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SynBio_Icon_Sensing_and_Signaling.png Synthetic Biology: Sensing and Signaling


Browse Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing.



Sensing and Signaling Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plamsids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Hidden Extra Search Info  
pAL149pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic BiologyCMV Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B Add to Cart
pAL175p!SV40-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2 Add to Cart
pAL188p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2 Add to Cart
pAL189p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2 Add to Cart
pAL190p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2 Add to Cart
pAL197p!SV40-PakGBD-mCherry (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 Add to Cart
pAL198p!SV40-mCherry-WaspGBD (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 Add to Cart
pAL204pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B Add to Cart
pAL113pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B Add to Cart
pAL101pCMV-PIF6APB-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 Add to Cart
pAL130pCMV-PIF3NT-mYFP (Mus musculus)Mammalian Expression, Synthetic Biology Voigt Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():. pcDNA3.1 PIF3 AT1G09530, F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, phytochrome interacting factor 3, purple acid phosphatase 3 Add to Cart
pCph8pCph8Bacterial Expression, Synthetic Biology Voigt Synthetic biology: engineering Escherichia coli to see light. Nature. 2005 Nov 24. 438(7067):441-2. pPROTet Add to Cart
pHL32 (in cHNL135)Bacterial Expression, Synthetic Biology Lim A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75. pHL32 Add to Cart
pJT103PompC-lacZBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81. pSB4A3 Add to Cart
pJT104Edge detector delta luxIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81. pSB4A3 Add to Cart
pJT105Edge detector delta cIBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81. pSB4A3 Add to Cart
pEDL3Edge detectorBacterial Expression, Synthetic Biology Voigt A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81. pSB4A3 Add to Cart
pJT106bred light inverterBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24. BioBrick vector pSB4A3 Add to Cart
pJT106b3red light inverter with lacZ outputBacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24. pSB4A3 Add to Cart
pJT116cca cluster from Synechocystis PCC6803Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24. pProTet.E333 Add to Cart
pJT118pJT118Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24. pProTet.E333 Add to Cart
pJT122pJT122Bacterial Expression, Synthetic Biology Voigt Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24. pProTet.E333 Add to Cart
pET21b-LOV-ipaALOV-ipaA (Synthetic)Bacterial ExpressionT76xHis Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17. pET-21b(+) Add to Cart
pET21b-LOV-ipaA L514K L531ELOV-ipaA L514K L531E (Synthetic)Bacterial ExpressionT76xHis Kuhlman Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17. pET-21b(+)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)
  • Add to Cart
    pZE-PalkB-GFPGFP, PalkBBacterial Expression, Synthetic BiologyPalkB, PalkB Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2. pZE Add to Cart
    pACYC-PLlambda-alkS(WT)AlkS(WT)Bacterial Expression, Synthetic BiologyPLlambda Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2. pACYC Add to Cart
    pACYC-PLlambda-alkSm4-3AlkSm4-3 (Other)Synthetic BiologyPLlambda Alper Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2. pACYC Add to Cart
    pCS-PesaRluxPesaR (Other)Bacterial Expression, Synthetic BiologyPesaR Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. wt esaR promoter in pCS26 pCS26 Add to Cart
    pCS-PluxIluxPluxI (Other)Bacterial Expression, Synthetic BiologyPluxI Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. wt luxI promoter in pCS26 pCS-PesaRlux Add to Cart
    pAC-EsaRPlac-esaR-terminatorBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. wt esaR gene under control of Plac in pACYC184 pACYC184 Add to Cart
    pAC-EsaR-I70V/D91GesaR-I70V/D91GBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a208g and a272g mutations in esaR pAC-EsaR Add to Cart
    pAC-EsaR-V220AesaR-V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a c659t mutation in esaR pAC-EsaR Add to Cart
    pAC-EsaR-D91GesaR-D91GBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a a272g mutation in esaR pAC-EsaR Add to Cart
    pAC-EsaR-I70V/D91G/V220AesaR-I70V/D91G/V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a208g, a272g, c659t mutations in esaR pAC-EsaR Add to Cart
    pAC-EsaR-D91G/V220AesaR-D91G/V220ABacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a272g and c659t mutations in esaR pAC-EsaR Add to Cart
    pCS-PesaR-DluxPesaR-DBacterial Expression, Synthetic BiologyPesaR-D Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR-D promoter in pCS26 pCS-PesaRlux Add to Cart
    pCS-PesaR-PluxPesaR-PBacterial Expression, Synthetic BiologyPesaR-P Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR-P promoter in pCS26 pCS-PesaRlux Add to Cart
    pET-PesaR-DluxPesaR-DBacterial Expression, Synthetic BiologyPesaR-D Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR) pET-PesaRlux Add to Cart
    pET-PesaR-CluxPesaR-CBacterial Expression, Synthetic BiologyPesaR-C Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR) pET-PesaRlux Add to Cart
    pET-PesaR-PluxPesaR-PBacterial Expression, Synthetic BiologyPesaR-P Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR) pET-PesaRlux Add to Cart
    pAC-EsaR-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. esaI gene downstream of esaR in pAC-EsaR (CmR) pAC-EsaR Add to Cart
    pAC-EsaR-I70V-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR) pAC-EsaR-I70V Add to Cart
    pAC-EsaR-D91G-EsaIesaI-termBacterial Expression, Synthetic BiologyPlac Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR) pAC-EsaR-D91G Add to Cart
    pAC-EsaR-I70VesaR-I70VBacterial Expression, Synthetic BiologyPlac Collins Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. pAC-EsaR with a208g mutation in esaR pAC-EsaR Add to Cart
    pET-PesaRluxPesaR-luxCDABE-termBacterial Expression, Synthetic BiologyPesaR Collins Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23. PesaR promoter, luxCDABE, and terminator in pET17b (AmpR) pET-17b Add to Cart
    pTD103aiiAaiiA and LuxRBacterial Expression, Synthetic BiologypLuxssrA (TSAANDENYALAA) Hasty A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753. Negative feedback plasmid from oscillator pZ vector Add to Cart
    pTD103luxI_sfGFPLuxI, sfGFP, and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Positive feedback plasmid from oscillator pZ vector Add to Cart
    pTD103aiiA(Cm)aiiA and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Negative feedback plasmid from oscillator pZ vector Add to Cart
    pTD103luxI_sfGFP -RsfGFP and luxIBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Positive feedback plasmid from oscillator without luxR pZ vector Add to Cart
    pTD103aiiA(Cm) -RaiiABacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Negative feedback plasmid from oscillator without luxR pZ vector Add to Cart
    pZSm45 ndhIIndhII and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Lux-regulated expression of NDH-2 enzyme pZ vector Add to Cart
    pZSm45 sodAsodA LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA) Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. Lux-regulated expression of sodA enzyme pZ vector Add to Cart
    pZSm47a luxR RBSluxRBacterial Expression, Synthetic BiologypArsR Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. ArsR-regulated expression of luxR pZ vector Add to Cart
    pZS47a luxI-laa RBSLuxIBacterial Expression, Synthetic BiologypArsR Hasty A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. ArsR-regulated expression of luxI-laa pZ vector Add to Cart
    pHR SFFVp PHAkt CeruleanPHAktLentiviral, Synthetic BiologyCerulean Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. Fluorescent reporter for membrane PIP3 levels pHR AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA Add to Cart
    pHR SFFVp Phy-mCherry-CAAXPhyB 1-908 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. Optogenetic PI3K activation system; membrane component pHR Add to Cart
    pHR iSH-YFP-PIFp85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana)Lentiviral, Synthetic Biology Lim Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700. Optogenetic PI3K activation system; cytoplasmic component pHR Add to Cart
    pHR SFFVp BFP-Erk2Erk2 (Mus musculus)LentiviralTagBFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. Fluorescent reporter for Erk nuclear translocation pHR Mapk1 9030612K14Rik, AA407128, AU018647, C78273, ERK, Erk2, MAPK2, PRKM2, Prkm1, p41mapk, p42mapk Add to Cart
    pHR SFFVp BFP-PIF-SOS2catSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)LentiviralTagBFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. Optogenetic Ras activation system; cytoplasmic component pHR SOS2 NS9 Add to Cart
    pHR SFFVp YFP-PIF-SOS2catSOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)LentiviralYFP Lim Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004. Optogenetic Ras activation system; cytoplasmic component pHR SOS2 NS9 Add to Cart
    pP3-GFPP3 promoter (Other)Bacterial Expression, Synthetic BiologyP3none Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome pPT7-GFP Add to Cart
    pXylA-agrCA-IagrCA locus (Other)Bacterial Expression, Synthetic BiologyPxylAnone Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. xylose-dependent expression of the type-I agrC and agrA genes of S. aureus pT7-RNAP Add to Cart
    pXylA-agrCA-IVagrCA locus (Other)Bacterial Expression, Synthetic BiologyPxylAnone Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus pT7-RNAP Add to Cart
    pT7-agrBD-IagrBD locus (Other)Bacterial Expression, Synthetic BiologyT7-lacV5 epitope, 6x HIS (not expressed on agrB or agrD) Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220 pET101 D-TOPO Add to Cart
    pT7-agrBD-IVagrBD locus (with D29Y mutation in agrD) (Other)Bacterial Expression, Synthetic BiologyT7-lacV5 epitope, 6x HIS (not expressed on agrB or agrD) Collins Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV pET101 D-TOPO Add to Cart
    tTA_BFPtTA transcription factor fused to enhanced blue fluorescent protein (Synthetic)Mammalian ExpressionCMVBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901) pcDNA3.1 Add to Cart
    pBI-MCS-EYFPenhanced yellow fluorescent protein (Synthetic)Mammalian ExpressionCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP pBI-MCS-EGFP Add to Cart
    mCherry_LD 0_CS G_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    mCherry_LD 0_TEVMESA protease chain with mCherry ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA protease chain with mCherry ectodomain and no linker domain pAAV Add to Cart
    CD4_LD 0_CS G_tTA-BFPMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    CD4_LD 0_TEVMESA protease chain with CD4 ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA protease chain with CD4 ectodomain and no linker domain pAAV Add to Cart
    dTomato_LD 0_CS G_tTA-BFPMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    dTomato_LD 0_TEVMESA protease chain with dTomato ectodomain and TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA protease chain with dTomato ectodomain and no linker domain pAAV Add to Cart
    mCherry_LD 0_CS A_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    mCherry_LD 0_CS M_tTA-BFPMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    dTomato_LD 0_CS M_tTA-BFPMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    FRB_LD 0_CS G_tTA-BFPMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)Mammalian Expression, AAVCMVEBFP Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion pAAV Add to Cart
    FKBP_LD 0_TEVMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic)Mammalian Expression, AAVCMVmCherry Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA protease chain with Rapamycin FKBP ectodomain and no linker domain pAAV Add to Cart
    FKBP_SCF2_LD6_NTEVMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease pAAV Add to Cart
    FRB_SCF2_LD6_CTEVMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic)Mammalian Expression, AAVCMV Leonard Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11. MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease pAAV Add to Cart
    pFF145Beta recombinase (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. aTc-inducible Beta recombinase pZA11 Add to Cart
    pFF530msd_kanR(ON) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible msd_kanR(ON) pZE32 Add to Cart
    pFF706SCRIBE_kanR(ON) (Other)Synthetic BiologypDawn Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. Light-inducible SCRIBE_kanR(ON) pZE32 Add to Cart
    pFF714SCRIBE_galK(OFF) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible SCRIBE_galK(OFF) pZE32 Add to Cart
    pFF745SCRIBE_kanR(ON) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible SCRIBE_kanR(ON) pZE32 Add to Cart
    pFF746SCRIBE_galK(ON) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. aTc-inducible SCRIBE_galK(ON) pZA21 Add to Cart
    pFF749msd_kanR(ON)_dRT (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible msd_kanR(ON)_dRT pZE32 Add to Cart
    pFF753msd(wt) (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible msd(wt) pZE32 Add to Cart
    pFF758msd(wt)_dRT (Other)Synthetic BiologypLacO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. IPTG-inducible msd(wt)_dRT pZE32 Add to Cart
    pFF761SCRIBE_galK(OFF) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. aTc-inducible SCRIBE_galK(OFF) pZA11 Add to Cart
    pFF828msd_lacZ(ON) (Other)Synthetic BiologypLuxR Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. AHL-inducible msd_lacZ(ON) pZE32 Add to Cart
    pFF838SCRIBE_lacZ(ON) (Other)Synthetic BiologypTetO Lu Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272. aTc-inducible SCRIBE_lacZ(ON) pZA31 Add to Cart
    pJKR-H-cdaRbeta lactamase, sfGFP, CdaRBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration. pUC Add to Cart
    pJKR-L-cdaRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration. SC101 Add to Cart
    pJKR-H-mphRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration. pUC Add to Cart
    pJKR-L-mphRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration. SC101 Add to Cart
    pJKR-H-tetRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration. pUC Add to Cart
    pJKR-L-tetRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration. SC101 Add to Cart
    pJKR-H-araCGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration. pUC Add to Cart
    pJKR-H-ttgRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration. pUC Add to Cart
    pJKR-L-ttgRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration. SC101 Add to Cart
    pJKR-H-acuRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration. pUC Add to Cart
    pJKR-L-acuRGFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration. SC101 Add to Cart
    pJKR-O-acuRCFPBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids. colA Add to Cart
    pJKR-O-mphRmCherryBacterial Expression Church Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids. p15a Add to Cart
    pJexpress414_OmpA171_R4consOmpA_R4cons (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 pJexpress Add to Cart
    pJexpress414_OmpA171_WTOmpA (Other)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop pJexpress414 Add to Cart
    pJexpress414_OmpA171_H1346OmpA_H1346 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1346 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H1256OmpA_H1256 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1256 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H2345OmpA_H2345 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2345 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H3567OmpA_H3567 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 3567 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H2368OmpA_H2368 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2368 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H1678OmpA_H1678 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1678 pJexpress414 Add to Cart
    pJexpress414_OmpA171_WTnoGirdlesOmpA_noGirdles (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Wild-type OmpA with aromatic girdle residues replaced by leucine pJexpress414 Add to Cart
    pSR59.4OmpR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter pSB4A3 ompR b3405, ECK3392, cry, kmt, ompB Add to Cart
    pSR58.6CcaR (Other), sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter pProTet.E333 Add to Cart
    pSR43.6rCcaS (Other), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. Expresses CcaS, Ho1, and PcyA constitutively pACYCDuet-1 Add to Cart
    pSR33.4rCph8 (Synthetic), Ho1 (Other), PcyA (Other)Bacterial Expression, Synthetic Biology Tabor Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. Expresses Cph8, Ho1, and PcyA constitutively pACYCDuet-1 Add to Cart
    pJexpress414_OmpA171_H1237OmpA_H1237 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1237 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H2578OmpA_H2578 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2578 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H3478OmpA_H3478 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 3478 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H2467OmpA_H2467 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2467 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H1457OmpA_H1457 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1457 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H4568OmpA_H4568 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 4568 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H1358OmpA_H1358 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1358 pJexpress414 Add to Cart
    pJexpress414_OmpA171_R4cons_G125LOmpA_R4cons_G125L (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G125L mutation pJexpress Add to Cart
    pJexpress414_OmpA171_R4cons_G41AOmpA_R4cons_G41A (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G41A mutation pJexpress Add to Cart
    pJexpress414_OmpA171_R4cons_G99IOmpA_R4cons_G99I (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G99I mutation pJexpress Add to Cart
    pJexpress414_OmpA171_R4cons_P121FOmpA_R4cons_P121F (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with P121F mutation pJexpress Add to Cart
    pJexpress414_OmpA171_R4cons_Y129HOmpA_R4cons_Y129H (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with Y129H mutation pJexpress Add to Cart
    pJexpress414_OmpA171_H-3OmpA_H-3 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1245678 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H-46OmpA_H-46 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 123578 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H346OmpA_H346 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 346 pJexpress414 Add to Cart
    pJexpress414_OmpA171_H-78OmpA_H-78 (Synthetic)Bacterial Expression, Synthetic BiologyT76His, FlAsH Whitehead Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 123456 pJexpress414 Add to Cart
    pSCKbreak12CisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC2,cisRaj12-sfGFP, KanR pSTC2 Add to Cart
    pSCKtheoRaj11TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR pSTC1 Add to Cart
    pSCKtheoRaj12-olsaTheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR pSTC2 Add to Cart
    pSCKtheoRaj12-o11TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic)Bacterial Expression, Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR pSTC2 Add to Cart
    pSCKtheoRaj12TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic)Synthetic Biology Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR pSTC2 Add to Cart
