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LIC1B_Caspase-LOVN7C-7 C450A
(Plasmid #104628)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104628 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET His6 TEV LIC cloning vector
  • Backbone manufacturer
    Scott Gradia (Addgene plasmid # 29653)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Caspase-3
  • Species
    H. sapiens (human)
  • Mutation
    C450A (dark-locked and light-insensitive mutation)
  • Entrez Gene
    CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • LOV2

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that during Addgene's quality control process 2 mismatches were found in lacI which result in mutations S219F and Q311P.

LOV2 domain was inserted into the intersubunit linker of the caspase, with linker variation N7C-7 and mutation C450A, which is a dark-locked and light-insensitive mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIC1B_Caspase-LOVN7C-7 C450A was a gift from Jim Wells (Addgene plasmid # 104628 ; http://n2t.net/addgene:104628 ; RRID:Addgene_104628)
  • For your References section:

    Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Smart AD, Pache RA, Thomsen ND, Kortemme T, Davis GW, Wells JA. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8174-E8183. doi: 10.1073/pnas.1705064114. Epub 2017 Sep 11. 10.1073/pnas.1705064114 PubMed 28893998