Synthetic Biology: Sensing and Signaling

Browse Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing.

Sensing and Signaling Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

	Plasmid	Gene/Insert	Vector Type	Promoter	Tags	PI	Publication
	pAL149	pCMV-PhyB(1-908)-10aaLinker-mCherry-Kras4BCT (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology	CMV		Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL175	p!SV40-mYFP-PIF6APB (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL188	p!SV40-TiamDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL189	p!SV40-IntersectinDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL190	p!SV40-TimDHPH-20aaLinker-mYFP-PIF6APB (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL197	p!SV40-PakGBD-mCherry (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL198	p!SV40-mCherry-WaspGBD (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL204	pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (Arabidopsis thaliana)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL113	pCMV-PhyBNT(Y276H)-10aaLinker-mCFP-HrasCT (Mus musculus)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL101	pCMV-PIF6APB-mYFP (Mus musculus)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pAL130	pCMV-PIF3NT-mYFP (Mus musculus)	Mammalian Expression, Synthetic Biology			Voigt	Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Sep 13. ():.
	pCph8	pCph8	Bacterial Expression, Synthetic Biology			Voigt	Synthetic biology: engineering Escherichia coli to see light. Nature. 2005 Nov 24. 438(7067):441-2.
	pHL32 (in cHNL135)		Bacterial Expression, Synthetic Biology			Lim	A multistep epigenetic switch enables the stable inheritance of DNA methylation states. Nat Genet. 2007 Feb . 39(2):269-75.
	pJT103	PompC-lacZ	Bacterial Expression, Synthetic Biology			Voigt	A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
	pJT104	Edge detector delta luxI	Bacterial Expression, Synthetic Biology			Voigt	A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
	pJT105	Edge detector delta cI	Bacterial Expression, Synthetic Biology			Voigt	A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
	pEDL3	Edge detector	Bacterial Expression, Synthetic Biology			Voigt	A synthetic genetic edge detection program. Cell. 2009 Jun 26. 137(7):1272-81.
	pJT106b	red light inverter	Bacterial Expression, Synthetic Biology			Voigt	Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
	pJT106b3	red light inverter with lacZ output	Bacterial Expression, Synthetic Biology			Voigt	Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
	pJT116	cca cluster from Synechocystis PCC6803	Bacterial Expression, Synthetic Biology			Voigt	Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
	pJT118	pJT118	Bacterial Expression, Synthetic Biology			Voigt	Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
	pJT122	pJT122	Bacterial Expression, Synthetic Biology			Voigt	Multichromatic control of gene expression in Escherichia coli. J Mol Biol. 2011 Jan 14. 405(2):315-24.
	pET21b-LOV-ipaA	LOV-ipaA (Synthetic)	Bacterial Expression	T7	6xHis	Kuhlman	Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
	pET21b-LOV-ipaA L514K L531E	LOV-ipaA L514K L531E (Synthetic)	Bacterial Expression	T7	6xHis	Kuhlman	Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012 Apr 20;19(4):507-17.
	pZE-PalkB-GFP	GFP, PalkB	Bacterial Expression, Synthetic Biology	PalkB, PalkB		Alper	Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
	pACYC-PLlambda-alkS(WT)	AlkS(WT)	Bacterial Expression, Synthetic Biology	PLlambda		Alper	Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
	pACYC-PLlambda-alkSm4-3	AlkSm4-3 (Other)	Synthetic Biology	PLlambda		Alper	Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.
