PurposeompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||102270||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Low salt LB (5 g/L)
Growth instructionsRequires low salt (5 g/L NaCl or less) in LB. Strain is intrinsically tetracycline-resistant.
Genotype = ΔompA ΔompC ΔompF ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Due to the strain's growth defect and potential for contamination, Addgene recommends screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BL21ΔABCF was a gift from Jack Leo (Addgene plasmid # 102270)
For your References section:A New Strain Collection for Improved Expression of Outer Membrane Proteins. Meuskens I, Michalik M, Chauhan N, Linke D, Leo JC. Front Cell Infect Microbiol. 2017 Nov 7;7:464. doi: 10.3389/fcimb.2017.00464. eCollection 2017. 10.3389/fcimb.2017.00464 PubMed 29164072