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SynBio_Icon_Sensing_and_Signaling.png Synthetic Biology: Sensing and Signaling


Browse Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing.



Sensing and Signaling Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Gene/Insert Vector Type Promoter Tags PI Publication
pAL149pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic BiologyCMV Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL175p!SV40-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL188p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL189p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL190p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL197p!SV40-PakGBD-mCherry (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL198p!SV40-mCherry-WaspGBD (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL204pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL113pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL101pCMV-PIF6APB-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pAL130pCMV-PIF3NT-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
pCph8pCph8Bacterial Expression, Synthetic Biology Voigt Synthetic biology: engineering Escherichia coli to see light. Nature. 2005 Nov 24. 438(7067):441-2.
pHL32 (in cHNL135)Bacterial Expression, Synthetic Biology Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
pJT103PompC-lacZBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pJT104Edge detector delta luxIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pJT105Edge detector delta cIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pEDL3Edge detectorBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
pJT106bred light inverterBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT106b3red light inverter with lacZ outputBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT116cca cluster from Synechocystis PCC6803Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT118pJT118Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pJT122pJT122Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
pET21b-LOV-ipaALOV-ipaA (Synthetic)Bacterial ExpressionT76xHis Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
pET21b-LOV-ipaA L514K L531ELOV-ipaA L514K L531E (Synthetic)Bacterial ExpressionT76xHis Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
pZE-PalkB-GFPGFP, PalkBBacterial Expression, Synthetic BiologyPalkB, PalkB Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
pACYC-PLlambda-alkS(WT)AlkS(WT)Bacterial Expression, Synthetic BiologyPLlambda Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
pACYC-PLlambda-alkSm4-3AlkSm4-3 (Other)Synthetic BiologyPLlambda Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
pCS-PesaRluxPesaR (Other)Bacterial Expression, Synthetic BiologyPesaR Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pCS-PluxIluxPluxI (Other)Bacterial Expression, Synthetic BiologyPluxI Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaRPlac-esaR-terminatorBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-I70V/D91GesaR-I70V/D91GBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-V220AesaR-V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-D91GesaR-D91GBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-I70V/D91G/V220AesaR-I70V/D91G/V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pAC-EsaR-D91G/V220AesaR-D91G/V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pCS-PesaR-DluxPesaR-DBacterial Expression, Synthetic BiologyPesaR-D Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pCS-PesaR-PluxPesaR-PBacterial Expression, Synthetic BiologyPesaR-P Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-DluxPesaR-DBacterial Expression, Synthetic BiologyPesaR-D Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-CluxPesaR-CBacterial Expression, Synthetic BiologyPesaR-C Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pET-PesaR-PluxPesaR-PBacterial Expression, Synthetic BiologyPesaR-P Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-I70V-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-D91G-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pAC-EsaR-I70VesaR-I70VBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
pET-PesaRluxPesaR-luxCDABE-termBacterial Expression, Synthetic BiologyPesaR Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
pTD103aiiAaiiA and LuxRBacterial Expression, Synthetic BiologypLuxssrA (TSAANDENYALAA) Hasty A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753.
pTD103luxI_sfGFPLuxI, sfGFP, and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103aiiA(Cm)aiiA and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103luxI_sfGFP -RsfGFP and luxIBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pTD103aiiA(Cm) -RaiiABacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm45 ndhIIndhII and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm45 sodAsodA LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZSm47a luxR RBSluxRBacterial Expression, Synthetic BiologypArsR Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pZS47a luxI-laa RBSLuxIBacterial Expression, Synthetic BiologypArsR Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
pHR SFFVp PHAkt CeruleanPHAktLentiviral, Synthetic BiologyCerulean Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR SFFVp Phy-mCherry-CAAXPhyB 1-908 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR iSH-YFP-PIFp85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
pHR SFFVp BFP-Erk2Erk2 (Mus musculus)LentiviralTagBFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pHR SFFVp BFP-PIF-SOS2catSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)LentiviralTagBFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pHR SFFVp YFP-PIF-SOS2catSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)LentiviralYFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
pP3-GFPP3 promoter (Other)Bacterial Expression, Synthetic BiologyP3none Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pXylA-agrCA-IagrCA locus (Other)Bacterial Expression, Synthetic BiologyPxylAnone Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pXylA-agrCA-IVagrCA locus (Other)Bacterial Expression, Synthetic BiologyPxylAnone Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pT7-agrBD-IagrBD locus (Other)Bacterial Expression, Synthetic BiologyT7-lacV5 epitope, 6x HIS (not expressed on agrB or agrD) Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
pT7-agrBD-IVagrBD locus (with D29Y mutation in agrD) (Other)Bacterial Expression, Synthetic BiologyT7-lacV5 epitope, 6x HIS (not expressed on agrB or agrD) Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
tTA_BFPtTA transcription factor fused to enhanced blue fluorescent protein (Synthetic)Mammalian ExpressionCMVBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
pBI-MCS-EYFPenhanced yellow fluorescent protein (Synthetic)Mammalian ExpressionCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS G_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_TEVMESA protease chain with mCherry ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
CD4_LD 0_CS G_tTA-BFPMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
CD4_LD 0_TEVMESA protease chain with CD4 ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_CS G_tTA-BFPMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_TEVMESA protease chain with dTomato ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS A_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
mCherry_LD 0_CS M_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
dTomato_LD 0_CS M_tTA-BFPMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FRB_LD 0_CS G_tTA-BFPMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FKBP_LD 0_TEVMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic)Mammalian Expression, AAVCMVmCherry Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FKBP_SCF2_LD6_NTEVMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
FRB_SCF2_LD6_CTEVMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
pFF145Beta recombinase (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF530msd_kanR(ON) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF706SCRIBE_kanR(ON) (Other)Synthetic BiologypDawn Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF714SCRIBE_galK(OFF) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF745SCRIBE_kanR(ON) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF746SCRIBE_galK(ON) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF749msd_kanR(ON)_dRT (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF753msd(wt) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF758msd(wt)_dRT (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF761SCRIBE_galK(OFF) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF828msd_lacZ(ON) (Other)Synthetic BiologypLuxR Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pFF838SCRIBE_lacZ(ON) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
