|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85981||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerThermo Fisher
Vector typeCRISPR ; Diatom (T. pseudonana)
Growth in Bacteria
Gene/Insert nameT. pseudonana U6 promoter
- Promoter none
- Cloning method TOPO Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCR8/GW:U6 was a gift from Thomas Mock (Addgene plasmid # 85981 ; http://n2t.net/addgene:85981 ; RRID:Addgene_85981)
For your References section:Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Hopes A, Nekrasov V, Kamoun S and Mock T. Plant Methods 2016, 12:49 10.1186/s13007-016-0148-0