| pAL149 | pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | CMV | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
|
Add to Cart
|
| pAL175 | p!SV40-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2
|
Add to Cart
|
| pAL188 | p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2
|
Add to Cart
|
| pAL189 | p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2
|
Add to Cart
|
| pAL190 | p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PIL2 AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2
|
Add to Cart
|
| pAL197 | p!SV40-PakGBD-mCherry (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
|
Add to Cart
|
| pAL198 | p!SV40-mCherry-WaspGBD (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
|
Add to Cart
|
| pAL204 | pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
|
Add to Cart
|
| pAL113 | pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PHYB AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
|
Add to Cart
|
| pAL101 | pCMV-PIF6APB-mYFP (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
|
Add to Cart
|
| pAL130 | pCMV-PIF3NT-mYFP (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
pcDNA3.1
PIF3 AT1G09530, F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, phytochrome interacting factor 3, purple acid phosphatase 3
|
Add to Cart
|
| pCph8 | pCph8 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Synthetic biology: engineering Escherichia coli to see light.
Nature. 2005 Nov 24. 438(7067):441-2.
|
pPROTet
|
Add to Cart
|
| pHL32 (in cHNL135) | | Bacterial Expression, Synthetic Biology | | |
Lim
| A multistep epigenetic switch enables the stable inheritance of DNA methylation states.
Nat Genet. 2007 Feb . 39(2):269-75.
|
pHL32
|
Add to Cart
|
| pJT103 | PompC-lacZ | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
pSB4A3
|
Add to Cart
|
| pJT104 | Edge detector delta luxI | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
pSB4A3
|
Add to Cart
|
| pJT105 | Edge detector delta cI | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
pSB4A3
|
Add to Cart
|
| pEDL3 | Edge detector | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
pSB4A3
|
Add to Cart
|
| pJT106b | red light inverter | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
BioBrick vector pSB4A3
|
Add to Cart
|
| pJT106b3 | red light inverter with lacZ output | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
pSB4A3
|
Add to Cart
|
| pJT116 | cca cluster from Synechocystis PCC6803 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
pProTet.E333
|
Add to Cart
|
| pJT118 | pJT118 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
pProTet.E333
|
Add to Cart
|
| pJT122 | pJT122 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
pProTet.E333
|
Add to Cart
|
| pET21b-LOV-ipaA | LOV-ipaA (Synthetic) | Bacterial Expression | T7 | 6xHis |
Kuhlman
| Designing photoswitchable peptides using the AsLOV2 domain.
Chem Biol. 2012 Apr 20;19(4):507-17.
|
pET-21b(+)
|
Add to Cart
|
| pET21b-LOV-ipaA L514K L531E | LOV-ipaA L514K L531E (Synthetic) | Bacterial Expression | T7 | 6xHis |
Kuhlman
| Designing photoswitchable peptides using the AsLOV2 domain.
Chem Biol. 2012 Apr 20;19(4):507-17.
