tet-pLKO.neo_shGFP
(Plasmid #110470)

• Purpose: Control, silence GFP gene, doxycycline inducible, neomycin selection
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-9357. doi: 10.1074/jbc.RA119.008180. Epub 2019 Apr 30.

Backbone

• Vector backbone: Tet-pLKO-neo
• Backbone manufacturer: #21916
• Backbone size w/o insert (bp): 10959
• Total vector size (bp): 9142
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• gRNA/shRNA sequence: CAAGCTGACCCTGAAGTTCAT
• Species: Synthetic
• Promoter: H1/TO (RNA PolIII)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGCAGGGATATTCACCATTATCGTTTCAGA

Resource Information

• Supplemental Documents:
  • pD223-tet-pLKO.neo_shGFP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.

Plasmid grows more slowly than standard plasmids.