|
Add to Cart
|
| Strain Phi80 JQ929582 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; Integration vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
Phi80
|
Add to Cart
|
| Strain HK022 JQ929581 | mKate2 (Synthetic), Superfolder GFP (Synthetic) | Synthetic Biology ; Integration vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
Phi80
|
Add to Cart
|
| pBad-Xis/Int Reset Flipper | Bxb1-Excisionase, Bxb1 Integrase | Synthetic Biology ; BioBrick cloning vector | pBAD |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
J64100
|
Add to Cart
|
| RAD Module G8-C1 | SET Integrase, Bxb1 Excisonase, RESET Integrase | Synthetic Biology ; BioBrick cloning vector | |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
J64100
|
Add to Cart
|
| pBad-Int Set Flipper | Bxb1 Integrase | Synthetic Biology ; BioBrick cloning vector | pBAD/AraC |
Endy
| Rewritable digital data storage in live cells via engineered control of recombination directionality.
Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.
|
pSB3K1
|
Add to Cart
|
| pSTC0 | | Bacterial Expression, Synthetic Biology | |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSB1AK3
|
Add to Cart
|
| pSTC1 | | Bacterial Expression, Synthetic Biology | |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSB4K5
|
Add to Cart
|
| pSTC0r1 | pLlacO-1+BBa_B0034+GFP | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ11 | RAJ11 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ11m | RAJ11 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ12 | RAJ12 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ12m | RAJ12 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ12f | RAJ12 system (no terminator for the transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ21 | RAJ21 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ21m | RAJ21 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ22 | RAJ22 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ22m | RAJ22 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ23 | RAJ23 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ23m | RAJ23 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC0
|
Add to Cart
|
| pRAJ31 | RAJ31 system | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ31m | RAJ31 system (no transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ31f | RAJ31 system (no terminator for the transRNA) | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pSTC1
|
Add to Cart
|
| pRAJ11-12 | transRAJ11+cisRAJ12 | Bacterial Expression, Synthetic Biology | pLlacO-1+pLtetO-1 |
Jaramillo
| De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep 4.
|
pRAJ12
|
Add to Cart
|
| SW5 | Bistable switch using dynamic degradation | Bacterial Expression, Synthetic Biology | |
Maharbiz
| A genetic bistable switch utilizing nonlinear protein degradation.
J Biol Eng. 2012 Jul 9;6(1):9.
|
psc101
|
Add to Cart
|
| PLlacO-1-CImf | Lac inducible lambda repressor | Bacterial Expression, Synthetic Biology | |
Maharbiz
| A genetic bistable switch utilizing nonlinear protein degradation.
J Biol Eng. 2012 Jul 9;6(1):9.
|
ColE1
|
Add to Cart
|
| PRM-GFP | CI inducible sfGFP | Bacterial Expression, Synthetic Biology | pRM |
Maharbiz
| A genetic bistable switch utilizing nonlinear protein degradation.
J Biol Eng. 2012 Jul 9;6(1):9.
|
p15A
|
Add to Cart
|
| pCYC2 | | Bacterial Expression, Yeast Expression, Synthetic Biology | CYC1 |
Alper
| Re-engineering multicloning sites for function and convenience.
Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
|
p416-CYC
|
Add to Cart
|
| pTEF2 | | Bacterial Expression, Yeast Expression, Synthetic Biology | TEF |
Alper
| Re-engineering multicloning sites for function and convenience.
Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
|
p416-TEF
|
Add to Cart
|
| pGPD2 | | Bacterial Expression, Yeast Expression, Synthetic Biology | GPD |
Alper
| Re-engineering multicloning sites for function and convenience.
Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
|
p416-GPD
|
Add to Cart
|
| pUC-UAS1B1-Leum | UAS1B1-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B1-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B2-Leum | UAS1B2-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B2-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
Tag
/ Fusion Protein |
Add to Cart
|
| pUC-UAS1B3-Leum | UAS1B3-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B3-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
Tag
/ Fusion Protein |
Add to Cart
|
| pUC-UAS1B4-Leum | UAS1B4-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B4-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B5-Leum | UAS1B5-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B5-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B6-Leum | UAS1B6-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B6-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B7-Leum | UAS1B7-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B7-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-Leum | UAS1B8-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B8-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B9-Leum | UAS1B9-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B9-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B10-Leum | UAS1B10-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B10-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B11-Leum | UAS1B11-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B11-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B12-Leum | UAS1B12-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B12-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B13-Leum | UAS1B13-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B13-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B14-Leum | UAS1B14-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B14-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B15-Leum | UAS1B15-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B15-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B20-Leum | UAS1B20-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B20-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B28-Leum | UAS1B28-Leum promoter | Bacterial Expression, Synthetic Biology | UAS1B28-Leum |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-Leum | Leum promoter | Bacterial Expression, Synthetic Biology | Leum promoter |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B16-TEF(1004) | UAS1B16-TEF(1004) promoter | Bacterial Expression, Synthetic Biology | UAS1B16-TEF(1004) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(1004) | UAS1B8-TEF(1004) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(1004) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(804) | UAS1B8-TEF(804) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(804) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B16-TEF(504) | UAS1B16-TEF(504) promoter | Bacterial Expression, Synthetic Biology | UAS1B16-TEF(504) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(504) | UAS1B8-TEF(504) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(504) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF | UAS1B8-TEF promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B16-TEF(272) | UAS1B16-TEF(272) promoter | Bacterial Expression, Synthetic Biology | UAS1B16-TEF(272) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(272) | UAS1B8-TEF(272) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(272) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B16-TEF(203) | UAS1B16-TEF(203) promoter | Bacterial Expression, Synthetic Biology | UAS1B16-TEF(203) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(203) | UAS1B8-TEF(203) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(203) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B16-TEF(136) | UAS1B16-TEF(136) promoter | Bacterial Expression, Synthetic Biology | UAS1B16-TEF(136) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| pUC-UAS1B8-TEF(136) | UAS1B8-TEF(136) promoter | Bacterial Expression, Synthetic Biology | UAS1B8-TEF(136) |
Alper
| Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach.
Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
|
pUC19
|
Add to Cart
|
| AND-gate-V2.0 | AND-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| OR gate | OR-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| NOR gate-V2.0 | NOR-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| XOR gate-V2.0 | XOR-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| XNOR-gate-V2.0 | XNOR-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| XNOR-gate-V2.0-ASV | XNOR-gate | Bacterial Expression, Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
pSB4A5
|
Add to Cart
|
| Dual-recombinase-controller | pTEt-Bxb1, pBAD-TP901 | Synthetic Biology | |
Endy
| Amplifying genetic logic gates.
Science. 2013 May 3;340(6132):599-603. doi: 10.1126/science.1232758. Epub 2013 Mar 28.
|
J64100
|
Add to Cart
|
| pDN-G1Gh | PGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-D12 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS403
GAL1 YBR020W
|
Add to Cart
|
| pDN-G1TGt | PGal1-D12 promoter (Saccharomyces cerevisiae), tetR::yEGFP (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-D12 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| pDN-G1Tt | PGal1-D12 promoter (Saccharomyces cerevisiae), tetR (Synthetic) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-D12 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| pDN-T1TCbt | PGal1-T123 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-T123 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| pDN-G1TCbt | PGal1-D12 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-D12 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| pDN-G1GZmbh | PGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-D12 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS403
GAL1 YBR020W
|
Add to Cart
|
| pDN-T1GZmbh | PGal1-T123 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1-T123 promoter |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS403
GAL1 YBR020W
|
Add to Cart
|
| pDN-NG1Tt | PGal1 (wt) promoter (Saccharomyces cerevisiae), tetR (Synthetic) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PGal1 (wt) |
Balazsi
| Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
|
pRS404
GAL1 YBR020W
|
Add to Cart
|
| pDN-T2dGZmxh | PTETREG promoter (Synthetic), yEGFP::ZeoR (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PTETREG |
Balazsi
| Mapping the environmental fitness landscape of a synthetic gene circuit.
PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
|
pRS403
|
Add to Cart
|
| pDN-T2dMFot | PTETREG promoter (Synthetic), rtetR-M2::FFF (Synthetic) | Yeast Expression, Synthetic Biology ; Expression regulator/reporter | PTETREG |
Balazsi
| Mapping the environmental fitness landscape of a synthetic gene circuit.
PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
|
pRS404
|
Add to Cart
|
| pDN-D2irTNG4kwh | pCMV-D2i promoter (Synthetic), htetR::NLS::EGFP (Homo sapiens) | Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system | pCMV-D2i |
Balazsi
| Transferring a synthetic gene circuit from yeast to mammalian cells.
Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
|
pcDNA4/TO
|
Add to Cart
|
| pDN-D2irG4kwh | pCMV-D2i promoter (Synthetic), EGFP (Homo sapiens) | Mammalian Expression, Synthetic Biology ; Expression regulator/reporter | pCMV-D2i |
Balazsi
| Transferring a synthetic gene circuit from yeast to mammalian cells.
Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
|
pcDNA4/TO
Tags
/ Fusion Proteins- Rabbit β-globin intron II (N terminal on insert)
- WPRE (C terminal on insert)
|
Add to Cart
|
| pDN-D2irTN6kwh | pCMV-D2i promoter (Synthetic), htetR::NLS (Homo sapiens) | Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system | pCMV-D2i |
Balazsi
| Transferring a synthetic gene circuit from yeast to mammalian cells.
Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
|
pcDNA6/TR
Tags
/ Fusion Proteins- Rabbit β-globin intron II (N terminal on insert)
- WPRE (C terminal on insert)
|
Add to Cart
|
| pDN-D2irC6kwh | pCMV-D2i promoter (Synthetic), mCherry (Homo sapiens) | Mammalian Expression, Synthetic Biology ; Expression regulator/reporter | pCMV-D2i |
Balazsi
| Transferring a synthetic gene circuit from yeast to mammalian cells.
Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
|
pcDNA6/TR
Tags
/ Fusion Proteins- Rabbit β-globin intron II (N terminal on insert)
- WPRE (C terminal on insert)
|
Add to Cart
|
| PLlacO-1-mfLon | Lon | Bacterial Expression, Synthetic Biology | PLlacO-1 |
Maharbiz
| A genetic bistable switch utilizing nonlinear protein degradation.
J Biol Eng. 2012 Jul 9;6(1):9.
|
pBbC6c-RFP
|
Add to Cart
|
| pBEST-PLtetO-1-UTR1-TetR-deGFP-T500 | tetR-deGFP (Synthetic) | Bacterial Expression, Synthetic Biology | PLtetO-1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-PLtetO-1-UTR1-LacI-YbaQ-T500 | Lac repressor | Bacterial Expression, Synthetic Biology | PLtetO-1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-PLlacO-1-UTR1-deCFP-T500 | deCFP (Synthetic) | Bacterial Expression, Synthetic Biology | PLlacO-1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500 | Tetracycline repressor | Bacterial Expression, Synthetic Biology | PLlacO-1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500 | sigma factor 28 | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr (bacteriophage Lambda with one mutation) |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-pTar-UTR1-TetR-T500 | Tetracycline repressor | Bacterial Expression, Synthetic Biology | pTar |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-p15a-PLtetO-1-UTR1-LacI-T500 | Lac repressor | Bacterial Expression, Synthetic Biology | PLtetO-1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-OR2-OR1-Pr-UTR1-araC-T500 | araC | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr (bacteriophage Lambda with one mutation) |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-p15a-pBAD-UTR1-TetR-T500 | Tetracycline repressor | Bacterial Expression, Synthetic Biology | pBAD |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500 | deGFP (Synthetic) | Bacterial Expression, Synthetic Biology | pBAD-tetO1 |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA | deGFP, Tetracycline repressor, araC | Bacterial Expression, Synthetic Biology | pBAD-tetO1, pBAD, OR2-OR1-Pr (bacteriophage Lambda with one mutation) |
Murray
|
Murray Lab pBEST plasmids
(unpublished)
|
pBEST-Luc
|
Add to Cart
|
| pGR-BBa_B0010 | BBa_B0010 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-L3S2P21 | L3S2P21 Terminator (Synthetic) | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-L3S2P00 | L3S2P00 Terminator (Synthetic) | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-ECK120015170 | ECK120015170 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-L3S2P36 | L3S2P36 Terminator (Synthetic) | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-ECK125108723 | ECK125108723 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-ECK120010783 | ECK120010783 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-ECK120010800 | ECK120010800 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pGR-ECK120033127 | ECK120033127 Terminator | Bacterial Expression, Synthetic Biology | PBAD |
Voigt
| Characterization of 582 natural and synthetic terminators and quantification of their design constraints.
Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
|
pGR
|
Add to Cart
|
| pCOCK CMVwt | CMV wt promoter (Other) | Mammalian Expression, Synthetic Biology | CMV |
Wang
| Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.
Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
|
Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.
pEF5-FRT-V5-DEST
|
Add to Cart
|
| pCOCK CM-C18 | CMV mutant promoter (Other) | Mammalian Expression, Synthetic Biology | |
Wang
| Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.
Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
|
Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
pEF5-FRT-V5-DEST
|
Add to Cart
|
| pCOCK CM-C02 | CMV mutant promoter (Other) | Mammalian Expression, Synthetic Biology | |
Wang
| Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.
Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
|
Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).
pEF5-FRT-V5-DEST
|
Add to Cart
|
| pCOCK CM-R03 | CMV mutant promoter (Other) | Mammalian Expression, Synthetic Biology | |
Wang
| Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.
Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
|
Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
pEF5-FRT-V5-DEST
|
Add to Cart
|
| pCOCK CM-R05 | CMV mutant promoter (Other) | Mammalian Expression, Synthetic Biology | |
Wang
| Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.
Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
|
Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
pEF5-FRT-V5-DEST
|
Add to Cart
|
| pFAB3905 | promoter 14 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3906 | promoter 14 and BCD2 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3909 | promoter 14 and BCD5 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3910 | promoter 14 and BCD6 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD6 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3911 | promoter 14 and BCD7 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD7 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3912 | promoter 14 and BCD8 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3913 | promoter 14 and BCD9 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3914 | promoter 14 and BCD10 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3915 | promoter 14 and BCD11 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3916 | promoter 14 and BCD12 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD12 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3989 | promoter 14 and BCD13 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3991 | promoter 14 and BCD15 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3992 | promoter 14 and BCD16 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3993 | promoter 14 and BCD17 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3994 | promoter 14 and BCD18 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3995 | promoter 14 and BCD19 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3996 | promoter 14 and BCD20 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3997 | promoter 14 and BCD21 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3998 | promoter 14 and BCD22 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3999 | promoter 14 and BCD23 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB4000 | promoter 14 and BCD24 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3665 | promoter 1 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3677 | promoter 2 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3689 | promoter 3 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3701 | promoter 4 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3713 | promoter 5 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 5 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3725 | promoter 6 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3737 | promoter 7 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3833 | promoter 8 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3845 | promoter 9 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3857 | promoter 10 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3869 | promoter 11 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3881 | promoter 12 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB3893 | promoter 13 and BCD1 (Synthetic) | Bacterial Expression, Synthetic Biology | see insert |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.
pFAB1781
|
Add to Cart
|
| pFAB801 | trp_att_L126 terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO1 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiency
pFAB763
|
Add to Cart
|
| pFAB803 | T7 early terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO2 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring T7 early termination efficiency
pFAB764
|
Add to Cart
|
| pFAB804 | T3 early terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO3 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring T3 early termination efficiency
pFAB765
|
Add to Cart
|
| pFAB806 | lambda tR2 terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO4 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency
pFAB766
|
Add to Cart
|
| pFAB805 | tonB terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO5 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring tonB termination efficiency
pFAB767
|
Add to Cart
|
| pFAB816 | ilvGEDA (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO6 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency
pFAB768
|
Add to Cart
|
| pFAB807 | RNAI terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO7 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring RNAI termination efficiency
pFAB769
|
Add to Cart
|
| pFAB808 | rrnB T1 terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO8 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency
pFAB770
|
Add to Cart
|
| pFAB815 | rnpB T1 terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO9 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency
pFAB771
|
Add to Cart
|
| pFAB820 | tetAC terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO10 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring tetAC termination efficiency
pFAB772
|
Add to Cart
|
| pFAB822 | BBa_B1006 terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO11 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency
pFAB773
|
Add to Cart
|
| pFAB1907 | BBa_B1006 U10 (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO12 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency
pFAB774
|
Add to Cart
|
| pFAB1902 | M13_central_T_linker_rrnD_T1 double terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO13 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency
pFAB775
|
Add to Cart
|
| pFAB1910 | rpoC terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO14 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring rpoC termination efficiency
pFAB776
|
Add to Cart
|
| pFAB1935 | his_T_min_linker_crp_T_min double terminator (Synthetic) | Bacterial Expression, Synthetic Biology | pLTetO15 |
Endy
| Precise and reliable gene expression via standard transcription and translation initiation elements.
Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10.
|
BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency
pFAB777
|
Add to Cart
|
| pJS167 | araC gene with ssrA tag, monomeric yeast-enhanced green fluorescent protein | Bacterial Expression, Synthetic Biology | Para/lac |
Hasty
| A fast, robust and tunable synthetic gene oscillator.
Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29.
|
Positive feedback plasmid from oscillator
pZ vector
|
Add to Cart
|
| pJS169 | lacI with ssrA tag | Bacterial Expression, Synthetic Biology | Para/lac |
Hasty
| A fast, robust and tunable synthetic gene oscillator.
Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29.
|
Negative feedback plasmid from oscillator
pZ vector
|
Add to Cart
|
| pTPGI_dCas9_VP64 | dCas9_VP64 (codon-optimized for expression in S. cerevisiae) (Saccharomyces cerevisiae) | Yeast Expression, CRISPR, Synthetic Biology | pTPGI (galactose+aTc inducible) |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
encodes yeast-optimized dCas9_VP64 synthetic transcription factor
pTPGI (pRS304)
|
Add to Cart
|
| pRPR1_gRNA_handle_RPR1t | | Yeast Expression, CRISPR, Synthetic Biology | pRPR1 |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
gRNA expression empty vector (yeast)
pRS425
|
Add to Cart
|
| pCMV_dCas9_VP64 | dCas9_VP64 (human-codon-optimized) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
encodes human-optimized dCas9_VP64 synthetic transcription factor
phi-Yellow-Dest
|
Add to Cart
|
| pU6_gRNA_handle_U6t | | Mammalian Expression, CRISPR, Synthetic Biology | |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
gRNA expression empty vector (mammalian cells)
phi-Yellow-Dest
|
Add to Cart
|
| pRF-AmtR | AmtR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-AmeR | AmeR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-BetI | BetI repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-BM3R1 | BM3R1 repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-ButR | ButR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-HapR | HapR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-HlyIIR | HlyIIR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-IcaR | IcaR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-LitR | LitR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-LmrA | LmrA repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-McbR | McbR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-Orf2 | Orf2 repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-PhlF | PhlF repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-PsrA | PsrA repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-QacR | QacR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-ScbR | ScbR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-SmcR | SmcR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-SrpR | SrpR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-TarA | TarA repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pRF-TetR | TetR repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pLux-LmrA | LmrA repressor (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pCI-YFP
|
Add to Cart
|
| pNAND-PhlF | PhlF repressor, pPhlF-pLmrA reporter (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pEXT20
|
Add to Cart
|
| pAND | AND gate (Other) | Bacterial Expression, Synthetic Biology | |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
Expression of AND gate in bacteria
pEXT20
|
Add to Cart
|
| pBI101-pGC1(D1)-GUS | Promoter of GC1(D1) (Arabidopsis thaliana) | Plant expression | GC1 (At1g22690), 1141 bp upstream of the transcriptional start |
Schroeder
| Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool.
