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Addgene

pAJM.884
(Plasmid #108536)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108536 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p15A-Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AcuRAM + PAcu-YFP
  • Species
    Synthetic
  • Mutation
    AcuR: E80K R154H L202M

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJM.884 was a gift from Christopher Voigt (Addgene plasmid # 108536 ; http://n2t.net/addgene:108536 ; RRID:Addgene_108536)
  • For your References section:

    Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3. 10.1038/s41589-018-0168-3 PubMed 30478458