|
pET-21b(+)
Tag
/ Fusion Protein- 6xHis (N terminal on insert)
|
Add to Cart
|
| pZE-PalkB-GFP | GFP, PalkB | Bacterial Expression, Synthetic Biology | PalkB, PalkB | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
pZE
|
Add to Cart
|
| pACYC-PLlambda-alkS(WT) | AlkS(WT) | Bacterial Expression, Synthetic Biology | PLlambda | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
pACYC
|
Add to Cart
|
| pACYC-PLlambda-alkSm4-3 | AlkSm4-3 (Other) | Synthetic Biology | PLlambda | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
pACYC
|
Add to Cart
|
| pCS-PesaRlux | PesaR (Other) | Bacterial Expression, Synthetic Biology | PesaR | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
wt esaR promoter in pCS26
pCS26
|
Add to Cart
|
| pCS-PluxIlux | PluxI (Other) | Bacterial Expression, Synthetic Biology | PluxI | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
wt luxI promoter in pCS26
pCS-PesaRlux
|
Add to Cart
|
| pAC-EsaR | Plac-esaR-terminator | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
wt esaR gene under control of Plac in pACYC184
pACYC184
|
Add to Cart
|
| pAC-EsaR-I70V/D91G | esaR-I70V/D91G | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a208g and a272g mutations in esaR
pAC-EsaR
|
Add to Cart
|
| pAC-EsaR-V220A | esaR-V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a c659t mutation in esaR
pAC-EsaR
|
Add to Cart
|
| pAC-EsaR-D91G | esaR-D91G | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a a272g mutation in esaR
pAC-EsaR
|
Add to Cart
|
| pAC-EsaR-I70V/D91G/V220A | esaR-I70V/D91G/V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a208g, a272g, c659t mutations in esaR
pAC-EsaR
|
Add to Cart
|
| pAC-EsaR-D91G/V220A | esaR-D91G/V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a272g and c659t mutations in esaR
pAC-EsaR
|
Add to Cart
|
| pCS-PesaR-Dlux | PesaR-D | Bacterial Expression, Synthetic Biology | PesaR-D | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR-D promoter in pCS26
pCS-PesaRlux
|
Add to Cart
|
| pCS-PesaR-Plux | PesaR-P | Bacterial Expression, Synthetic Biology | PesaR-P | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR-P promoter in pCS26
pCS-PesaRlux
|
Add to Cart
|
| pET-PesaR-Dlux | PesaR-D | Bacterial Expression, Synthetic Biology | PesaR-D | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)
pET-PesaRlux
|
Add to Cart
|
| pET-PesaR-Clux | PesaR-C | Bacterial Expression, Synthetic Biology | PesaR-C | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)
pET-PesaRlux
|
Add to Cart
|
| pET-PesaR-Plux | PesaR-P | Bacterial Expression, Synthetic Biology | PesaR-P | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)
pET-PesaRlux
|
Add to Cart
|
| pAC-EsaR-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
esaI gene downstream of esaR in pAC-EsaR (CmR)
pAC-EsaR
|
Add to Cart
|
| pAC-EsaR-I70V-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR)
pAC-EsaR-I70V
|
Add to Cart
|
| pAC-EsaR-D91G-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)
pAC-EsaR-D91G
|
Add to Cart
|
| pAC-EsaR-I70V | esaR-I70V | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
pAC-EsaR with a208g mutation in esaR
pAC-EsaR
|
Add to Cart
|
| pET-PesaRlux | PesaR-luxCDABE-term | Bacterial Expression, Synthetic Biology | PesaR | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
PesaR promoter, luxCDABE, and terminator in pET17b (AmpR)
pET-17b
|
Add to Cart
|
| pTD103aiiA | aiiA and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA (TSAANDENYALAA) |
Hasty
| A synchronized quorum of genetic clocks.
Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753.
|
Negative feedback plasmid from oscillator
pZ vector
|
Add to Cart
|
| pTD103luxI_sfGFP | LuxI, sfGFP, and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Positive feedback plasmid from oscillator
pZ vector
|
Add to Cart
|
| pTD103aiiA(Cm) | aiiA and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Negative feedback plasmid from oscillator
pZ vector
|
Add to Cart
|
| pTD103luxI_sfGFP -R | sfGFP and luxI | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Positive feedback plasmid from oscillator without luxR
