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(Plasmid #101167)


Item Catalog # Description Quantity Price (USD)
Plasmid 101167 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    JM Daran
  • Backbone size w/o insert (bp) 10664
  • Total vector size (bp) 10855
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    amdS (acetamidase gene conferring growth on acetamide)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
  • gRNA/shRNA sequence
  • Species
    S. cerevisiae (budding yeast); S. pastorianus
  • GenBank ID
  • Promoter ScTDH3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer CGACAATCTAAGAACTATGCGAGG
  • 3′ sequencing primer CTGGCTATACCTATCCGTCTACG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Spcas9D147Y P411T gene presents on pUDP004, pUDP012 and pUDP044 is derived from pCT (Plasmid #60620) obtained from Addgene under Material Transfer Agreement Instructions (Order 211742- our reference TNW15.216)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUDP012 was a gift from Jean-Marc Daran (Addgene plasmid # 101167 ; ; RRID:Addgene_101167)
  • For your References section:

    CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus. de Vries ARG, de Groot PA, van den Broek M, Daran JG. Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1. 10.1186/s12934-017-0835-1 [pii] PubMed 29207996