Plant Methods. 2008 Feb 19;4:6. doi: 10.1186/1746-4811-4-6.
|
Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant
pBI101
|
Add to Cart
|
| pVRa01_3473 | ECF01_3473 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa02_915 | ECF02_915 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa02_2817 | ECF02_2817 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa03_1198 | ECF03_1198 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa03_1244 | ECF03_1244 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa04_1609 | ECF04_1609 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa04_1617 | ECF04_1617 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa07_980 | ECF07_980 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa07_1134 | ECF07_1134 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa11_987 | ECF11_987 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa11_3726 | ECF11_3726 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa12_807 | ECF12_807 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa12_808 | ECF12_808 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa14_1324 | ECF14_1324 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa14_3200 | ECF14_3200 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa15_436 | ECF15_436 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa15_524 | ECF15_524 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa16_973 | ECF16_973 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa16_3622 | ECF16_3622 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa17_1458 | ECF17_1458 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa17_1691 | ECF17_1691 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa20_992 | ECF20_992 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa20_2913 | ECF20_2913 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa22_1147 | ECF22_1147 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa22_4450 | ECF22_4450 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa25_1643 | ECF25_1643 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa25_1645 | ECF25_1645 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa26_837 | ECF26_837 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa26_4464 | ECF26_4464 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa27_1331 | ECF27_1331 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa27_4265 | ECF27_4265 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa28_1088 | ECF28_1088 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa29_371 | ECF29_371 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa30_35 | ECF30_35 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa30_83 | ECF30_83 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa31_34 | ECF31_34 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa31_2963 | ECF31_2963 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa32_1122 | ECF32_1122 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa32_3724 | ECF32_3724 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa33_375 | ECF33_375 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa33_423 | ECF33_423 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa34_1384 | ECF34_1384 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa34_3302 | ECF34_3302 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa35_3582 | ECF35_3582 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa37_2513 | ECF37_2513 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa37_3390 | ECF37_3390 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa38_1322 | ECF38_1322 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa38_1442 | ECF38_1442 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa39_2973 | ECF39_2973 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa40_3198 | ECF40_3198 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa41_491 | ECF41_491 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRa42_4454 | ECF42_4454 (Other) | Bacterial Expression, Synthetic Biology | pT7-lacO |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRa
Tag
/ Fusion Protein- His6-PreScission (N terminal on backbone)
|
Add to Cart
|
| pVRb01_syn20 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf01_syn20 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb02_2817 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf02_2817 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb03_up1198 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf03_up1198 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb11_3726 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf11_3726 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb12_up807 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf12_up807 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb15_up436 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf15_up436 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb16_3622 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf16_3622 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb17_up1691 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf17_up1691 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb18_up1700 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf18_up1700 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb19_up1315 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf19_up1315 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb20_992 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf20_992 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb21_up4014 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf21_up4014 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb22_up1147 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf22_up1147 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb25_up4311 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf25_up4311 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb26_up601 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf26_up601 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb29_up371 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf29_up371 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb30_2079 | sfgfp (Synthetic) | Bacterial Expression, Synthetic Biology | Pecf30_2079 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb31_34 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf31_34 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb32_1122 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf32_1122 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb33_375 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf33_375 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb38_up1322 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf38_up1322 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb39_up1413 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf39_up1413 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb41_up1141 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf41_up1141 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRb42_up4062 | sfgfp (Other) | Bacterial Expression, Synthetic Biology | Pecf42_up4062 |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRb
|
Add to Cart
|
| pVRc01_3473 | AS01_3473 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc02_915 | AS02_915 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc03_1198 | AS03_1198 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc03_1244 | AS03_1244 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc04_1609 | AS04_1609 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc11_987 | AS11_987 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc14_1324 | AS14_1324 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc15_436 | AS15_436 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc15_524 | AS15_524 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc16_973 | AS16_973 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc16_3622 | AS16_3622 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc20_992 | AS20_992 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc22_1147 | AS22_1147 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc22_4450 | AS22_4450 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc25_1643_cl43 | AS25_1643_cl43 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc25_1645 | AS25_1645 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc27_1331 | AS27_1331 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc27_4265 | AS27_4265 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc30_35 | AS30_35 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc31_34 | AS31_34 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc33_375 | AS33_375 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc33_423 | AS33_423 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc35_3582 | AS35_3582 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc37_2513 | AS37_2513 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pVRc38_1322 | AS38_1322 (Other) | Bacterial Expression, Synthetic Biology | pLux |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
pVRc
|
Add to Cart
|
| pN565 | T7 RNAP* (Other) | Bacterial Expression, Synthetic Biology | pTac-symmetric |
Voigt
| Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters.
Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
|
expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control
pIncW
|
Add to Cart
|
| pBL4 | CcaS (Other), CcaR (Other) | Bacterial Expression, Synthetic Biology | |
Tabor
| Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
|
Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoter
pProTet.E333
|
Add to Cart
|
| pBL6 | mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | |
Tabor
| Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
|
Expresses mCherry under the PLtetO-1 promoter
pSB4A3
|
Add to Cart
|
| pEO100c | sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Tabor
| Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
|
Expresses sfGFP under the PompC1157 promoter
pSB4A3
|
Add to Cart
|
| pJT119b | CcaS (Other), CcaR (Other), sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Tabor
| Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
|
Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter
pProTet.E333
|
Add to Cart
|
| pCph8 | Cph8 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Tabor
| Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals.
Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
|
Expresses Cph8 under a modified PLtetO-1 promoter
pProTet.E333
|
Add to Cart
|
| pBBRBB-Ppuf1-1200-DsRed | DsRed | Synthetic Biology | Ppuf1-1200 |
Schmidt-dannert
| BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides.
Appl Microbiol Biotechnol. 2014 Feb 9.
|
Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroides
pBBRBB
|
Add to Cart
|
| pBBRBB-Ppuf843-1200-DsRed | DsRed | Synthetic Biology | Ppuf843-1200 |
Schmidt-dannert
| BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides.
Appl Microbiol Biotechnol. 2014 Feb 9.
|
Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides
pBBRBB
|
Add to Cart
|
| pBBRBB-Ppuf843-1200-PR | Proteorhodopsin (Other) | Bacterial Expression, Synthetic Biology | Ppuf843-1200 |
Schmidt-dannert
| BioBrick compatible vector system for protein expression in Rhodobacter sphaeroides.
Appl Microbiol Biotechnol. 2014 Feb 9.
|
Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides
pBBRBB
|
Add to Cart
|
| pET21a-AcrR | AcrR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-AmeR | AmeR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-AmrR | AmrR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-AmtR | AmtR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BarA | BarA (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BarB | BarB (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BetI | BetI (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BM1P1 | BM1P1 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BM3R1 | BM3R1 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-BpeR | BpeR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-CalR1 | CalR1 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-CampR | CampR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-CasR | CasR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-CprB | CprB (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-CymR | CymR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-Cyp106 | Cyp106 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-DhaR | DhaR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-Ef0113 | Ef0113 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-EthR | EthR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-HapR | HapR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-HemR | HemR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-HlyIIR | HlyIIR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-IcaR-e | IcaR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-IfeR | IfeR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-JadR2 | JadR2 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-KstR | KstR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-LitR | LitR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-LuxT | LuxT (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-McbR | McbR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-MtrR | MtrR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-NonG | NonG (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-Orf2 | Orf2 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-orfL6 | orfL6 (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-PaaR | PaaR (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-PqrA | PqrA (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pET21a-TylP | TylP (Other) | Bacterial Expression, Synthetic Biology | T7 |
Voigt
| Genomic mining of prokaryotic repressors for orthogonal logic gates.
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
|
pET21a
Tag
/ Fusion Protein- 6x-his (N terminal on backbone)
|
Add to Cart
|
| pExp_CAG:EBFP2 | EBFP2 (Synthetic) | Mammalian Expression, Synthetic Biology | CAG |
Weiss
| Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition.
ACS Synth Biol. 2014 Nov 17.
|
Constitutively expresses blue fluorescent protein, EBFP2
pZDONOR 5-6
|
Add to Cart
|
| pExp_CAG:EYFP | EYFP | Mammalian Expression, Synthetic Biology | CAG |
Weiss
| Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition.
ACS Synth Biol. 2014 Nov 17.
|
Constitutively expresses yellow fluorescent protein , EYFP
pZDONOR 5-6
|
Add to Cart
|
| pExp_TRE:EBFP2 | EBFP2 | Mammalian Expression, Synthetic Biology | TRE |
Weiss
| Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition.
