|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85986||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSylvestre Marillonnet (Addgene #48001)
Vector typeCRISPR ; Golden Gate Assembly
Growth in Bacteria
- Promoter FCP
/ Fusion Protein
- SV40 NLS, YFP (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer hSpCas9-R1 (CGCTCGTGCTTCTTATCCTC)
- 3′ sequencing primer EXFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This construct was assembled using Golden Gate Cloning as described in Hopes et al., 2016.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pICH47742:FCP:Cas9YFP was a gift from Thomas Mock (Addgene plasmid # 85986 ; http://n2t.net/addgene:85986 ; RRID:Addgene_85986)
For your References section:Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Hopes A, Nekrasov V, Kamoun S and Mock T. Plant Methods 2016, 12:49 10.1186/s13007-016-0148-0