pCR8/GW:PtU6
(Plasmid #104895)

• Purpose: Phaeodactylum U6 promoter
• Depositing Lab: Thomas Mock
• Publication: Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. doi: 10.3389/fmicb.2021.688199. eCollection 2021.

Backbone

• Vector backbone: pCR8/GW/TOPO
• Backbone size w/o insert (bp): 2817

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Phaeodactylum U6 promoter
• Species: Phaeodactylum tricornutum
• Insert Size (bp): 280

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • pCR8 GW:PtU6.gb
• A portion of this plasmid was derived from a plasmid made by: U6 promoter was cloned from gDNA based on Nymark M, Sharma AK, Sparstad T Bones, A, Winge P. A CRISPR/Cas9 system adapted for gene editing in marine algae.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For Golden Gated assembly.

Please visit https://www.biorxiv.org/content/early/2018/10/17/444109 for bioRxiv preprint.