ACS Synth Biol. 2014 Nov 17.
|
Inducible expression of EBFP2 with rtTA for input fluorescence
pZDONOR 5-6
|
Add to Cart
|
| pExp_TRE:TAL14 | TAL14 | Mammalian Expression, Synthetic Biology | TRE |
Weiss
| Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition.
ACS Synth Biol. 2014 Nov 17.
|
Inducible expression of TAL14 as input repressor
pZDONOR 5-6
|
Add to Cart
|
| pExp_TRE:TAL21 | TAL21 | Mammalian Expression, Synthetic Biology | TRE |
Weiss
| Accurate Predictions of Genetic Circuit Behavior from Part Characterization and Modular Composition.
ACS Synth Biol. 2014 Nov 17.
|
Inducible expression of TAL21 as input repressor
pZDONOR 5-6
|
Add to Cart
|
| pHL 100 | OxyS sRNA (Other), fhlA (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1, pLtetO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
unknown
Tag
/ Fusion Protein- RBS-mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 762 | MicC sRNA (Other), sodB (Other), ompC (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLtetO-1, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
unknown
|
Add to Cart
|
| pHL 802 | OxyS sRNA (Other), ompC (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
unknown
Tag
/ Fusion Protein- RBS-mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 990 | DsrA sRNA (Other), RhyB sRNA (competitor) (Other), rpoS (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp
unknown
|
Add to Cart
|
| pHL 1011 | RhyB sRNA (Other), RBS (st7) (Synthetic) | Bacterial Expression, Synthetic Biology | pLlac0-1, pLtetO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
unknown
Tag
/ Fusion Protein- RBS-mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 1019 | RhyB sRNA (Other), MicC sRNA (Competitor) (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1020 | RhyB sRNA (Other), DsrA sRNA (competitor) (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1022 | RhyB sRNA (Other), OxyS sRNA (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1032 | RhyB sRNA (Other), ompC (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1033 | RhyB sRNA (Other), rpoS (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1035 | RhyB sRNA (Other), fhlA (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1039 | DsrA sRNA (Other), OxyS sRNA (competitor) (Other), rpoS (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp
unknown
|
Add to Cart
|
| pHL 1057 | RhyB sRNA (Other), rpoS (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pCon:RhyB, pLlacO-1:rpoS::gfp
unknown
|
Add to Cart
|
| pHL 1070 | DsrA sRNA (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pCon:DsrA, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1073 | mCherry (Other), GFP (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pCon:ompC::mCherry, pLlacO-1:sodB::gfp
unknown
Tag
/ Fusion Protein- ompC (target mRNA) (N terminal on insert)
|
Add to Cart
|
| pHL 1075 | fhlA (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
unknown
Tag
/ Fusion Protein- mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 1148 | OxyS sRNA (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pCon:OxyS, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1158 | RhyB sRNA (Other), DsrA sRNA (Other), ompC (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1303 | sodB (Other), ompC (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
unknown
Tag
/ Fusion Protein- mCherry (C terminal on insert)
|
Add to Cart
|
| pHL 1394 | RhyB sRNA (Other), T7(st3) (133bps) (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1405 | RhyB sRNA (Other), DsrA sRNA (Other), hfq (Other), sodB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLtetO-1, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
unknown
|
Add to Cart
|
| pHL 1410 | DsrA sRNA (Other), ompC (Other), rpoS (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pCon, pLlacO-1 |
Lim
| Disruption of small RNA signaling caused by competition for Hfq.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.
|
pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
unknown
|
Add to Cart
|
| pHL 342 | mCherry (Synthetic), araC (Other), ompC (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1, Plar |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp
unknown
|
Add to Cart
|
| pHL 1023 | mCherry (Synthetic), gfp (st7) (Synthetic) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Direct comparison of small RNA and transcription factor signaling.
Nucleic Acids Res. 2012 May 22.
|
PLtetO-1:mCherry, PLlacO-1:gfp (st7)
unknown
|
Add to Cart
|
| pHL 246 | GFP (Other) | Bacterial Expression, Synthetic Biology | |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1
unknown
Tag
/ Fusion Protein- fimS (off orientation) (N terminal on insert)
|
Add to Cart
|
| pHL 636 | fimE (Other), fimB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
unknown
|
Add to Cart
|
| pHL 651 | LacIq (Other), tetR (Other) | Bacterial Expression, Synthetic Biology | pCon |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).
pZA32MCS1 (Lutz and Bujard, 1997).
|
Add to Cart
|
| pHL 1277 | GFP (Other), mCherry (Synthetic) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1
unknown
|
Add to Cart
|
| pHL 1278 | mCherry (Synthetic), GFP (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1
unknown
Tag
/ Fusion Protein- RBS(st7) (N terminal on insert)
|
Add to Cart
|
| pHL 1454 | fimB (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1 |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
unknown
|
Add to Cart
|
| pHL 1903 | GFP (Other) | Bacterial Expression, Synthetic Biology | fimS |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1
unknown
|
Add to Cart
|
| pHL 1904 | GFP (Other) | Bacterial Expression, Synthetic Biology | fimS |
Lim
|
Lim Lab plasmids
(unpublished)
|
AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1
unknown
|
Add to Cart
|
| pHL 1915 | GFP (Other) | Bacterial Expression, Synthetic Biology | pLlacO-1 and fimS fusion |
Lim
|
Lim Lab plasmids
(unpublished)
|
AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
unknown
|
Add to Cart
|
| pHL 1916 | fimB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1 |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1
unknown
|
Add to Cart
|
| pHL 1917 | mCherry (Synthetic), fimB (Other) | Bacterial Expression, Synthetic Biology | pCon, pLlacO-1 |
Lim
| Modulating the frequency and bias of stochastic switching to control phenotypic variation.
Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
|
AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
unknown
Tag
/ Fusion Protein- RBS(st7) (N terminal on insert)
fimB b4312, ECK4303, pil
|
Add to Cart
|
| pHL 1977 | fimB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
AmpR + PLtetO-1::RBS (st3) fimB::Asp terminator + ColE1
unknown
|
Add to Cart
|
| pHL1506 | csrA (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PLlacO-1:RBS(st7):csrA
unknown
|
Add to Cart
|
| pHL1529 | GFP (Other), tetR, csrA (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
unknown
Tag
/ Fusion Protein- glgC leader contains -61 to +8 relative to the start codon (N terminal on insert)
|
Add to Cart
|
| pHL1575 | csrB (Other), csrD (Other) | Bacterial Expression, Synthetic Biology | PconNoHind, pLtetO-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PconNoHind:csrB, PLtetO-1:RBS(st3):csrD
unknown
|
Add to Cart
|
| pHL1590 | csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:RBS(st3):csrA
unknown
|
Add to Cart
|
| pHL1722 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- glgC leader (N terminal on insert)
|
Add to Cart
|
| pHL1724 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- pgaAleadFull (N terminal on insert)
|
Add to Cart
|
| pHL1726 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- hfqlead (N terminal on insert)
|
Add to Cart
|
| pHL1745 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- cstAlead (N terminal on insert)
|
Add to Cart
|
| pHL1746 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- ycdTlead (N terminal on insert)
|
Add to Cart
|
| pHL1747 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
unknown
|
Add to Cart
|
| pHL1756 | GFP (Other), tetR (Other) | Bacterial Expression, Synthetic Biology | pConNoHindM12, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR
unknown
Tag
/ Fusion Protein- glgC leader (N terminal on insert)
|
Add to Cart
|
| pHL1757 | GFP (Other), tetR (Other) | Bacterial Expression, Synthetic Biology | PconNoHindM2, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR
unknown
|
Add to Cart
|
| pHL1790 | mCherry (Other), csrB (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
unknown
|
Add to Cart
|
| pHL1801 | GFP (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1 |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PLlacO-1:RBS(st7):gfp
unknown
Tag
/ Fusion Protein- RBS(st7) (N terminal on insert)
|
Add to Cart
|
| pHL1853 | GFP (Other), tetR (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
unknown
Tag
/ Fusion Protein- glgC leader (N terminal on insert)
|
Add to Cart
|
| pHL1855 | GFP (Other), tetR (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLlac0-1, pConNoHind, pConNoHind |
Lim
| Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation.
