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(Plasmid #108571)


Item Catalog # Description Quantity Price (USD)
Plasmid 108571 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    RANKL (receptor activator of nuclear factor kappa-B ligand)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    Tnfsf11 (a.k.a. Ly109l, ODF, OPGL, RANKL, Trance)
  • Promoter Ptac (trp/lac)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GCAAGCTACCTGAAATGCTG
  • 3′ sequencing primer ATACACTCCGCTATCGCTAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T2 RANK-L was a gift from Jean-Claude Scimeca (Addgene plasmid # 108571 ; ; RRID:Addgene_108571)
  • For your References section:

    Differential binding of poly(ADP-Ribose) polymerase-1 and JunD/Fra2 accounts for RANKL-induced Tcirg1 gene expression during osteoclastogenesis. Beranger GE, Momier D, Guigonis JM, Samson M, Carle GF, Scimeca JC. J Bone Miner Res. 2007 Jul;22(7):975-83. doi: 10.1359/jbmr.070406. 10.1359/jbmr.070406 PubMed 17419679