Purposebacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104628||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepET His6 TEV LIC cloning vector
Backbone manufacturerScott Gradia (Addgene plasmid # 29653)
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
MutationC450A (dark-locked and light-insensitive mutation)
Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
- Promoter T7
/ Fusion Proteins
- His (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that during Addgene's quality control process 2 mismatches were found in lacI which result in mutations S219F and Q311P.
LOV2 domain was inserted into the intersubunit linker of the caspase, with linker variation N7C-7 and mutation C450A, which is a dark-locked and light-insensitive mutation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LIC1B_Caspase-LOVN7C-7 C450A was a gift from Jim Wells (Addgene plasmid # 104628 ; http://n2t.net/addgene:104628 ; RRID:Addgene_104628)
For your References section:Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Smart AD, Pache RA, Thomsen ND, Kortemme T, Davis GW, Wells JA. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8174-E8183. doi: 10.1073/pnas.1705064114. Epub 2017 Sep 11. 10.1073/pnas.1705064114 PubMed 28893998