pZ vector
|
Add to Cart
|
| pTD103aiiA(Cm) -R | aiiA | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Negative feedback plasmid from oscillator without luxR
pZ vector
|
Add to Cart
|
| pZSm45 ndhII | ndhII and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Lux-regulated expression of NDH-2 enzyme
pZ vector
|
Add to Cart
|
| pZSm45 sodA | sodA LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
Lux-regulated expression of sodA enzyme
pZ vector
|
Add to Cart
|
| pZSm47a luxR RBS | luxR | Bacterial Expression, Synthetic Biology | pArsR | |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
ArsR-regulated expression of luxR
pZ vector
|
Add to Cart
|
| pZS47a luxI-laa RBS | LuxI | Bacterial Expression, Synthetic Biology | pArsR | |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
ArsR-regulated expression of luxI-laa
pZ vector
|
Add to Cart
|
| pHR SFFVp PHAkt Cerulean | PHAkt | Lentiviral, Synthetic Biology | | Cerulean |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
Fluorescent reporter for membrane PIP3 levels
pHR
AKT1 AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
|
Add to Cart
|
| pHR SFFVp Phy-mCherry-CAAX | PhyB 1-908 (Arabidopsis thaliana) | Lentiviral, Synthetic Biology | | |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
Optogenetic PI3K activation system; membrane component
pHR
|
Add to Cart
|
| pHR iSH-YFP-PIF | p85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral, Synthetic Biology | | |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
Optogenetic PI3K activation system; cytoplasmic component
pHR
|
Add to Cart
|
| pHR SFFVp BFP-Erk2 | Erk2 (Mus musculus) | Lentiviral | | TagBFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
Fluorescent reporter for Erk nuclear translocation
pHR
Mapk1 9030612K14Rik, AA407128, AU018647, C78273, ERK, Erk2, MAPK2, PRKM2, Prkm1, p41mapk, p42mapk
|
Add to Cart
|
| pHR SFFVp BFP-PIF-SOS2cat | SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral | | TagBFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
Optogenetic Ras activation system; cytoplasmic component
pHR
SOS2 NS9
|
Add to Cart
|
| pHR SFFVp YFP-PIF-SOS2cat | SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral | | YFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
Optogenetic Ras activation system; cytoplasmic component
pHR
SOS2 NS9
|
Add to Cart
|
| pP3-GFP | P3 promoter (Other) | Bacterial Expression, Synthetic Biology | P3 | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome
pPT7-GFP
|
Add to Cart
|
| pXylA-agrCA-I | agrCA locus (Other) | Bacterial Expression, Synthetic Biology | PxylA | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
xylose-dependent expression of the type-I agrC and agrA genes of S. aureus
pT7-RNAP
|
Add to Cart
|
| pXylA-agrCA-IV | agrCA locus (Other) | Bacterial Expression, Synthetic Biology | PxylA | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus
pT7-RNAP
|
Add to Cart
|
| pT7-agrBD-I | agrBD locus (Other) | Bacterial Expression, Synthetic Biology | T7-lac | V5 epitope, 6x HIS (not expressed on agrB or agrD) |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
pET101 D-TOPO
|
Add to Cart
|
| pT7-agrBD-IV | agrBD locus (with D29Y mutation in agrD) (Other) | Bacterial Expression, Synthetic Biology | T7-lac | V5 epitope, 6x HIS (not expressed on agrB or agrD) |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
pET101 D-TOPO
|
Add to Cart
|
| tTA_BFP | tTA transcription factor fused to enhanced blue fluorescent protein (Synthetic) | Mammalian Expression | CMV | BFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)
pcDNA3.1
|
Add to Cart
|
| pBI-MCS-EYFP | enhanced yellow fluorescent protein (Synthetic) | Mammalian Expression | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
pBI-MCS-EGFP
|
Add to Cart
|
| mCherry_LD 0_CS G_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| mCherry_LD 0_TEV | MESA protease chain with mCherry ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA protease chain with mCherry ectodomain and no linker domain
pAAV
|
Add to Cart
|