	pCS-PesaRlux	PesaR (Other)	Bacterial Expression, Synthetic Biology	PesaR		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pCS-PluxIlux	PluxI (Other)	Bacterial Expression, Synthetic Biology	PluxI		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR	Plac-esaR-terminator	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR-I70V/D91G	esaR-I70V/D91G	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR-V220A	esaR-V220A	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR-D91G	esaR-D91G	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR-I70V/D91G/V220A	esaR-I70V/D91G/V220A	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pAC-EsaR-D91G/V220A	esaR-D91G/V220A	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pCS-PesaR-Dlux	PesaR-D	Bacterial Expression, Synthetic Biology	PesaR-D		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pCS-PesaR-Plux	PesaR-P	Bacterial Expression, Synthetic Biology	PesaR-P		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pET-PesaR-Dlux	PesaR-D	Bacterial Expression, Synthetic Biology	PesaR-D		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pET-PesaR-Clux	PesaR-C	Bacterial Expression, Synthetic Biology	PesaR-C		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pET-PesaR-Plux	PesaR-P	Bacterial Expression, Synthetic Biology	PesaR-P		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pAC-EsaR-EsaI	esaI-term	Bacterial Expression, Synthetic Biology	Plac		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pAC-EsaR-I70V-EsaI	esaI-term	Bacterial Expression, Synthetic Biology	Plac		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pAC-EsaR-D91G-EsaI	esaI-term	Bacterial Expression, Synthetic Biology	Plac		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pAC-EsaR-I70V	esaR-I70V	Bacterial Expression, Synthetic Biology	Plac		Collins	Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
	pET-PesaRlux	PesaR-luxCDABE-term	Bacterial Expression, Synthetic Biology	PesaR		Collins	Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent Gene Expression. ACS Synth Biol. 2013 Jul 23.
	pTD103aiiA	aiiA and LuxR	Bacterial Expression, Synthetic Biology	pLux	ssrA (TSAANDENYALAA)	Hasty	A synchronized quorum of genetic clocks. Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753.
	pTD103luxI_sfGFP	LuxI, sfGFP, and LuxR	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pTD103aiiA(Cm)	aiiA and LuxR	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pTD103luxI_sfGFP -R	sfGFP and luxI	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pTD103aiiA(Cm) -R	aiiA	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pZSm45 ndhII	ndhII and LuxR	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pZSm45 sodA	sodA LuxR	Bacterial Expression, Synthetic Biology	pLux	ssrA tag (AANDENYALAA)	Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pZSm47a luxR RBS	luxR	Bacterial Expression, Synthetic Biology	pArsR		Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pZS47a luxI-laa RBS	LuxI	Bacterial Expression, Synthetic Biology	pArsR		Hasty	A sensing array of radically coupled genetic 'biopixels'. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
	pHR SFFVp PHAkt Cerulean	PHAkt	Lentiviral, Synthetic Biology		Cerulean	Lim	Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
	pHR SFFVp Phy-mCherry-CAAX	PhyB 1-908 (Arabidopsis thaliana)	Lentiviral, Synthetic Biology			Lim	Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
	pHR iSH-YFP-PIF	p85 iSH2 (Mus musculus), PIF6 1-100 (Arabidopsis thaliana)	Lentiviral, Synthetic Biology			Lim	Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
	pHR SFFVp BFP-Erk2	Erk2 (Mus musculus)	Lentiviral		TagBFP	Lim	Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
	pHR SFFVp BFP-PIF-SOS2cat	SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)	Lentiviral		TagBFP	Lim	Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
	pHR SFFVp YFP-PIF-SOS2cat	SOS2cat (Homo sapiens), PIF6 1-100 (Arabidopsis thaliana)	Lentiviral		YFP	Lim	Using optogenetics to interrogate the dynamic control of signal transmission by the ras/erk module. Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
	pP3-GFP	P3 promoter (Other)	Bacterial Expression, Synthetic Biology	P3	none	Collins	Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
	pXylA-agrCA-I	agrCA locus (Other)	Bacterial Expression, Synthetic Biology	PxylA	none	Collins	Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
	pXylA-agrCA-IV	agrCA locus (Other)	Bacterial Expression, Synthetic Biology	PxylA	none	Collins	Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
	pT7-agrBD-I	agrBD locus (Other)	Bacterial Expression, Synthetic Biology	T7-lac	V5 epitope, 6x HIS (not expressed on agrB or agrD)	Collins	Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
	pT7-agrBD-IV	agrBD locus (with D29Y mutation in agrD) (Other)	Bacterial Expression, Synthetic Biology	T7-lac	V5 epitope, 6x HIS (not expressed on agrB or agrD)	Collins	Peptide-based communication system enables Escherichia coli to Bacillus megaterium interspecies signaling. Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5.