    pSCKtheoRaj12mpSCKtheoRaj12m (Synthetic)Synthetic Biology ; pSC101 origin of replication Jaramillo Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27. pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR pSTC2 Add to Cart
    Dazl-CPG-TV5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA topo Add to Cart
    GAPDH-CPG-TV5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA topo Add to Cart
    SOX2se-CPG-TV5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic)Mouse Targeting, Synthetic Biology Jaenisch Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA topo Add to Cart
    pWKS1583amyEopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23. Modular vector for secreted amylase reporter expression pRPF185 Add to Cart
    pAP24sLucopt (Synthetic)Bacterial Expression, Synthetic BiologyPtet Smits The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23. Modular vector for secreted luciferase reporter expression pRPF185 Add to Cart
    pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)sfGFP-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. 1 color weak reporter used in study n/a Add to Cart
    pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)mCherry-ssrA Murray Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. for building 3-color reporter plasmid n/a Add to Cart
    pCasper4-g-osk-TRICKoskar (Drosophila melanogaster)Insect Expression ; P element-mediated germline transformationosk12xPP7, 6xMS2 Ephrussi Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila. pCasper4 osk Dmel_CG10901, CG10901, Dm-osk, Dmel\CG10901, OSK, Osk, Oskar, norka Add to Cart
    pAS1NB c Rosella IRosella (Synthetic)Yeast ExpressionPGK1 Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21. a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization. pAS1NB Add to Cart
    pAS1NB m Rosella IRosella (Synthetic)Yeast ExpressionPGK1yeast mitochondrial targeting sequence of citrate synthase Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21. a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization. pAS1NB Add to Cart
    pGM55GM-CSF minimal promoter (-55 to +28) (Homo sapiens)Mammalian Expression, LuciferaseGM-CSF minimalluciferase Cockerill Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Plasmid. 2000 Sep;44(2):173-82. luciferase reporter with hGM-CSF -55 to +28 minimal promoter pXPG CSF2 CSF, GMCSF Add to Cart
    CY026RhlR, CinRBacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794. Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR E. coli BW25113 rhlR PA3477 Add to Cart
    CY027Bacterial Expression, Synthetic Biology Bennett SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794. Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR E. coli BW25113 Add to Cart
    pAWp7UBCp-RFP-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-CMVp-GFP pFUGW Add to Cart
    pAWp11LentiviralCMVp Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-CMVp pFUGW Add to Cart
    pAWp12CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-CMVp-GFP pFUGW Add to Cart
    pAWp7-3UBCp-RFP-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-CMVp-GFP-[miR-132] pFUGW Add to Cart
    pAWp7-5UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132] pFUGW Add to Cart
    pAWp7-7UBCp-RFP-[miR-124 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP pFUGW Add to Cart
    pAWp7-9UBCp-RFP-[miR-132 sensor]-CMVp-GFPLentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP pFUGW Add to Cart
    pAWp7-10UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124] pFUGW Add to Cart
    pAWp7-12UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124] pFUGW Add to Cart
    pAWp7-13UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128] pFUGW Add to Cart
    pAWp7-15UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128] pFUGW Add to Cart
    pAWp7-16UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132] pFUGW Add to Cart
    pAWp7-18UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132] pFUGW Add to Cart
    pAWp7-19UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128] pFUGW Add to Cart
    pAWp7-20UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132] pFUGW Add to Cart
    pAWSV-22[miR-24-2/27a/23a cluster] Lu Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17. pBT264-[miR-24-2/27a/23a cluster] pBT264 Add to Cart
    pLux76-LasILasI (Other)Synthetic BiologypLux76 Haseloff Orthogonal intercellular signaling for programmed spatial behavior. Mol Syst Biol. 2016 Jan 25;12(1):849. HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL pSB6A1 Add to Cart
    pLas81-LuxILuxI (Other)Synthetic BiologypLas81 Haseloff Orthogonal intercellular signaling for programmed spatial behavior. Mol Syst Biol. 2016 Jan 25;12(1):849. HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL pSB6A1 Add to Cart
    pTriEx-mCherry-LINXa3LINXa3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionChicken B-ActinmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18. Light-induced nuclear export switch for microscopy in mammalian cells. pTriEx Add to Cart