| CD4_LD 0_CS G_tTA-BFP | MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| CD4_LD 0_TEV | MESA protease chain with CD4 ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA protease chain with CD4 ectodomain and no linker domain
pAAV
|
Add to Cart
|
| dTomato_LD 0_CS G_tTA-BFP | MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| dTomato_LD 0_TEV | MESA protease chain with dTomato ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA protease chain with dTomato ectodomain and no linker domain
pAAV
|
Add to Cart
|
| mCherry_LD 0_CS A_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| mCherry_LD 0_CS M_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| dTomato_LD 0_CS M_tTA-BFP | MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| FRB_LD 0_CS G_tTA-BFP | MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
pAAV
|
Add to Cart
|
| FKBP_LD 0_TEV | MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic) | Mammalian Expression, AAV | CMV | mCherry |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA protease chain with Rapamycin FKBP ectodomain and no linker domain
pAAV
|
Add to Cart
|
| FKBP_SCF2_LD6_NTEV | MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
pAAV
|
Add to Cart
|
| FRB_SCF2_LD6_CTEV | MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
pAAV
|
Add to Cart
|
| pFF145 | Beta recombinase (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
aTc-inducible Beta recombinase
pZA11
|
Add to Cart
|
| pFF530 | msd_kanR(ON) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible msd_kanR(ON)
pZE32
|
Add to Cart
|
| pFF706 | SCRIBE_kanR(ON) (Other) | Synthetic Biology | pDawn | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
Light-inducible SCRIBE_kanR(ON)
pZE32
|
Add to Cart
|
| pFF714 | SCRIBE_galK(OFF) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible SCRIBE_galK(OFF)
pZE32
|
Add to Cart
|
| pFF745 | SCRIBE_kanR(ON) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible SCRIBE_kanR(ON)
pZE32
|
Add to Cart
|
| pFF746 | SCRIBE_galK(ON) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
aTc-inducible SCRIBE_galK(ON)
pZA21
|
Add to Cart
|
| pFF749 | msd_kanR(ON)_dRT (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible msd_kanR(ON)_dRT
pZE32
|
Add to Cart
|
| pFF753 | msd(wt) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible msd(wt)
pZE32
|
Add to Cart
|
| pFF758 | msd(wt)_dRT (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
IPTG-inducible msd(wt)_dRT
pZE32
|
Add to Cart
|
| pFF761 | SCRIBE_galK(OFF) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
aTc-inducible SCRIBE_galK(OFF)
pZA11
|
Add to Cart
|
| pFF828 | msd_lacZ(ON) (Other) | Synthetic Biology | pLuxR | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
AHL-inducible msd_lacZ(ON)
pZE32
|
Add to Cart
|
| pFF838 | SCRIBE_lacZ(ON) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
aTc-inducible SCRIBE_lacZ(ON)
pZA31
|
Add to Cart
|
| pJKR-H-cdaR | beta lactamase, sfGFP, CdaR | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.
pUC
|
Add to Cart
|
| pJKR-L-cdaR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.
SC101
|
Add to Cart
|
| pJKR-H-mphR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.
pUC
|
Add to Cart
|
| pJKR-L-mphR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.
SC101
|
Add to Cart
|
| pJKR-H-tetR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.
pUC
|
Add to Cart
|
| pJKR-L-tetR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.
SC101
|
Add to Cart
|
| pJKR-H-araC | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.
pUC
|
Add to Cart
|
| pJKR-H-ttgR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.
pUC
|
Add to Cart
|
| pJKR-L-ttgR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.
SC101
|
Add to Cart
|
| pJKR-H-acuR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.
pUC
|
Add to Cart
|
| pJKR-L-acuR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.
SC101
|
Add to Cart
|
| pJKR-O-acuR | CFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.
colA
|
Add to Cart
|
| pJKR-O-mphR | mCherry | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.
p15a
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons | OmpA_R4cons (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_WT | OmpA (Other) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H1346 | OmpA_H1346 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1346
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H1256 | OmpA_H1256 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1256
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H2345 | OmpA_H2345 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 2345
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H3567 | OmpA_H3567 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 3567
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H2368 | OmpA_H2368 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 2368
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H1678 | OmpA_H1678 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1678
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_WTnoGirdles | OmpA_noGirdles (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Wild-type OmpA with aromatic girdle residues replaced by leucine
pJexpress414
|
Add to Cart
|
| pSR59.4 | OmpR (Other), sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter
pSB4A3
ompR b3405, ECK3392, cry, kmt, ompB
|
Add to Cart
|
| pSR58.6 | CcaR (Other), sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter
pProTet.E333
|
Add to Cart
|
| pSR43.6r | CcaS (Other), Ho1 (Other), PcyA (Other) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
Expresses CcaS, Ho1, and PcyA constitutively
pACYCDuet-1
|
Add to Cart
|
| pSR33.4r | Cph8 (Synthetic), Ho1 (Other), PcyA (Other) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
Expresses Cph8, Ho1, and PcyA constitutively
pACYCDuet-1
|
Add to Cart
|
| pJexpress414_OmpA171_H1237 | OmpA_H1237 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1237
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H2578 | OmpA_H2578 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 2578
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H3478 | OmpA_H3478 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 3478
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H2467 | OmpA_H2467 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 2467
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H1457 | OmpA_H1457 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1457
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H4568 | OmpA_H4568 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 4568
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H1358 | OmpA_H1358 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1358
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons_G125L | OmpA_R4cons_G125L (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4 with G125L mutation
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons_G41A | OmpA_R4cons_G41A (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4 with G41A mutation
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons_G99I | OmpA_R4cons_G99I (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4 with G99I mutation
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons_P121F | OmpA_R4cons_P121F (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4 with P121F mutation
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_R4cons_Y129H | OmpA_R4cons_Y129H (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
Conservative version of OmpA Redesign 4 with Y129H mutation
pJexpress
|
Add to Cart
|
| pJexpress414_OmpA171_H-3 | OmpA_H-3 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 1245678
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H-46 | OmpA_H-46 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 123578
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H346 | OmpA_H346 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 346
pJexpress414
|
Add to Cart
|
| pJexpress414_OmpA171_H-78 | OmpA_H-78 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | 6His, FlAsH |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
OmpA redesign hybrid with wild-type strands 123456
pJexpress414
|
Add to Cart
|
| pSCKbreak12 | CisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,cisRaj12-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj11 | TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR
pSTC1
|
Add to Cart
|
| pSCKtheoRaj12-olsa | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj12-o11 | TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj12 | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic) | Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR
pSTC2
|
Add to Cart
|
| pSCKtheoRaj12m | pSCKtheoRaj12m (Synthetic) | Synthetic Biology ; pSC101 origin of replication | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR
pSTC2
|
Add to Cart
|
| Dazl-CPG-TV | 5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA
topo
|
Add to Cart
|
| GAPDH-CPG-TV | 5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA
topo
|
Add to Cart
|
| SOX2se-CPG-TV | 5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA
topo
|
Add to Cart
|
| pWKS1583 | amyEopt (Synthetic) | Bacterial Expression, Synthetic Biology | Ptet | |
Smits
| The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synth Biol. 2016 Jun 23.
|
Modular vector for secreted amylase reporter expression
pRPF185
|
Add to Cart
|
| pAP24 | sLucopt (Synthetic) | Bacterial Expression, Synthetic Biology | Ptet | |
Smits
| The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synth Biol. 2016 Jun 23.
|
Modular vector for secreted luciferase reporter expression
pRPF185
|
Add to Cart
|
| pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632) | sfGFP-ssrA | | | |
Murray
| Rapid cell-free forward engineering of novel genetic ring oscillators.
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
|
1 color weak reporter used in study
n/a
|
Add to Cart
|
| pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588) | mCherry-ssrA | | | |
Murray
| Rapid cell-free forward engineering of novel genetic ring oscillators.
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
|
for building 3-color reporter plasmid
n/a
|
Add to Cart
|
| pCasper4-g-osk-TRICK | oskar (Drosophila melanogaster) | Insect Expression ; P element-mediated germline transformation | osk | 12xPP7, 6xMS2 |
Ephrussi
| Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.
Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
|
oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
pCasper4
osk Dmel_CG10901, CG10901, Dm-osk, Dmel\CG10901, OSK, Osk, Oskar, norka
|
Add to Cart
|
| pAS1NB c Rosella I | Rosella (Synthetic) | Yeast Expression | PGK1 | |
Prescott
| Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast.
Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
|
a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.
pAS1NB
|
Add to Cart
|
| pAS1NB m Rosella I | Rosella (Synthetic) | Yeast Expression | PGK1 | yeast mitochondrial targeting sequence of citrate synthase |
Prescott
| Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast.
Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
|
a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.
pAS1NB
|
Add to Cart
|
| pGM55 | GM-CSF minimal promoter (-55 to +28) (Homo sapiens) | Mammalian Expression, Luciferase | GM-CSF minimal | luciferase |
Cockerill
| Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication.
