Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana.
Hopes A, Nekrasov V, Kamoun S, Mock T
Plant Methods. 2016 Nov 24;12:49. doi: 10.1186/s13007-016-0148-0. eCollection 2016. PubMed Article
Hopes A, Nekrasov V, Kamoun S, Mock T
Plant Methods. 2016 Nov 24;12:49. doi: 10.1186/s13007-016-0148-0. eCollection 2016. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85981 | pCR8/GW:U6 | Golden Gate Level 0 cassette encoding the U6 promoter |
| 85982 | pAGM4723:TpCC_Urease | Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs |
| 85984 | pICH47732:FCP:NAT | Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter |
| 85986 | pICH47742:FCP:Cas9YFP | Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter |