| pAL149 | pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | CMV | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL175 | p!SV40-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL188 | p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL189 | p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL190 | p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL197 | p!SV40-PakGBD-mCherry (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL198 | p!SV40-mCherry-WaspGBD (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL204 | pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL113 | pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL101 | pCMV-PIF6APB-mYFP (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pAL130 | pCMV-PIF3NT-mYFP (Mus musculus) | Mammalian Expression, Synthetic Biology | | |
Voigt
| Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Nature. 2009 Sep 13. ():.
|
| pCph8 | pCph8 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Synthetic biology: engineering Escherichia coli to see light.
Nature. 2005 Nov 24. 438(7067):441-2.
|
| pHL32 (in cHNL135) | | Bacterial Expression, Synthetic Biology | | |
Lim
| A multistep epigenetic switch enables the stable inheritance of DNA methylation states.
Nat Genet. 2007 Feb . 39(2):269-75.
|
| pJT103 | PompC-lacZ | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
| pJT104 | Edge detector delta luxI | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
| pJT105 | Edge detector delta cI | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
| pEDL3 | Edge detector | Bacterial Expression, Synthetic Biology | | |
Voigt
| A synthetic genetic edge detection program.
Cell. 2009 Jun 26. 137(7):1272-81.
|
| pJT106b | red light inverter | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
| pJT106b3 | red light inverter with lacZ output | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
| pJT116 | cca cluster from Synechocystis PCC6803 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
| pJT118 | pJT118 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
| pJT122 | pJT122 | Bacterial Expression, Synthetic Biology | | |
Voigt
| Multichromatic control of gene expression in Escherichia coli.
J Mol Biol. 2011 Jan 14. 405(2):315-24.
|
| pET21b-LOV-ipaA | LOV-ipaA (Synthetic) | Bacterial Expression | T7 | 6xHis |
Kuhlman
| Designing photoswitchable peptides using the AsLOV2 domain.
Chem Biol. 2012 Apr 20;19(4):507-17.
|
| pET21b-LOV-ipaA L514K L531E | LOV-ipaA L514K L531E (Synthetic) | Bacterial Expression | T7 | 6xHis |
Kuhlman
| Designing photoswitchable peptides using the AsLOV2 domain.
Chem Biol. 2012 Apr 20;19(4):507-17.
|
| pZE-PalkB-GFP | GFP, PalkB | Bacterial Expression, Synthetic Biology | PalkB, PalkB | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
| pACYC-PLlambda-alkS(WT) | AlkS(WT) | Bacterial Expression, Synthetic Biology | PLlambda | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
| pACYC-PLlambda-alkSm4-3 | AlkSm4-3 (Other) | Synthetic Biology | PLlambda | |
Alper
| Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes.
J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
|
| pCS-PesaRlux | PesaR (Other) | Bacterial Expression, Synthetic Biology | PesaR | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pCS-PluxIlux | PluxI (Other) | Bacterial Expression, Synthetic Biology | PluxI | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR | Plac-esaR-terminator | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR-I70V/D91G | esaR-I70V/D91G | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR-V220A | esaR-V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR-D91G | esaR-D91G | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR-I70V/D91G/V220A | esaR-I70V/D91G/V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pAC-EsaR-D91G/V220A | esaR-D91G/V220A | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pCS-PesaR-Dlux | PesaR-D | Bacterial Expression, Synthetic Biology | PesaR-D | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pCS-PesaR-Plux | PesaR-P | Bacterial Expression, Synthetic Biology | PesaR-P | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pET-PesaR-Dlux | PesaR-D | Bacterial Expression, Synthetic Biology | PesaR-D | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pET-PesaR-Clux | PesaR-C | Bacterial Expression, Synthetic Biology | PesaR-C | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pET-PesaR-Plux | PesaR-P | Bacterial Expression, Synthetic Biology | PesaR-P | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pAC-EsaR-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pAC-EsaR-I70V-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pAC-EsaR-D91G-EsaI | esaI-term | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pAC-EsaR-I70V | esaR-I70V | Bacterial Expression, Synthetic Biology | Plac | |
Collins
| Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
|
| pET-PesaRlux | PesaR-luxCDABE-term | Bacterial Expression, Synthetic Biology | PesaR | |
Collins
| Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression.
ACS Synth Biol. 2013 Jul 23.
|
| pTD103aiiA | aiiA and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA (TSAANDENYALAA) |
Hasty
| A synchronized quorum of genetic clocks.
Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753.
|
| pTD103luxI_sfGFP | LuxI, sfGFP, and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pTD103aiiA(Cm) | aiiA and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pTD103luxI_sfGFP -R | sfGFP and luxI | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pTD103aiiA(Cm) -R | aiiA | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pZSm45 ndhII | ndhII and LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pZSm45 sodA | sodA LuxR | Bacterial Expression, Synthetic Biology | pLux | ssrA tag (AANDENYALAA) |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pZSm47a luxR RBS | luxR | Bacterial Expression, Synthetic Biology | pArsR | |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pZS47a luxI-laa RBS | LuxI | Bacterial Expression, Synthetic Biology | pArsR | |
Hasty
| A sensing array of radically coupled genetic 'biopixels'.
Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
|
| pHR SFFVp PHAkt Cerulean | PHAkt | Lentiviral, Synthetic Biology | | Cerulean |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
| pHR SFFVp Phy-mCherry-CAAX | PhyB 1-908 (Arabidopsis thaliana) | Lentiviral, Synthetic Biology | | |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
| pHR iSH-YFP-PIF | p85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral, Synthetic Biology | | |
Lim
| Light-based feedback for controlling intracellular signaling dynamics.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
|
| pHR SFFVp BFP-Erk2 | Erk2 (Mus musculus) | Lentiviral | | TagBFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
| pHR SFFVp BFP-PIF-SOS2cat | SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral | | TagBFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
| pHR SFFVp YFP-PIF-SOS2cat | SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana) | Lentiviral | | YFP |
Lim
| Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module.
Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
|
| pP3-GFP | P3 promoter (Other) | Bacterial Expression, Synthetic Biology | P3 | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
| pXylA-agrCA-I | agrCA locus (Other) | Bacterial Expression, Synthetic Biology | PxylA | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
| pXylA-agrCA-IV | agrCA locus (Other) | Bacterial Expression, Synthetic Biology | PxylA | none |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
| pT7-agrBD-I | agrBD locus (Other) | Bacterial Expression, Synthetic Biology | T7-lac | V5 epitope, 6x HIS (not expressed on agrB or agrD) |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
| pT7-agrBD-IV | agrBD locus (with D29Y mutation in agrD) (Other) | Bacterial Expression, Synthetic Biology | T7-lac | V5 epitope, 6x HIS (not expressed on agrB or agrD) |
Collins
| Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling.
Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
|
| tTA_BFP | tTA transcription factor fused to enhanced blue fluorescent protein (Synthetic) | Mammalian Expression | CMV | BFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| pBI-MCS-EYFP | enhanced yellow fluorescent protein (Synthetic) | Mammalian Expression | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| mCherry_LD 0_CS G_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| mCherry_LD 0_TEV | MESA protease chain with mCherry ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| CD4_LD 0_CS G_tTA-BFP | MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| CD4_LD 0_TEV | MESA protease chain with CD4 ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| dTomato_LD 0_CS G_tTA-BFP | MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| dTomato_LD 0_TEV | MESA protease chain with dTomato ectodomain and TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| mCherry_LD 0_CS A_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| mCherry_LD 0_CS M_tTA-BFP | MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| dTomato_LD 0_CS M_tTA-BFP | MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| FRB_LD 0_CS G_tTA-BFP | MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic) | Mammalian Expression, AAV | CMV | EBFP |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| FKBP_LD 0_TEV | MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic) | Mammalian Expression, AAV | CMV | mCherry |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| FKBP_SCF2_LD6_NTEV | MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| FRB_SCF2_LD6_CTEV | MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic) | Mammalian Expression, AAV | CMV | |
Leonard
| Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
ACS Synth Biol. 2014 Mar 11.
|
| pFF145 | Beta recombinase (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF530 | msd_kanR(ON) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF706 | SCRIBE_kanR(ON) (Other) | Synthetic Biology | pDawn | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF714 | SCRIBE_galK(OFF) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF745 | SCRIBE_kanR(ON) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF746 | SCRIBE_galK(ON) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF749 | msd_kanR(ON)_dRT (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF753 | msd(wt) (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF758 | msd(wt)_dRT (Other) | Synthetic Biology | pLacO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF761 | SCRIBE_galK(OFF) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF828 | msd_lacZ(ON) (Other) | Synthetic Biology | pLuxR | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pFF838 | SCRIBE_lacZ(ON) (Other) | Synthetic Biology | pTetO | |
Lu
| Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations.
Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
|
| pJKR-H-cdaR | beta lactamase, sfGFP, CdaR | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-L-cdaR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-H-mphR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-L-mphR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-H-tetR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-L-tetR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-H-araC | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-H-ttgR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-L-ttgR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-H-acuR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-L-acuR | GFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-O-acuR | CFP | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJKR-O-mphR | mCherry | Bacterial Expression | | |
Church
| Synthetic biosensors for precise gene control and real-time monitoring of metabolites.
Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
|
| pJexpress414_OmpA171_R4cons | OmpA_R4cons (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_WT | OmpA (Other) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H1346 | OmpA_H1346 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H1256 | OmpA_H1256 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H2345 | OmpA_H2345 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H3567 | OmpA_H3567 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H2368 | OmpA_H2368 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H1678 | OmpA_H1678 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_WTnoGirdles | OmpA_noGirdles (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pSR59.4 | OmpR (Other), sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
| pSR58.6 | CcaR (Other), sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
| pSR43.6r | CcaS (Other), Ho1 (Other), PcyA (Other) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
| pSR33.4r | Cph8 (Synthetic), Ho1 (Other), PcyA (Other) | Bacterial Expression, Synthetic Biology | | |
Tabor
| Refactoring and optimization of light-switchable escherichia coli two-component systems.
ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
|
| pJexpress414_OmpA171_H1237 | OmpA_H1237 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H2578 | OmpA_H2578 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H3478 | OmpA_H3478 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H2467 | OmpA_H2467 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H1457 | OmpA_H1457 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H4568 | OmpA_H4568 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H1358 | OmpA_H1358 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_R4cons_G125L | OmpA_R4cons_G125L (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_R4cons_G41A | OmpA_R4cons_G41A (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_R4cons_G99I | OmpA_R4cons_G99I (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_R4cons_P121F | OmpA_R4cons_P121F (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_R4cons_Y129H | OmpA_R4cons_Y129H (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H-3 | OmpA_H-3 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H-46 | OmpA_H-46 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H346 | OmpA_H346 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pJexpress414_OmpA171_H-78 | OmpA_H-78 (Synthetic) | Bacterial Expression, Synthetic Biology | T7 | FlAsH, 6His |
Whitehead
| Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
|
| pSCKbreak12 | CisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| pSCKtheoRaj11 | TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| pSCKtheoRaj12-olsa | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| pSCKtheoRaj12-o11 | TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic) | Bacterial Expression, Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| pSCKtheoRaj12 | TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic) | Synthetic Biology | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| pSCKtheoRaj12m | pSCKtheoRaj12m (Synthetic) | Synthetic Biology ; pSC101 origin of replication | | |
Jaramillo
| Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
|
| Dazl-CPG-TV | 5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
| GAPDH-CPG-TV | 5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
| SOX2se-CPG-TV | 5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic) | Mouse Targeting, Synthetic Biology | | |
Jaenisch
| Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution.
Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
|
| pWKS1583 | amyEopt (Synthetic) | Bacterial Expression, Synthetic Biology | Ptet | |
Smits
| The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synth Biol. 2016 Jun 23.
|
| pAP24 | sLucopt (Synthetic) | Bacterial Expression, Synthetic Biology | Ptet | |
Smits
| The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile.
ACS Synth Biol. 2016 Jun 23.
|
| pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632) | sfGFP-ssrA | | | |
Murray
| Rapid cell-free forward engineering of novel genetic ring oscillators.
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
|
| pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588) | mCherry-ssrA | | | |
Murray
| Rapid cell-free forward engineering of novel genetic ring oscillators.
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
|
| pCasper4-g-osk-TRICK | oskar (Drosophila melanogaster) | Insect Expression ; P element-mediated germline transformation | osk | 6xMS2, 12xPP7 |
Ephrussi
| Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.
Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
|
| pAS1NB c Rosella I | Rosella (Synthetic) | Yeast Expression | PGK1 | |
Prescott
| Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast.
Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
|
| pAS1NB m Rosella I | Rosella (Synthetic) | Yeast Expression | PGK1 | yeast mitochondrial targeting sequence of citrate synthase |
Prescott
| Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast.
Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
|
| pGM55 | GM-CSF minimal promoter (-55 to +28) (Homo sapiens) | Mammalian Expression, Luciferase | GM-CSF minimal | luciferase |
Cockerill
| Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication.
Plasmid. 2000 Sep;44(2):173-82.
|
| CY026 | RhlR, CinR | Bacterial Expression, Synthetic Biology | | |
Bennett
| SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium.
Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
|
| CY027 | | Bacterial Expression, Synthetic Biology | | |
Bennett
| SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium.
Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
|
| pAWp7 | UBCp-RFP-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp11 | | Lentiviral | CMVp | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp12 | CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-3 | UBCp-RFP-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-5 | UBCp-RFP-CMVp-GFP-[miR-128 + miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-7 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-9 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-10 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-12 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-13 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-15 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-16 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-18 | UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-19 | UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWp7-20 | UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132] | Lentiviral | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pAWSV-22 | [miR-24-2/27a/23a cluster] | | | |
Lu
| Massively parallel high-order combinatorial genetics in human cells.
Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
|
| pLux76-LasI | LasI (Other) | Synthetic Biology | pLux76 | |
Haseloff
| Orthogonal intercellular signaling for programmed spatial behavior.
Mol Syst Biol. 2016 Jan 25;12(1):849.
|
| pLas81-LuxI | LuxI (Other) | Synthetic Biology | pLas81 | |
Haseloff
| Orthogonal intercellular signaling for programmed spatial behavior.
Mol Syst Biol. 2016 Jan 25;12(1):849.
|
| pTriEx-mCherry-LINXa3 | LINXa3 (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | Chicken B-Actin | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
| pTriEx-mCherry-LINXb3 | LINXb3 (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
| pTriEx-mCherry-LINXa3-nano | LINXa3-nano (Synthetic) | Mammalian Expression, Bacterial Expression, Insect Expression | | mCherry |
Kuhlman
| Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
|
| pJKR-H-benM | muconate biosensing apparatus | | | |
Church
| Genetically encoded sensors enable real-time observation of metabolite production.
Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
|
| Flamindo2 | Flamindo2 | Mammalian Expression | CMV | |
Kitaguchi
| Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.
PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
|
| nls-Flamindo2 | nls-Flamindo2 | Mammalian Expression | CMV | |
Kitaguchi
| Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.
PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
|
| mRFP-Lact-C2 | Lactadherin-C2 (Bos taurus) | Mammalian Expression | CMV | mRFP |
Grinstein
| Membrane phosphatidylserine regulates surface charge and protein localization.
Science. 2008 Jan 11. 319(5860):210-3.
|
| pZ8-P_dCas9 | dcas9 | Bacterial Expression, CRISPR, Synthetic Biology | Propionate inducible promoter (prp) | |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| pZ8-Prp | Propionate inducible promoter | Bacterial Expression, Synthetic Biology | prp | |
Lu
| Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
ACS Synth Biol. 2016 Feb 16.
|
| ZIKV_Sensor_27B_LacZ | ZIKV_sensor_27B_LacZ (Synthetic) | Bacterial Expression | T7 | LacZ |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| ZIKV_Sensor_32B_LacZ | ZIKV_sensor_32B_LacZ (Synthetic) | Bacterial Expression | T7 | LacZ |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| ZIKV_Trigger_27B | ZIKV_Trigger_27B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| ZIKV_Trigger_32B | ZIKV_Trigger_32B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| ZIKV_NASBA_27B | ZIKV_NASBA_27B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| ZIKV_NASBA_32B | ZIKV_NASBA_32B (Synthetic) | Bacterial Expression | T7 | |
Collins
| Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
|
| pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160) | Renilla / P19 (Other) | Plant Expression, Luciferase, Synthetic Biology | 35S | |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
| pET22b_PstS_1 | pstS (A197C) (Other) | Bacterial Expression | T7 | |
Webb
| Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
|
| pET22b_PstS_2 | pstS (A17C,A197C) (Other) | Bacterial Expression | T7 | |
Webb
| Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range.
Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
|
| pHis17_ParM _1 | parM (K33A I27C T174A T175N C287A) (Other) | Bacterial Expression | unknown | His6 |
Webb
| A biosensor for fluorescent determination of ADP with high time resolution.
J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29.
|
| pET22b_EcSSB_1 | ssb (G26C) (Other) | Bacterial Expression | T7 | |
Webb
| Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.
Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3.
|
| pET22b_EcSSB_2 | ssb (W88C) (Other) | Bacterial Expression | T7 | |
Webb
| Visualizing helicases unwinding DNA at the single molecule level.
Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28.
|
| pTEV5_ RpMatB_1 | matB (His6 C106A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase.
ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21.
|
| pTEV5_ RpMatB_2 | matB (His6 C106A S170A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
|
| pTEV5_ RpMatB_3 | matB (His6 C106A T167A R286C Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
|
| pTEV5_ RpMatB_4 | matB (His6 C106A R286C T303A Q457C K488A) (Other) | Bacterial Expression | T7 | His6 |
Webb
| Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
|