Plasmid. 2000 Sep;44(2):173-82.
|
luciferase reporter with hGM-CSF -55 to +28 minimal promoter
pXPG
CSF2 CSF, GMCSF
|
Add to Cart
|
| CY026 | RhlR, CinR | Bacterial Expression, Synthetic Biology | | |
Bennett
| SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium.
Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
|
Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR
E. coli BW25113
rhlR PA3477
|
Add to Cart
|
| CY027 | | Bacterial Expression, Synthetic Biology | | |
Bennett
| SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium.
Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
|
Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR
E. coli BW25113
|
Add to Cart
|
| pAWp7 | UBCp-RFP-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-CMVp-GFP
pFUGW
|
Add to Cart
|
| pAWp11 | | Lentiviral | CMVp | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-CMVp
pFUGW
|
Add to Cart
|
| pAWp12 | CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-CMVp-GFP
pFUGW
|
Add to Cart
|
| pAWp7-3 | UBCp-RFP-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-CMVp-GFP-[miR-132]
pFUGW
|
Add to Cart
|
| pAWp7-5 | UBCp-RFP-CMVp-GFP-[miR-128 + miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]
pFUGW
|
Add to Cart
|
| pAWp7-7 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP
pFUGW
|
Add to Cart
|
| pAWp7-9 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP
pFUGW
|
Add to Cart
|
| pAWp7-10 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]
pFUGW
|
Add to Cart
|
| pAWp7-12 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]
pFUGW
|
Add to Cart
|
| pAWp7-13 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]
pFUGW
|
Add to Cart
|
| pAWp7-15 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]
pFUGW
|
Add to Cart
|
| pAWp7-16 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]
pFUGW
|
Add to Cart
|
| pAWp7-18 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]
pFUGW
|
Add to Cart
|
| pAWp7-19 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]
pFUGW
|
Add to Cart
|
| pAWp7-20 | UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]
pFUGW
|
Add to Cart
|
| pAWSV-22 | [miR-24-2/27a/23a cluster] | | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
pBT264-[miR-24-2/27a/23a cluster]
pBT264
|
Add to Cart
|
| pLux76-LasI | LasI (Other) | Synthetic Biology | pLux76 | |
Haseloff
| Orthogonal intercellular signaling for programmed spatial behavior.
Mol Syst Biol. 2016 Jan 25;12(1):849.
|
HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL
pSB6A1
|
Add to Cart
|
| pLas81-LuxI | LuxI (Other) | Synthetic Biology | pLas81 | |
Haseloff
| Orthogonal intercellular signaling for programmed spatial behavior.
Mol Syst Biol. 2016 Jan 25;12(1):849.
|
HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL
pSB6A1
|
Add to Cart
|
| pTriEx-mCherry-LINXa3 | LINXa3 (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | Chicken B-Actin | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
Light-induced nuclear export switch for microscopy in mammalian cells.
pTriEx
|
Add to Cart
|
| pTriEx-mCherry-LINXb3 | LINXb3 (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
Light-induced nuclear export switch for microscopy in mammalian cells.
pTriEx
|
Add to Cart
|
| pTriEx-mCherry-LINXa3-nano | LINXa3-nano (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.
pTriEx
|
Add to Cart
|
| pJKR-H-benM | muconate biosensing apparatus | | | |
Church
| Genetically encoded sensors enable real-time observation of metabolite production.
Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
|
Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.
pUC
|
Add to Cart
|
| Flamindo2 | Flamindo2 | Mammalian Expression | CMV | |
Kitaguchi
| Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.
PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
|
Expresses biosensor for cAMP in mammalian cells
pcDNA3.1 (-)
|
Add to Cart
|
| nls-Flamindo2 | nls-Flamindo2 | Mammalian Expression | CMV | |
Kitaguchi
| Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.
PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
|
Expresses biosensor for cAMP in mammalian cells with nuclear localization signal
pcDNA3.1 (-)
|
Add to Cart
|
| mRFP-Lact-C2 | Lactadherin-C2 (Bos taurus) | Mammalian Expression | CMV | mRFP |
Grinstein
| Membrane phosphatidylserine regulates surface charge and protein localization.
Science. 2008 Jan 11. 319(5860):210-3.
|
Fluorescent biosensor for phosphatidylserine.
mRFP-C1
MFGE8
|
Add to Cart
|
| pZ8-P_dCas9 | dcas9 | Bacterial Expression, CRISPR, Synthetic Biology | Propionate inducible promoter (prp) | |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
pZ8-Prp
|
Add to Cart
|
| pZ8-Prp | Propionate inducible promoter | Bacterial Expression, Synthetic Biology | prp | |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
pZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR
pZ8-1
|
Add to Cart
|
| ZIKV_Sensor_27B_LacZ | ZIKV_sensor_27B_LacZ (Synthetic) | Bacterial Expression | T7 | LacZ |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Toehold switch sensor to detect Zika Virus
pCOLA Duet
Tag
/ Fusion Protein- LacZ (C terminal on insert)
|
Add to Cart
|
| ZIKV_Sensor_32B_LacZ | ZIKV_sensor_32B_LacZ (Synthetic) | Bacterial Expression | T7 | LacZ |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Toehold switch sensor to detect Zika Virus
pCOLA Duet
Tag
/ Fusion Protein- LacZ (C terminal on insert)
|
Add to Cart
|
| ZIKV_Trigger_27B | ZIKV_Trigger_27B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence
pET-15b
|
Add to Cart
|
| ZIKV_Trigger_32B | ZIKV_Trigger_32B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence
pET-15b
|
Add to Cart
|
| ZIKV_NASBA_27B | ZIKV_NASBA_27B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites
pET-15b
|
Add to Cart
|
| ZIKV_NASBA_32B | ZIKV_NASBA_32B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites
pET-15b
|
Add to Cart
|
| pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160) | Renilla / P19 (Other) | Plant Expression, Luciferase, Synthetic Biology | 35S | |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
Module for the expression of the Renilla Luciferase with the silencing suppressor P19
pDGB1alpha2
|
Add to Cart
|
| pET22b_PstS_1 | pstS (A197C) (Other) | Bacterial Expression | T7 | |
Webb
| Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
|
Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
pET22b
|
Add to Cart
|
| pET22b_PstS_2 | pstS (A17C,A197C) (Other) | Bacterial Expression | T7 | |
Webb
| Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
|
Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
pET22b
|
Add to Cart
|
| pHis17_ParM _1 | parM (K33A I27C T174A T175N C287A) (Other) | Bacterial Expression | unknown | His6 |
Webb
| A biosensor for fluorescent determination of ADP with high time resolution.
J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29.
|
Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
pHis17
Tag
/ Fusion Protein- His6 (C terminal on backbone)
|
Add to Cart
|
| pET22b_EcSSB_1 | ssb (G26C) (Other) | Bacterial Expression | T7 | |
Webb
| Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.
Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3.
|
Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
pET22b
|
Add to Cart
|
| pET22b_EcSSB_2 | ssb (W88C) (Other) | Bacterial Expression | T7 | |
Webb
| Visualizing helicases unwinding DNA at the single molecule level.
Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28.
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Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
pET22b
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| pTEV5_ RpMatB_1 | matB (His6 C106A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase.
ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21.
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Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
pTEV5
Tag
/ Fusion Protein- His6 (N terminal on backbone)
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| pTEV5_ RpMatB_2 | matB (His6 C106A S170A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
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Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
pTEV5
Tag
/ Fusion Protein- His6 (N terminal on backbone)
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| pTEV5_ RpMatB_3 | matB (His6 C106A T167A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
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Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding
pTEV5
Tag
/ Fusion Protein- His6 (N terminal on backbone)
|
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| pTEV5_ RpMatB_4 | matB (His6 C106A R286C T303A Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
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Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding
pTEV5
Tag
/ Fusion Protein- His6 (N terminal on backbone)
|
Add to Cart
|
Synthetic Biology: Sensing and Signaling