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22.
|
PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA
unknown
|
Add to Cart
|
| pHL1914 | GFP (Other), tetR (Other) | Bacterial Expression, Synthetic Biology | PconNoHindM2, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
unknown
Tag
/ Fusion Protein- hfqlead (N terminal on insert)
|
Add to Cart
|
| pHL1928 | mCherry (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
unknown
Tag
/ Fusion Protein- Agn43RBS (C terminal on insert)
|
Add to Cart
|
| pHL1940 | cstA (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pLtetO-1, pLlacO-1 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
unknown
|
Add to Cart
|
| pHL1947 | GFP (Other), tetR (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pConNoHindM12, pConNoHind, pConNoHindM8 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrA
unknown
Tag
/ Fusion Protein- glgC leader (N terminal on insert)
|
Add to Cart
|
| pHL1948 | GFP (Other), tetR (Other), csrA (Other) | Bacterial Expression, Synthetic Biology | pConNoHindM12, pConNoHind, pConNoHindM10 |
Lim
|
Lim Lab plasmids
(unpublished)
|
PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA
unknown
|
Add to Cart
|
| Pveg | veg promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | veg |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Very strong constitutive promoter
pSB1C3
|
Add to Cart
|
| PliaG | LiaG promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Constitutive promoter
pSB1C3
|
Add to Cart
|
| PlepA | lepA promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Strong constitutive promoter
pSB1C3
|
Add to Cart
|
| J23101 | J23101 promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | J23101 |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Very weak constitutive promoter
pSB1C3
|
Add to Cart
|
| PliaI | liaI promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | liaI |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Bacitracin-inducible promoter
pSB1C3
|
Add to Cart
|
| PxylA | xylA promoter (Other) | Synthetic Biology ; Bacillus BioBrick Box | xylA |
Mascher
| The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
|
Xylose-inducible promoter
pSB1A3
|
Add to Cart
|
| CMVp-dCas9-3xNLS-VP64 (Construct 1) | dCas9 (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV/hUBC |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.
pFUGw (Addgene id: 25870)
|
Add to Cart
|
| PGK1p-Csy4-pA (Construct 2) | Csy4/Cas6 (Homo sapiens) | Mammalian Expression, Synthetic Biology | CMV |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Expresses Csy4 in Mammalian cells for site-specific RNA processing
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| P1-EYFP-pA (Construct 5) | EYFP (Homo sapiens) | Mammalian Expression, Synthetic Biology | Minimal Ade MLP |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Synthetic promoter responsive to gRNA1
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| P2-ECFP-pA (Construct 6) | ECFP (Homo sapiens) | Mammalian Expression, Synthetic Biology | Minimal Ade MLP |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Synthetic promoter responsive to gRNA2
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22) | ECFP (Homo sapiens) | Mammalian Expression, Synthetic Biology | CMV |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
CFP reporter along with FF4 miRNA binding sites and 28 nt sequences
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| CMVp-dsRed2-Triplex-28-gRNA1-28-pA | dsRed2 (Homo sapiens) | Mammalian Expression, Synthetic Biology | CMV |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA | dsRed2 (Homo sapiens) | Mammalian Expression, Synthetic Biology | CMV |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA | dsRed2 (Homo sapiens) | Mammalian Expression, Synthetic Biology | CMV |
Lu
| Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
|
Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
pGL2-Luc (Addgene #26280)
|
Add to Cart
|
| pTK095 | SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
SIN
|
Add to Cart
|
| pTK101 | SGP(14) mKate-G8-L7Ae-PEST 4xFF4 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4
SIN
|
Add to Cart
|
| pTK105 | SGP(14) 2xK-turn EYFP-PEST | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST
SIN
|
Add to Cart
|
| pTK194 | SGP XbaI attB1 Kozak mKate opal attB2 AscI | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
VEE
|
Add to Cart
|
| pTK295 | SGP(14) 2xK-turn Kozak EGFP | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP
SIN
|
Add to Cart
|
| pTK296 | SGP(14) 2xK-turn Kozak EGFP-PEST | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST
SIN
|
Add to Cart
|
| pTK297 | SGP(14) Kozak mKate-G8-L7Ae 4xFF4 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4
SIN
|
Add to Cart
|
| pTK298 | SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4
SIN
|
Add to Cart
|
| pTK299 | SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4
SIN
|
Add to Cart
|
| pTK312 | SGP XbaI attB1 Kozak EGFP ochre attB2 AscI | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
SIN
|
Add to Cart
|
| pTK313 | SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
VEE
|
Add to Cart
|
| pTK317 | SGP(16) 2xK-turn Kozak EGFP attB2 AscI | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
VEE
|
Add to Cart
|
| pTK331 | SGP(16) 2xK-turn Kozak EGFP attB2 AscI | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
VEE
|
Add to Cart
|
| pTK332 | SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
SIN
|
Add to Cart
|
| pTK333 | SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5 | | |
Weiss
| Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
|
SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
SIN
|
Add to Cart
|
| pTHSSd_1 | Splitposon w/ KanR, LacI & PTac (Synthetic) | Bacterial Expression | |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
Splitposon in KanR ColE1 backbone
pSB1C3
|
Add to Cart
|
| pTHSSd_8 | sfGFP (Synthetic) | Bacterial Expression | PT7 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PT7 GFP reporter
pZS2
|
Add to Cart
|
| pTHSSd_9 | sfGFP (Synthetic) | Bacterial Expression | PT3 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PT3 GFP reporter
pZS2
|
Add to Cart
|
| pTHSSd_10 | sfGFP (Synthetic) | Bacterial Expression | PK1F |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PK1F GFP reporter
pZS2
|
Add to Cart
|
| pTHSSd_11 | sfGFP (Synthetic) | Bacterial Expression | PCCG |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PCGG GFP reporter
pZS2
|
Add to Cart
|
| pTHSSd_14 | Three-fragment T7* RNA polymerase (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
Triple split (at 67, 601-SZ)
pSB3C5
|
Add to Cart
|
| pTHSSd_18 | Four-fragment T7* RNA polymerase (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
Quad split (at 61, 179, 601-SZ)
pSB3C5
|
Add to Cart
|
| pTHSSd_23 | T7 sigma fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac T7 sigma fragment expression
pSB3C5
|
Add to Cart
|
| pTHSSd_24 | T3 sigma fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac T3 sigma fragment expression
pSB3C5
|
Add to Cart
|
| pTHSSd_25 | K1FR sigma fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac K1FR sigma fragment expression
pSB3C5
|
Add to Cart
|
| pTHSSd_26 | CGG sigma fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac CGG sigma fragment expression
pSB3C5
|
Add to Cart
|
| pTHSSd_27 | null fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac null fragment (sigma CGG Y639A) expression
pSB3C5
|
Add to Cart
|
| pTHSSd_29 | alpha fragment (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac alpha fragment expression
pSB3C5
|
Add to Cart
|
| pTHSSd_34 | sfGFP (Synthetic) | Bacterial Expression | PTac |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
PTac GFP expression
pSB3C5
|
Add to Cart
|
| pTHSSd_38 | core fragment (Synthetic) | Bacterial Expression | PJ23105 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
High core fragment expression (high resource allocator)
pBACr
|
Add to Cart
|
| pTHSSd_39 | core fragment (Synthetic) | Bacterial Expression | PJ23105 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
Low core fragment expression (low resource allocator)
pBACr
|
Add to Cart
|
| pTHSSd_40 | core fragment lacking SynZIP (Synthetic) | Bacterial Expression | PJ23105 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
High core fragment expression without SynZIP
pBACr
|
Add to Cart
|
| pTHSSd_41 | T7* RNAP (Synthetic) | Bacterial Expression | PJ23105 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
High full length T7 RNAP* expression
pBACr
|
Add to Cart
|
| pTHSSd_42 | beta core fragment (Synthetic) | Bacterial Expression | PJ23105 |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
beta core fragment expression
pBACr
|
Add to Cart
|
| pTHSSd_44 | T7 sigma fragment (Synthetic) | Bacterial Expression | Pcon sigma |
Voigt
| A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.
Mol Syst Biol. 2014 Jul 30;10(7):742.
|
constitutive expression of T7 sigma fragment
pSB1C3
|
Add to Cart
|
| Gal4pBS2-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| Gal4pBS1-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| Gal4pBS12-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter
p416
|
Add to Cart
|
| Gal4pBS24-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter
p416
|
Add to Cart
|
| UASGAL-CU2-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.
p416
|
Add to Cart
|
| Gal4pBS4-Pleum | promoter | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| Gal4pBS3-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| UASGAL-CU1-Pleum | promoter | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.
p416
|
Add to Cart
|
| Gal4pBS13-Pleum | promoter | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| UASGAL-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| Gal4pBS34-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| UASGAL-PCYC | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| PGAL | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains full length native galactose promoter
p416
|
Add to Cart
|
| UASGAL-Pgal | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter
p416
|
Add to Cart
|
| Gal4pBS134-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
p416
|
Add to Cart
|
| UASgal-A9-Pleum | promoter (Saccharomyces cerevisiae) | Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct | |
Alper
| Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.
Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
|
Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
p416
|
Add to Cart
|
| pReporter_2 | Reporter_2 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_3 | Reporter_3 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_4 | Reporter_4 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_5 | Reporter_5 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_7 | Reporter_7 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_8 | Reporter_8 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_9 | Reporter_9 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_10 | Reporter_10 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_11 | Reporter_11 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pReporter_12 | Reporter_12 (Synthetic) | Bacterial Expression | BBa_J23119 |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
unknown
|
Add to Cart
|
| pIntegrase_2 | Integrase_2 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_3 | Integrase_3 (Synthetic) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_4 | Integrase_4 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_5 | Integrase_5 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_7 | Integrase_7 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_8 | Integrase_8 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_9 | Integrase_9 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_10 | Integrase_10 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_11 | Integrase_11 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_12 | Integrase_12 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pIntegrase_13 | Integrase_13 (Other) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
unknown
|
Add to Cart
|
| pMemoryArray | MemoryArray (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.
unknown
|
Add to Cart
|
| pCis_2+5 | Cis_2+5 (Synthetic) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
a 2-integrase plasmid
unknown
|
Add to Cart
|
| pCis_7+8 | Cis_7+8 (Synthetic) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
a 2-integrase plasmid
unknown
|
Add to Cart
|
| pCis_7+8+10 | Cis_7+8+10 (Synthetic) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
a 3-integrase plasmid
unknown
|
Add to Cart
|
| pCis_2+7+8+5 | Cis_2+7+8+5 (Synthetic) | Bacterial Expression | pBAD |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
a 4-integrase plasmid
unknown
|
Add to Cart
|
| pAND_Int2 | AND_Int2 (Synthetic) | Bacterial Expression | PPhlF |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
A transcriptional AND circuit constructed by layering NOR and NOT gates
unknown
|
Add to Cart
|
| pAND_Reporter | AND_Reporter (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
AND gate reporter
unknown
|
Add to Cart
|
| pAND_yfp | AND_yfp (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
used with pSpec to generate an AND gate without memory
unknown
|
Add to Cart
|
| pSpec | Spec (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
used with pAND_YFP to generate an AND gate without memory
unknown
|
Add to Cart
|
| pCas_2+5 | Cas_2+5 (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2
unknown
|
Add to Cart
|
| pCas_5+7_gfp | Cas_5+7_gfp (Synthetic) | Bacterial Expression | |
Voigt
| Permanent genetic memory with >1-byte capacity.