pJKR-H-cdaRbeta lactamase, sfGFP, CdaRBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-cdaRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-mphRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-mphRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-tetRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-tetRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-araCGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-ttgRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-ttgRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-H-acuRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-L-acuRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-O-acuRCFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJKR-O-mphRmCherryBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
pJexpress414_OmpA171_R4consOmpA_R4cons (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_WTOmpA (Other)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1346OmpA_H1346 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1256OmpA_H1256 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2345OmpA_H2345 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H3567OmpA_H3567 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2368OmpA_H2368 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1678OmpA_H1678 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_WTnoGirdlesOmpA_noGirdles (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pSR59.4OmpR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR58.6CcaR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR43.6rCcaS (Other), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pSR33.4rCph8 (Synthetic), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
pJexpress414_OmpA171_H1237OmpA_H1237 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2578OmpA_H2578 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H3478OmpA_H3478 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H2467OmpA_H2467 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1457OmpA_H1457 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H4568OmpA_H4568 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H1358OmpA_H1358 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G125LOmpA_R4cons_G125L (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G41AOmpA_R4cons_G41A (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_G99IOmpA_R4cons_G99I (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_P121FOmpA_R4cons_P121F (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_R4cons_Y129HOmpA_R4cons_Y129H (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-3OmpA_H-3 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-46OmpA_H-46 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H346OmpA_H346 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pJexpress414_OmpA171_H-78OmpA_H-78 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
pSCKbreak12CisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj11TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12-olsaTheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12-o11TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic)Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
pSCKtheoRaj12mpSCKtheoRaj12m (Synthetic)Synthetic Biology ; pSC101 origin of replication Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
Dazl-CPG-TV5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
GAPDH-CPG-TV5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
SOX2se-CPG-TV5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
pWKS1583amyEopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
pAP24sLucopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)sfGFP-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)mCherry-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
pCasper4-g-osk-TRICKoskar (Drosophila melanogaster)Insect Expression ; P element-mediated germline transformationosk6xMS2, 12xPP7 Ephrussi Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
pAS1NB c Rosella IRosella (Synthetic)Yeast ExpressionPGK1 Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
pAS1NB m Rosella IRosella (Synthetic)Yeast ExpressionPGK1yeast mitochondrial targeting sequence of citrate synthase Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
pGM55GM-CSF minimal promoter (-55 to +28) (Homo sapiens)Mammalian Expression, LuciferaseGM-CSF minimalluciferase Cockerill Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Plasmid. 2000 Sep;44(2):173-82.
CY026RhlR, CinRBacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
CY027Bacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
pAWp7UBCp-RFP-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp11LentiviralCMVp Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp12CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-3UBCp-RFP-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-5UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-7UBCp-RFP-[miR-124 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-9UBCp-RFP-[miR-132 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-10UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-12UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-13UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-15UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-16UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-18UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-19UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWp7-20UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pAWSV-22[miR-24-2/27a/23a cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
pTriEx-mCherry-LINXa3LINXa3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionChicken B-ActinmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pTriEx-mCherry-LINXb3LINXb3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pTriEx-mCherry-LINXa3-nanoLINXa3-nano (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pJKR-H-benMmuconate biosensing apparatus Church Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
Flamindo2Flamindo2Mammalian ExpressionCMV Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
nls-Flamindo2nls-Flamindo2Mammalian ExpressionCMV Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
mRFP-Lact-C2Lactadherin-C2 (Bos taurus)Mammalian ExpressionCMVmRFP Grinstein Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11. 319(5860):210-3.
pZ8-P_dCas9dcas9Bacterial Expression, CRISPR, Synthetic BiologyPropionate inducible promoter (prp) Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
pZ8-PrpPropionate inducible promoterBacterial Expression, Synthetic Biologyprp Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
ZIKV_Sensor_27B_LacZZIKV_sensor_27B_LacZ (Synthetic)Bacterial ExpressionT7LacZ Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Sensor_32B_LacZZIKV_sensor_32B_LacZ (Synthetic)Bacterial ExpressionT7LacZ Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Trigger_27BZIKV_Trigger_27B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_Trigger_32BZIKV_Trigger_32B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_NASBA_27BZIKV_NASBA_27B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
ZIKV_NASBA_32BZIKV_NASBA_32B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)Renilla / P19 (Other)Plant Expression, Luciferase, Synthetic Biology35S Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
pET22b_PstS_1pstS (A197C) (Other)Bacterial ExpressionT7 Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
pET22b_PstS_2pstS (A17C,A197C) (Other)Bacterial ExpressionT7 Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
pHis17_ParM _1 parM (K33A I27C T174A T175N C287A) (Other)Bacterial ExpressionunknownHis6 Webb A biosensor for fluorescent determination of ADP with high time resolution. J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29.
pET22b_EcSSB_1ssb (G26C) (Other)Bacterial ExpressionT7 Webb Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity. Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3.
pET22b_EcSSB_2ssb (W88C) (Other)Bacterial ExpressionT7 Webb Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28.
pTEV5_ RpMatB_1matB (His6 C106A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase. ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21.
pTEV5_ RpMatB_2matB (His6 C106A S170A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
pTEV5_ RpMatB_3matB (His6 C106A T167A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
pTEV5_ RpMatB_4matB (His6 C106A R286C T303A Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.

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