	tTA_BFP	tTA transcription factor fused to enhanced blue fluorescent protein (Synthetic)	Mammalian Expression	CMV	BFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	pBI-MCS-EYFP	enhanced yellow fluorescent protein (Synthetic)	Mammalian Expression	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	mCherry_LD 0_CS G_tTA-BFP	MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	mCherry_LD 0_TEV	MESA protease chain with mCherry ectodomain and TEV protease (Synthetic)	Mammalian Expression, AAV	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	CD4_LD 0_CS G_tTA-BFP	MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	CD4_LD 0_TEV	MESA protease chain with CD4 ectodomain and TEV protease (Synthetic)	Mammalian Expression, AAV	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	dTomato_LD 0_CS G_tTA-BFP	MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	dTomato_LD 0_TEV	MESA protease chain with dTomato ectodomain and TEV protease (Synthetic)	Mammalian Expression, AAV	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	mCherry_LD 0_CS A_tTA-BFP	MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1') (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	mCherry_LD 0_CS M_tTA-BFP	MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	dTomato_LD 0_CS M_tTA-BFP	MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1') (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	FRB_LD 0_CS G_tTA-BFP	MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence (Synthetic)	Mammalian Expression, AAV	CMV	EBFP	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	FKBP_LD 0_TEV	MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion (Synthetic)	Mammalian Expression, AAV	CMV	mCherry	Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	FKBP_SCF2_LD6_NTEV	MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease (Synthetic)	Mammalian Expression, AAV	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	FRB_SCF2_LD6_CTEV	MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease (Synthetic)	Mammalian Expression, AAV	CMV		Leonard	Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. ACS Synth Biol. 2014 Mar 11.
	pFF145	Beta recombinase (Other)	Synthetic Biology	pTetO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF530	msd_kanR(ON) (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF706	SCRIBE_kanR(ON) (Other)	Synthetic Biology	pDawn		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF714	SCRIBE_galK(OFF) (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF745	SCRIBE_kanR(ON) (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF746	SCRIBE_galK(ON) (Other)	Synthetic Biology	pTetO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF749	msd_kanR(ON)_dRT (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF753	msd(wt) (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF758	msd(wt)_dRT (Other)	Synthetic Biology	pLacO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF761	SCRIBE_galK(OFF) (Other)	Synthetic Biology	pTetO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF828	msd_lacZ(ON) (Other)	Synthetic Biology	pLuxR		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pFF838	SCRIBE_lacZ(ON) (Other)	Synthetic Biology	pTetO		Lu	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Science. 2014 Nov 14;346(6211):1256272. doi: 10.1126/science.1256272.
	pJKR-H-cdaR	beta lactamase, sfGFP, CdaR	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-L-cdaR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-H-mphR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-L-mphR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-H-tetR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-L-tetR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-H-araC	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-H-ttgR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-L-ttgR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-H-acuR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-L-acuR	GFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-O-acuR	CFP	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJKR-O-mphR	mCherry	Bacterial Expression			Church	Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
	pJexpress414_OmpA171_R4cons	OmpA_R4cons (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_WT	OmpA (Other)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H1346	OmpA_H1346 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H1256	OmpA_H1256 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H2345	OmpA_H2345 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H3567	OmpA_H3567 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H2368	OmpA_H2368 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H1678	OmpA_H1678 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_WTnoGirdles	OmpA_noGirdles (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pSR59.4	OmpR (Other), sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Tabor	Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
	pSR58.6	CcaR (Other), sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Tabor	Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
	pSR43.6r	CcaS (Other), Ho1 (Other), PcyA (Other)	Bacterial Expression, Synthetic Biology			Tabor	Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
	pSR33.4r	Cph8 (Synthetic), Ho1 (Other), PcyA (Other)	Bacterial Expression, Synthetic Biology			Tabor	Refactoring and optimization of light-switchable escherichia coli two-component systems. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11.