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
|
encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.
unknown
|
Add to Cart
|
| pTara:500 | T7 RNAP (Other) | Bacterial Expression, Synthetic Biology | PBAD |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara | N Terminal fragment of T7 RNAP split betwen 179-180 (Synthetic), N Terminal fragment of T7 RNAP split betwen 179-180 (Other) | Bacterial Expression | PBAD |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-N | Residues 1-179 of T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | PBAD |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-C | Residues 180-880 of T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | PBAD |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-Q758C | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-R756S | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-N748D | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSara-R756K | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
pBAD33
|
Add to Cart
|
| pTSlb-wt | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
pBAD33
|
Add to Cart
|
| pTSlb-Q758C | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
pBAD33
|
Add to Cart
|
| pTSlb-R756S | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
pBAD33
|
Add to Cart
|
| pTSlb-R756K | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
pBAD33
|
Add to Cart
|
| pTSlb-N748D | Residues 1-179 of split T7 RNAP (Synthetic), Residues 180-880 of split T7 RNAP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.
pBAD33
|
Add to Cart
|
| pET28:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
pET28 driving expression of GFP, biobrick part Bba_E0040
pET-28b
|
Add to Cart
|
| pET:GACT:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.
pET-28b
|
Add to Cart
|
| pET:GACG:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.
pET-28b
|
Add to Cart
|
| pET:GCAT:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.
pET-28b
|
Add to Cart
|
| pET:CACT:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.
pET-28b
|
Add to Cart
|
| pET:CCCT:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.
pET-28b
|
Add to Cart
|
| pET:ACAT:GFP | GFP | Bacterial Expression, Synthetic Biology | |
Bennett
| Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants.
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 10.1073/pnas.1220157110. Epub 2013 Mar 11.
|
A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.
pET-28b
|
Add to Cart
|
| pZS1[RbsR-L] | RbsR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.
pZS5
|
Add to Cart
|
| pZS1[FruR-L] | FruR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.
pZS5
|
Add to Cart
|
| pzs1[GalS-L] | GalS-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.
pZS5
|
Add to Cart
|
| pZS1[LacI-L] | LacI (Other) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PI driving expression dimeric LacI, the Lactose inducible repressor.
pZS5
|
Add to Cart
|
| pZS1[RbsR-T] | RbsR-T (Other) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.
pZS5
|
Add to Cart
|
| pZS1[FruR-T] | FruR-T (Other), FruR-T (Other) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
pZS5
|
Add to Cart
|
| pZS1[GalS-T] | GalS-T (Other), GalS-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.
pZS5
|
Add to Cart
|
| pZS1[TreR-T] | TreR-T (Other), GalS-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.
pZS5
|
Add to Cart
|
| pZS1[Iq:LacI-T] | LacI-T (Other) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.
pZS5
|
Add to Cart
|
| pZS1[RbsR-L,LacI-L] | RbsR-L (Synthetic), Dimeric LacI (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.
pZS5
|
Add to Cart
|
| pZS1[GalS-L,LacI-L] | GalS-L (Synthetic), wt LacI (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.
pZS5
|
Add to Cart
|
| pZS1[TreR-L,LacI-L] | TreR-L (Synthetic), Dimeric LacI (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PI driving expression TreR-L, and PI driving expression of LacI-L
pZS5
|
Add to Cart
|
| pZS1[FruR-L,RbsR-L] | FruR-L (Synthetic), RbsR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression TreR-L, and PIq driving expression of RbsR-L
pZS5
|
Add to Cart
|
| pZS1[GalS-L,RbsR-L] | GalS-L (Synthetic), RbsR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.
pZS5
|
Add to Cart
|
| pZS1[GalS-L,FruR-L] | GalS-L (Synthetic), FruR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-L, and PIq driving expression of FruR-L.
pZS5
|
Add to Cart
|
| pZS1[GalS-L,TreR-L] | GalS-L (Synthetic), TreR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-L, and PI driving expression of TreR-L.
pZS5
|
Add to Cart
|
| pZS1[RbsR-L,TreR-L] | RbsR-L (Synthetic), TreR-L (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-L, and PI driving expression of TreR-L.
pZS5
|
Add to Cart
|
| pZS1[RbsR-T,LacI-T] | RbsR-L (Synthetic), Dimeric LacI with the TAN DBD (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-T, and PI driving expression of TreR-L.
pZS5
|
Add to Cart
|
| pZS1[FruR-T,LacI-T] | FruR-T (Synthetic), Dimeric LacI with the TAN DBD (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression FruR-T, and PI driving expression of LacI-T.
pZS5
|
Add to Cart
|
| pZS1[TreR-T,LacI-T] | TreR-T (Synthetic), Dimeric LacI with the TAN DBD (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PI driving expression TreR-T, and PI driving expression of LacI-T.
pZS5
|
Add to Cart
|
| pZS1[FruR-T,TreR-T] | FruR-T (Synthetic), TreR-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression FruR-T, and PIq driving expression of TreR-T.
pZS5
|
Add to Cart
|
| pZS1[GalS-T,RbsR-T] | GalS-T (Synthetic), GalS-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.
pZS5
|
Add to Cart
|
| pZS1[GalS-T,TreR-T] | GalS-T (Synthetic), TreR-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression GalS-T, and PI driving expression of TreR-T.
pZS5
|
Add to Cart
|
| pZS1[RbsR-T,TreR-T] | RbsR-L (Synthetic), TreR-T (Synthetic) | Bacterial Expression, Synthetic Biology | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PIq driving expression RbsR-T, and PI driving expression of TreR-T.
pZS5
|
Add to Cart
|
| pZM3[I:FruR-L,I:RbsR-L] | FruR-L (Synthetic), RbsR-L (Synthetic) | | |
Bennett
| Modular, Multi-Input Transcriptional Logic Gating with Orthogonal LacI/GalR Family Chimeras.
ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. Epub 2014 Jul 28.
|
Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.
pET28-b
|
Add to Cart
|
| pcrRNA.ind | | Bacterial Expression, CRISPR, Synthetic Biology | pBAD |
Beisel
| Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression.
Nucleic Acids Res. 2014 Oct 17. pii: gku971.
|
Arabinose-inducible repeat-only E. coli CRISPR array
pBAD18
|
Add to Cart
|
| pcDNA-dCas9-HA | S.pyogenes dCas9 with c-terminal HA tag (Synthetic) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
pcDNA3.1
|
Add to Cart
|
| pcDNA-dCas9-FLp300 | S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (D1399Y) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (1645/1646 RR/EE) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (C1204R) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (Y1467F) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (1396/1397 SY/WW) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) | S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
pcDNA3.1
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pcDNA3.3-Nm-dCas9-p300 Core | Nm-dCas9-p300 Core (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
pcDNA3.3
EP300 KAT3B, MKHK2, RSTS2, p300
|
Add to Cart
|
| pZDonor-Nm-Cas9-gRNA-hU6 | | Mammalian Expression, CRISPR, Synthetic Biology | human U6 |
Gersbach
| Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers.
Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.
|
empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA
pZdonor
|
Add to Cart
|
| pBW313lux-hrpR | F2620-rbs33-hrpR-ter (Synthetic) | Bacterial Expression | plux |
Buck
| Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology.
Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
|
pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))
pSB3K3
|
Add to Cart
|
| pBW400hrpL-gfp | hrpL-rbs30-gfp-ter (Synthetic) | Bacterial Expression | HrpL |
Buck
| Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology.
Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
|
pBW400hrpL-gfp
pSB4A3
|
Add to Cart
|
| pBW412hrpL-cIgfp | HrpL-rbs32-CI-LVA-t-plam-30gfp-t (Synthetic) | Bacterial Expression | HrpL |
Buck
| Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology.
Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
|
pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter
pSB4A3
|
Add to Cart
|
| pBW414hrpL-cIgfp | HrpL-rbs34-CI-LVA-t-plam-30gfp-t (Synthetic) | Bacterial Expression | HrpL |
Buck
| Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology.
