|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85984||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSylvestre Marillonnet (Addgene #48000)
Vector typeGolden Gate Assembly
Selectable markersNourseothricin (Nat)
Growth in Bacteria
Growth instructionsAddgene recommends screening 2-3 colonies via restriction digest before creating a glycerol stock.
Alt namenourseothricin acetyltransferase
- Promoter FCP
- Cloning method Unknown
- 5′ sequencing primer NrsR-F (TGACCACTCTTGACGACACG)
- 3′ sequencing primer pBluescript-SK (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This construct was assembled using Golden Gate Cloning as described in Hopes et al., 2016.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pICH47732:FCP:NAT was a gift from Thomas Mock (Addgene plasmid # 85984 ; http://n2t.net/addgene:85984 ; RRID:Addgene_85984)
For your References section:Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Hopes A, Nekrasov V, Kamoun S and Mock T. Plant Methods 2016, 12:49 10.1186/s13007-016-0148-0