	pJexpress414_OmpA171_H1237	OmpA_H1237 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H2578	OmpA_H2578 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H3478	OmpA_H3478 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H2467	OmpA_H2467 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H1457	OmpA_H1457 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H4568	OmpA_H4568 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H1358	OmpA_H1358 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_R4cons_G125L	OmpA_R4cons_G125L (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_R4cons_G41A	OmpA_R4cons_G41A (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_R4cons_G99I	OmpA_R4cons_G99I (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_R4cons_P121F	OmpA_R4cons_P121F (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_R4cons_Y129H	OmpA_R4cons_Y129H (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H-3	OmpA_H-3 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H-46	OmpA_H-46 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H346	OmpA_H346 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pJexpress414_OmpA171_H-78	OmpA_H-78 (Synthetic)	Bacterial Expression, Synthetic Biology	T7	FlAsH, 6His	Whitehead	Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
	pSCKbreak12	CisRaj12-sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	pSCKtheoRaj11	TheoHHAzRaj11,cisRaj11,sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	pSCKtheoRaj12-olsa	TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	pSCKtheoRaj12-o11	TheoHHAzRaj12,cisRaj11,sfGFP (Synthetic)	Bacterial Expression, Synthetic Biology			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	pSCKtheoRaj12	TheoHHAzRaj12,cisRaj12,sfGFP (Synthetic)	Synthetic Biology			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	pSCKtheoRaj12m	pSCKtheoRaj12m (Synthetic)	Synthetic Biology ; pSC101 origin of replication			Jaramillo	Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 10.1093/nar/gkv287. Epub 2015 Apr 27.
	Dazl-CPG-TV	5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL (Synthetic)	Mouse Targeting, Synthetic Biology			Jaenisch	Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
	GAPDH-CPG-TV	5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh (Synthetic)	Mouse Targeting, Synthetic Biology			Jaenisch	Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
	SOX2se-CPG-TV	5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se (Synthetic)	Mouse Targeting, Synthetic Biology			Jaenisch	Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
	pWKS1583	amyEopt (Synthetic)	Bacterial Expression, Synthetic Biology	Ptet		Smits	The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
	pAP24	sLucopt (Synthetic)	Bacterial Expression, Synthetic Biology	Ptet		Smits	The Signal Sequence of the Abundant Extracellular Metalloprotease PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in Clostridium difficile. ACS Synth Biol. 2016 Jun 23.
	pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)	sfGFP-ssrA				Murray	Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
	pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)	mCherry-ssrA				Murray	Rapid cell-free forward engineering of novel genetic ring oscillators. Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
	pCasper4-g-osk-TRICK	oskar (Drosophila melanogaster)	Insect Expression ; P element-mediated germline transformation	osk	6xMS2, 12xPP7	Ephrussi	Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
	pAS1NB c Rosella I	Rosella (Synthetic)	Yeast Expression	PGK1		Prescott	Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
	pAS1NB m Rosella I	Rosella (Synthetic)	Yeast Expression	PGK1	yeast mitochondrial targeting sequence of citrate synthase	Prescott	Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
	pGM55	GM-CSF minimal promoter (-55 to +28) (Homo sapiens)	Mammalian Expression, Luciferase	GM-CSF minimal	luciferase	Cockerill	Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Plasmid. 2000 Sep;44(2):173-82.
	CY026	RhlR, CinR	Bacterial Expression, Synthetic Biology			Bennett	SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
	CY027		Bacterial Expression, Synthetic Biology			Bennett	SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic microbial consortium. Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
	pAWp7	UBCp-RFP-CMVp-GFP	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp11		Lentiviral	CMVp		Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp12	CMVp-GFP	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-3	UBCp-RFP-CMVp-GFP-[miR-132]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-5	UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-7	UBCp-RFP-[miR-124 sensor]-CMVp-GFP	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-9	UBCp-RFP-[miR-132 sensor]-CMVp-GFP	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-10	UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-12	UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-13	UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-15	UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-16	UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-18	UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-19	UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124 + miR-128]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWp7-20	UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128 + miR-132]	Lentiviral			Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pAWSV-22	[miR-24-2/27a/23a cluster]				Lu	Massively parallel high-order combinatorial genetics in human cells. Nat Biotechnol. 2015 Sep;33(9):952-61. doi: 10.1038/nbt.3326. Epub 2015 Aug 17.
	pLux76-LasI	LasI (Other)	Synthetic Biology	pLux76		Haseloff	Orthogonal intercellular signaling for programmed spatial behavior. Mol Syst Biol. 2016 Jan 25;12(1):849.
	pLas81-LuxI	LuxI (Other)	Synthetic Biology	pLas81		Haseloff	Orthogonal intercellular signaling for programmed spatial behavior. Mol Syst Biol. 2016 Jan 25;12(1):849.
	pTriEx-mCherry-LINXa3	LINXa3 (Synthetic)	Mammalian Expression, Bacterial Expression, Insect Expression	Chicken B-Actin	mCherry	Kuhlman	Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
	pTriEx-mCherry-LINXb3	LINXb3 (Synthetic)	Mammalian Expression, Bacterial Expression, Insect Expression		mCherry	Kuhlman	Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
	pTriEx-mCherry-LINXa3-nano	LINXa3-nano (Synthetic)	Mammalian Expression, Bacterial Expression, Insect Expression		mCherry	Kuhlman	Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
	pJKR-H-benM	muconate biosensing apparatus				Church	Genetically encoded sensors enable real-time observation of metabolite production. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375.