Nat Commun. 2011 Oct 18;2:508. doi: 10.1038/ncomms1516.
|
pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter
pSB4A3
|
Add to Cart
|
| pAN-PTet-dCas9 | dCas9 (Other) | Bacterial Expression, CRISPR | Ptet |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.
unknown
|
Add to Cart
|
| pAN-PTet-YFP | YFP (Other) | Bacterial Expression | Ptet |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.
unknown
|
Add to Cart
|
| pAN-PBAD-YFP | YFP (Other) | Bacterial Expression | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.
unknown
|
Add to Cart
|
| pAN-PA1-RFP | mRFP (Other) | Bacterial Expression | pA1 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
unknown
|
Add to Cart
|
| pAN-PA2-RFP | mRFP (Other) | Bacterial Expression | pA2 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
unknown
|
Add to Cart
|
| pAN-PA3-RFP | mRFP (Other) | Bacterial Expression | pA3 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
unknown
|
Add to Cart
|
| pAN-PA4-RFP | mRFP (Other) | Bacterial Expression | pA4 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
unknown
|
Add to Cart
|
| pAN-PA5-RFP | mRFP (Other) | Bacterial Expression | pA5 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A1NT | A1NT (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A1NT sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A1T | A1T sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A1T sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A2NT | A2NT sgRNA | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A2NT sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A2T | A2T sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A2T sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A3NT | A3NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A3NT sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A3T | A3T sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A3T sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A4NT | A4NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A4NT sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A4T | A4T sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A4T sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A5NT | A5NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A5NT sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-A5T | A5T sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of A5T sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-scramble | scramble sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of scramble sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-PBAD-sgRNA-VR | VR sgRNA (Synthetic) | Bacterial Expression, CRISPR | pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
expression of VR sgRNA from the arabinose-inducible promoter
unknown
|
Add to Cart
|
| pAN-NOR | A2NT sgRNA (Synthetic), A2NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | PPhlF, pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
synthetic circuit: NOR gate
unknown
|
Add to Cart
|
| pAN-OR | A2NT sgRNA (Synthetic), A4NT sgRNA (Synthetic), A2NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | PPhlF, PA2, pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
synthetic circuit: OR gate
unknown
|
Add to Cart
|
| pAN-AND | A1NT sgRNA (Synthetic), A4NT sgRNA (Synthetic), A2NT sgRNA (Synthetic), A1NT (Synthetic) | Bacterial Expression, CRISPR | pA4, PPhlF, pBAD, PA2 |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
synthetic circuit: AND gate
unknown
|
Add to Cart
|
| pAN-OR-MalT-3NT | A2NT sgRNA (Synthetic), Malt-3NT sgRNA (Synthetic), A2NT sgRNA (Synthetic) | Bacterial Expression, CRISPR | PPhlF, PA2, pBAD |
Voigt
| Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks.
Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
|
synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome
unknown
|
Add to Cart
|
| pJZC625 | sgRNA +1x MS2, Pol II promoter with ribozyme cleavage | Yeast Expression | pAdh |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells
pSV616
|
Add to Cart
|
| pJZC32 | sgRNA, MCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC25 | sgRNA + 1x MS2 binding module, MCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC33 | sgRNA + 2x MS2 binding module, MCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC34 | sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC41 | sgRNA, PCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC39 | sgRNA + 1x PP7, mCherry | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1x PP7 with mCherry for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC40 | sgRNA + 2x PP7, mCherry | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 2x PP7 with mCherry for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC101 | sgRNA, COM-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC48 | sgRNA + 1x COM binding module, COM-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1x COM with COM-VP64 effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC77 | sgRNA, COM-KRAB | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC78 | sgRNA + 1x COM binding module, COM-KRAB | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1x COM with COM-KRAB effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC73 | sgRNA, COM-KRAB | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC74 | sgRNA + 1x COM binding module, COM-KRAB | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1x COM with COM-KRAB effector for mammalian cells
MP177_U6 (derived from pSico)
|
Add to Cart
|
| pJZC116 | sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64 | Mammalian Expression, Lentiviral | U6, CMV |
Qi
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
MP177_U6 (derived from pSico)
|
Add to Cart
|
| MXS_PGK | PGK Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PGK
MXS_chaining vector
|
Add to Cart
|
| MXS_minPGK | Minimal PGK Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with minPGK
MXS_chaining vector
|
Add to Cart
|
| MXS_PGKenhancer | PGK enhancer (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PGKenhancer
MXS_chaining vector
|
Add to Cart
|
| MXS_CMV | CMV Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CMV
MXS_chaining vector
|
Add to Cart
|
| MXS_minCMV | Minimal CMV Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with minCMV
MXS_chaining vector
|
Add to Cart
|
| MXS_CMVenhancer | CMV enhancer (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CMVenhancer
MXS_chaining vector
|
Add to Cart
|
| MXS_CAG | CAG Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CAG
MXS_chaining vector
|
Add to Cart
|
| MXS_EF1a | EF1a Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with EF1a
MXS_chaining vector
|
Add to Cart
|
| MXS_Tet | Tetracycline inducible promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with Tet
MXS_chaining vector
|
Add to Cart
|
| MXS_BiTet | Bidirectional tetracycline inducible promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with BiTet
MXS_chaining vector
|
Add to Cart
|
| MXS_bGpA | beta-Globin poly A (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with bGpA
MXS_chaining vector
|
Add to Cart
|
| MXS_bGHpA | Bovine growth hormone poly A (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_SV40pA | SV40 poly A (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with SV40pA
MXS_chaining vector
|
Add to Cart
|
| MXS_CreERT2 | Tamoxifen inducible Cre recombinase (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CreERT2
MXS_chaining vector
|
Add to Cart
|
| MXS_rtTA3 | Reverse tetracycline transactivator (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with rtTA3
MXS_chaining vector
|
Add to Cart
|
| MXS_tTA2 | Tetracycline transactivator (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with rTA2
MXS_chaining vector
|
Add to Cart
|
| MXS_loxP | loxP site (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with loxP
MXS_chaining vector
|
Add to Cart
|
| MXS_linker | Linker 12AA Gly-/Ser-rich (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with linker
MXS_chaining vector
|
Add to Cart
|
| MXS_PEST2D | Destabilization tag (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PEST2D
MXS_chaining vector
|
Add to Cart
|
| MXS_CMV::CreERT2-bGHpA | cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CMV::CreERT2-bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_PGK::CreERT2-bGHpA | cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PGK::CreERT2-bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_CMV::rtTA3-bGHpA | cassette containing the reverse tetracycline transactivator with CMV promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CMV::rtTA3-bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_PGK::rtTA3-bGHpA | cassette containing the reverse tetracycline transactivator with PGK promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PGK::rtTA3-bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_CMV::tTA2-bGHpA | cassette containing the tetracycline transactivator with CMV promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with CMV::tTA2-bGHpA
MXS_chaining vector
|
Add to Cart
|
| MXS_PGK::tTA2-bGHpA | cassette containing the tetracycline transactivator with PGK promoter (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with PGK::tTA2-bGHpA
MXS_chaining vector
|
Add to Cart
|
| ChromosomesMembranesandCytoskeleton | CMV::H2B-TagBFPx3-bGHpA (Synthetic), CMV::Lyn-Ceruleanx3-bGHpA (Synthetic), CMV::mCherryx3-linker-betaActin-bGHpA (Synthetic), CMV::Citrinex3-linker-alphaTubulin-bGHpA (Synthetic) | Synthetic Biology | |
Neveu
| MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.
|
MXS_chaining vector with 4 cassettes
MXS_chaining vector
|
Add to Cart
|
| pRPR1(1xTetO)_gRNA_handle_RPR1t | | Yeast Expression | RPR1 |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
aTc inducible gRNA expression vector
pRS425
|
Add to Cart
|
| SP-dCas9-VPR | SP-dCas9-VPR (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
pcDNA3.3 TOPO
|
Add to Cart
|
| M_ST1n_VPR | ST1-dCas9-VPR (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology | CMV |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector
pcDNA3.3 TOPO
|
Add to Cart
|
| PB-TRE-dCas9-VPR | dCas9-VPR (Homo sapiens) | Mammalian Expression, CRISPR, Synthetic Biology ; Piggybac | TRE |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
SP-dCas9-VPR with doxycycline-inducible expression
PB-TRE
|
Add to Cart
|
| pAG414GPD-dCas9-VPR | dCas9-VPR (Saccharomyces cerevisiae) | Yeast Expression, CRISPR, Synthetic Biology | GPD |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector
pAG414GPD
|
Add to Cart
|
| pAWG-dCas9-VPR | SP-dCas9-VPR (Drosophila melanogaster) | Insect Expression, CRISPR, Synthetic Biology | act5c |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector
pAWG
|
Add to Cart
|
| AEB-No-aptamer-controlmRFP1 | No-aptamer control mRNA (Synthetic) | Synthetic Biology | J23100 |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.
pFTV1
|
Add to Cart
|
| AEB-F12 | F12 fluoride riboswitch (Synthetic) | Synthetic Biology | J23100 |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.
pFTV1
|
Add to Cart
|
| AEB-Theo45 | Theo45 theophylline riboswitch (Synthetic) | Synthetic Biology | J23100 |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a theophylline binding riboswitch, controlling the translation initaition rate of mRFP1 gene.
pFTV1
|
Add to Cart
|
| AEB-No-aptamer-control -- AEB3mRFP1 | No-aptamer control mRNA (Synthetic) | Synthetic Biology | AEB3 |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.
pFTV1
|
Add to Cart
|
| AEB-DNT3 | DNT3 DNT riboswitch (Synthetic) | Synthetic Biology | AEB3 |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene.
pFTV1
|
Add to Cart
|
| AEB-No-aptamer-controlluciferase | No-aptamer control mRNA (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.
pFTV1
|
Add to Cart
|
| AEB-Dopa5 | Dopa5 dopamine riboswitch (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a dopamine binding riboswitch, controlling the translation initaition rate of luciferase gene.
pFTV1
|
Add to Cart
|
| AEB-T4-2 | T4-2 thyroxine riboswitch (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.
pFTV1
|
Add to Cart
|
| Mishler2014-No-aptamer-controlluciferase-pUC19 | No-aptamer control mRNA (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.
pUC19
|
Add to Cart
|
| AEB-TMR7pUC19 | TMR7 TMR riboswitch (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.
pUC19
|
Add to Cart
|
| AEB-TMR10pUC19 | TMR10 TMR riboswitch (Synthetic) | Synthetic Biology | tac |
Salis
| Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
|
This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.
pUC19
|
Add to Cart
|
| pSB700 | | Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology | U6, CAGGS |
Church
| Highly efficient Cas9-mediated transcriptional programming.
Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
|
Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
pLVX
|
Add to Cart
|
| pJexpress414_OmpA171_H1248 | OmpA_H1248 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1248
pJexpress414
|
Add to Cart
|
| pCYC1m_yeGFP | yeast enhanced GFP (Saccharomyces cerevisiae) | Yeast Expression | pCYC1 |
Lu
| Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
ACS Synth Biol. 2013 Sep 11.
|
encodes yeast enhanced GFP under control of minimal pCYC1 promoter
pRS406
|
Add to Cart
|
| XW08 | OR74A quinic acid utilization activator (qa-1F) (Other) | Worm Expression | Punc-4 |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
Punc-4-QF-SL2-mcherry-3'UTR-unc-54
pSM
|
Add to Cart
|
| XW09 | Neurospora crassa OR74A repressor (qa-1S) (Other) | Worm Expression | Punc-4c |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
Punc-4c-QS-SL2-mcherry3'UTR-unc-54
pSM
|
Add to Cart
|
| XW17 | OR74A quinic acid utilization activator (qa-1F) (Other) | Worm Expression | |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
pSM
|
Add to Cart
|
| XW20 | OR74A quinic acid utilization activator (qa-1F) (Other) | Worm Expression | |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)
pSM
|
Add to Cart
|
| XW21 | Neurospora crassa OR74A repressor (qa-1S) (Other) | Worm Expression | Punc-4c |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)
pSM
|
Add to Cart
|
| XW83 | OR74A quinic acid utilization activator (qa-1F) (Other) | Worm Expression | |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
MosSCI-Pmig-13::QF::SL2::mCherry
CM290 (a derivative of pCFJ178)
|
Add to Cart
|
| XW99 | Neurospora crassa OR74A repressor (qa-1S) (Other) | Worm Expression | |
Shen
| Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
|
MosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I.
pCFJ352
|
Add to Cart
|
| Burden Monitor lambda version | GFP | | |
Ellis
| Quantifying cellular capacity identifies gene expression designs with reduced burden.
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
|
The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
pAH63
|
Add to Cart
|
| Burden Monitor phi80 version | GFP | | |
Ellis
| Quantifying cellular capacity identifies gene expression designs with reduced burden.
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
|
The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell
pAH153
|
Add to Cart
|
| pInt-ts | lambda integrase | | |
Ellis
| Quantifying cellular capacity identifies gene expression designs with reduced burden.
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
|
The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version
pInt-ts
|
Add to Cart
|
| pAH123 | phi80 integrase | | |
Ellis
| Quantifying cellular capacity identifies gene expression designs with reduced burden.
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
|
The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version
pAH123
|
Add to Cart
|
| pJZC42 | sgRNA + 1XPP7, PCP-VP64 IRES mCherry | Mammalian Expression | U6, CMV |
Lim
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry
pSico
|
Add to Cart
|
| pJZC43 | sgRNA + 2XPP7, PCP-VP64 IRES mCherry | Mammalian Expression | U6, CMV |
Lim
| Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
|
sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry
pSico
|
Add to Cart
|
| pRAV12 | Transcription cassette for 3'-tagged RNAs (Synthetic) | Bacterial Expression, Synthetic Biology | T7 RNA polymerase |
Batey
| A general method for rapid and nondenaturing purification of RNAs.
RNA. 2004 Jun;10(6):988-95.
|
Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
pUC19
|
Add to Cart
|
| pTmaM4 | Thermotoga marítima Ffh-M domain (Other) | Bacterial Expression | T7 RNA polymerase |
Batey
| A general method for rapid and nondenaturing purification of RNAs.
RNA. 2004 Jun;10(6):988-95.
|
Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
pET15b
|
Add to Cart
|
| Pr-deGFP-MGapt | UTR1-deGFP-MGapt-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion
pBEST
|
Add to Cart
|
| Pr1-deGFP-MGapt | UTR1-deGFP-MGapt-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr1 |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion
pBEST
|
Add to Cart
|
| Pr2-deGFP-MGapt | UTR1-deGFP-MGapt-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr2 |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion
pBEST
|
Add to Cart
|
| Pr1-T7RNAP | UTR1-T7RNAP-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr1 |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-Pr1 promoter expressing T7 RNAP
pBEST
|
Add to Cart
|
| Pr2-T7RNAP | UTR1-T7RNAP-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr2 |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-P2 promoter expressing T7 RNAP
pBEST
|
Add to Cart
|
| Pr-deCFP-MGapt | UTR1-deCFP-MGapt-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion
pBEST
|
Add to Cart
|
| PT7-deGFP-MGapt | UTR1-deGFP-MGapt-T7term (Synthetic) | Bacterial Expression, Synthetic Biology | PT7 |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
T7 promoter expressing deGFP-MG aptamer fusion
pIVEX2.3d
|
Add to Cart
|
| Pr-deGFP | UTR1-deGFP-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
Pr promoter expressing deGFP
pBEST
|
Add to Cart
|
| Pr-delRBS-deGFP | deGFP-T500 (Synthetic) | Bacterial Expression, Synthetic Biology | OR2-OR1-Pr |
Murray
| Gene circuit performance characterization and resource usage in a cell-free "breadboard".
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
|
Pr promoter expressing a deGFP that is missing a ribosomal binding site
pBEST
|
Add to Cart
|
| BASIC_6_J23102 | J23102 Anderson Promoter | Bacterial Expression | |
Baldwin
| BASIC: A New Biopart Assembly Standard for Idempotent Cloning Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
ACS Synth Biol. 2015 Apr 16.
|
storage vector for BASIC part: J23102
pjet 1.2
|
Add to Cart
|
| SepOTSβ | SepRS/EF-Sep, 1xtRNA-Sep (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSγ | SepRS9/EF-Sep21, 1xtRNA-Sep (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSδ | SepRS/EF-Sep, 1xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSε | SepRS9/EF-Sep21, 1xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSζ | SepRS/EF-Sep, 2xtRNA-Sep (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSη | SepRS9/EF-Sep21, 2xtRNA-Sep (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSθ | SepRS/EF-Sep, 2xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSι | SepRS9/EFSep21, 2xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSκ | SepRS/EF-Sep, 4xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| SepOTSλ (recommended) | SepRS9/EFSep21, 4xtRNA-Sep-A37 (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system (preferred variant)
pKD
|
Add to Cart
|
| SepOTSν | SepRS9/EF-Sep21, 5xtRNA-Sep (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Phosphoserine orthogonal translation system
pKD
|
Add to Cart
|
| Beta lactamase S68TAG | S68TAG beta lactamase (Synthetic) | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Beta lactamase S68TAG gene
pCRT7/NT-TOPO
|
Add to Cart
|
| SupD | 2xtRNA-SupD | Bacterial Expression, Synthetic Biology | |
Rinehart
| A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
|
Plasmid encoding tRNA-SupD for serine amber suppression
pKD
|
Add to Cart
|
| pSCKbreak12 | CisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,cisRaj12-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj11 | TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR
pSTC1
|
Add to Cart
|
| pSCKtheoRaj12-olsa | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj12-o11 | TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj12 | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic) | Synthetic Biology | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR
pSTC2
|
Add to Cart
|
| pENV8(WT)-GFPuv | wild type environmental cobalamin riboswitch sequence (env8) (Other) | Bacterial Expression, Synthetic Biology | tac |
Batey
| B12 cofactors directly stabilize an mRNA regulatory switch.
Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
|
Cobalamin riboswitch reporter vector for use in E. coli
pBR327
|
Add to Cart
|
| pENV8(GAAA)-GFPuv | kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8) (Other) | Bacterial Expression, Synthetic Biology | tac |
Batey
| B12 cofactors directly stabilize an mRNA regulatory switch.
Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
|
Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
pBR327
|
Add to Cart
|
| pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p) | ClpX | | |
Murray
| Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
|
Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.
pBEST
clpX b0438, ECK0432, JW0428, lopC
|
Add to Cart
|
| pBEST_OR2-OR1-Pr_UTR1-deGFP-T500
BsaI, BbsI-safe (105p) | deGFP | | |
Murray
| Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
|
Derived from 21 with silent
mutations to make BsaI, BbsI
compatible
pBEST
|
Add to Cart
|
| pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p) | tetR-deGFP fusion | | |
Murray
| Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
|
Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector
pBEST
|
Add to Cart
|
| pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p) | deGFP | | |
Murray
| Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
|
Promoter panel
pBEST
|
Add to Cart
|
| pBEST-p15A-Pl-tetO1-deGFP-T500 (122p) | deGFP | | |
Murray
| Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
|
Promoter panel
pBEST
|
Add to Cart
|
| pBEST-p15A-Pl-lacO1-deGFP-T500 (123p) | deGFP | | |
Murray
| |