    pTriEx-mCherry-LINXb3LINXb3 (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18. Light-induced nuclear export switch for microscopy in mammalian cells. pTriEx Add to Cart
    pTriEx-mCherry-LINXa3-nanoLINXa3-nano (Synthetic)Mammalian Expression, Bacterial Expression, Insect ExpressionmCherry Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18. Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells. pTriEx Add to Cart
    pJKR-H-benMmuconate biosensing apparatus Church Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375. Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell. pUC Add to Cart
    Flamindo2Flamindo2Mammalian ExpressionCMV Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014. Expresses biosensor for cAMP in mammalian cells pcDNA3.1 (-) Add to Cart
    nls-Flamindo2nls-Flamindo2Mammalian ExpressionCMV Kitaguchi Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014. Expresses biosensor for cAMP in mammalian cells with nuclear localization signal pcDNA3.1 (-) Add to Cart
    mRFP-Lact-C2Lactadherin-C2 (Bos taurus)Mammalian ExpressionCMVmRFP Grinstein Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11. 319(5860):210-3. Fluorescent biosensor for phosphatidylserine. mRFP-C1 MFGE8 Add to Cart
    pZ8-P_dCas9dcas9Bacterial Expression, CRISPR, Synthetic BiologyPropionate inducible promoter (prp) Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR pZ8-Prp Add to Cart
    pZ8-PrpPropionate inducible promoterBacterial Expression, Synthetic Biologyprp Lu Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16. pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR pZ8-1 Add to Cart
    ZIKV_Sensor_27B_LacZZIKV_sensor_27B_LacZ (Synthetic)Bacterial ExpressionT7LacZ Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Toehold switch sensor to detect Zika Virus pCOLA Duet
  • Tag / Fusion Protein
    • LacZ (C terminal on insert)
  • Add to Cart
    ZIKV_Sensor_32B_LacZZIKV_sensor_32B_LacZ (Synthetic)Bacterial ExpressionT7LacZ Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Toehold switch sensor to detect Zika Virus pCOLA Duet
  • Tag / Fusion Protein
    • LacZ (C terminal on insert)
  • Add to Cart
    ZIKV_Trigger_27BZIKV_Trigger_27B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence pET-15b Add to Cart
    ZIKV_Trigger_32BZIKV_Trigger_32B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence pET-15b Add to Cart
    ZIKV_NASBA_27BZIKV_NASBA_27B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites pET-15b Add to Cart
    ZIKV_NASBA_32BZIKV_NASBA_32B (Synthetic)Bacterial ExpressionT7 Collins Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059. Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites pET-15b Add to Cart
    pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)Renilla / P19 (Other)Plant Expression, Luciferase, Synthetic Biology35S Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. Module for the expression of the Renilla Luciferase with the silencing suppressor P19 pDGB1alpha2 Add to Cart
    pET22b_PstS_1pstS (A197C) (Other)Bacterial ExpressionT7 Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4. Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor pET22b Add to Cart
    pET22b_PstS_2pstS (A17C,A197C) (Other)Bacterial ExpressionT7 Webb Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4. Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor pET22b Add to Cart
    pHis17_ParM _1 parM (K33A I27C T174A T175N C287A) (Other)Bacterial ExpressionunknownHis6 Webb A biosensor for fluorescent determination of ADP with high time resolution. J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29. Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor pHis17
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)
  • Add to Cart
    pET22b_EcSSB_1ssb (G26C) (Other)Bacterial ExpressionT7 Webb Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity. Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3. Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor pET22b Add to Cart
    pET22b_EcSSB_2ssb (W88C) (Other)Bacterial ExpressionT7 Webb Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28. Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor pET22b Add to Cart
    pTEV5_ RpMatB_1matB (His6 C106A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase. ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21. Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor pTEV5
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
  • Add to Cart
    pTEV5_ RpMatB_2matB (His6 C106A S170A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding pTEV5
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
  • Add to Cart
    pTEV5_ RpMatB_3matB (His6 C106A T167A R286C Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding pTEV5
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
  • Add to Cart
    pTEV5_ RpMatB_4matB (His6 C106A R286C T303A Q457C K488A) (Other)Bacterial ExpressionT7His6 Webb Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding pTEV5
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)
  • Add to Cart

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