	Flamindo2	Flamindo2	Mammalian Expression	CMV		Kitaguchi	Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
	nls-Flamindo2	nls-Flamindo2	Mammalian Expression	CMV		Kitaguchi	Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. doi: 10.1371/journal.pone.0100252. eCollection 2014.
	mRFP-Lact-C2	Lactadherin-C2 (Bos taurus)	Mammalian Expression	CMV	mRFP	Grinstein	Membrane phosphatidylserine regulates surface charge and protein localization. Science. 2008 Jan 11. 319(5860):210-3.
	pZ8-P_dCas9	dcas9	Bacterial Expression, CRISPR, Synthetic Biology	Propionate inducible promoter (prp)		Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pZ8-Prp	Propionate inducible promoter	Bacterial Expression, Synthetic Biology	prp		Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	ZIKV_Sensor_27B_LacZ	ZIKV_sensor_27B_LacZ (Synthetic)	Bacterial Expression	T7	LacZ	Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	ZIKV_Sensor_32B_LacZ	ZIKV_sensor_32B_LacZ (Synthetic)	Bacterial Expression	T7	LacZ	Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	ZIKV_Trigger_27B	ZIKV_Trigger_27B (Synthetic)	Bacterial Expression	T7		Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	ZIKV_Trigger_32B	ZIKV_Trigger_32B (Synthetic)	Bacterial Expression	T7		Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	ZIKV_NASBA_27B	ZIKV_NASBA_27B (Synthetic)	Bacterial Expression	T7		Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	ZIKV_NASBA_32B	ZIKV_NASBA_32B (Synthetic)	Bacterial Expression	T7		Collins	Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016 May 6. pii: S0092-8674(16)30505-0. doi: 10.1016/j.cell.2016.04.059.
	pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)	Renilla / P19 (Other)	Plant Expression, Luciferase, Synthetic Biology	35S		Orzaez	A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
	pET22b_PstS_1	pstS (A197C) (Other)	Bacterial Expression	T7		Webb	Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
	pET22b_PstS_2	pstS (A17C,A197C) (Other)	Bacterial Expression	T7		Webb	Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range. Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
	pHis17_ParM _1	parM (K33A I27C T174A T175N C287A) (Other)	Bacterial Expression	unknown	His6	Webb	A biosensor for fluorescent determination of ADP with high time resolution. J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 10.1074/jbc.M109.047118. Epub 2009 Sep 29.
	pET22b_EcSSB_1	ssb (G26C) (Other)	Bacterial Expression	T7		Webb	Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity. Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. Epub 2008 Jul 3.
	pET22b_EcSSB_2	ssb (W88C) (Other)	Bacterial Expression	T7		Webb	Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res. 2010 Jul;38(13):4448-57. doi: 10.1093/nar/gkq173. Epub 2010 Mar 28.
	pTEV5_ RpMatB_1	matB (His6 C106A R286C Q457C K488A) (Other)	Bacterial Expression	T7	His6	Webb	A Fluorescent, Reagentless Biosensor for ATP, Based on Malonyl-Coenzyme A Synthetase. ACS Chem Biol. 2015 Nov 20;10(11):2650-7. doi: 10.1021/acschembio.5b00346. Epub 2015 Sep 21.
	pTEV5_ RpMatB_2	matB (His6 C106A S170A R286C Q457C K488A) (Other)	Bacterial Expression	T7	His6	Webb	Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
	pTEV5_ RpMatB_3	matB (His6 C106A T167A R286C Q457C K488A) (Other)	Bacterial Expression	T7	His6	Webb	Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
	pTEV5_ RpMatB_4	matB (His6 C106A R286C T303A Q457C K488A) (Other)	Bacterial Expression	T7	His6	Webb	Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase. PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.