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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5
(Plasmid #111818)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111818 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEMT-easy
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 8800
  • Vector type
    cloning intermediate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Mef2c
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer M13 F/T7
  • 3′ sequencing primer T2A-r
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Tdtomato
  • Insert Size (bp)
    1400

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T2A-f
  • 3′ sequencing primer P2A-r
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Gata4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BspEI (not destroyed)
  • 5′ sequencing primer P2A-f
  • 3′ sequencing primer E2A-r
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Tbx5
  • Insert Size (bp)
    1500

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer E2A-f
  • 3′ sequencing primer M13R/SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 was a gift from Li Qian (Addgene plasmid # 111818 ; http://n2t.net/addgene:111818 ; RRID:Addgene_111818)
  • For your References section:

    Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J. Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2. 10.1038/s41598-017-02460-2 PubMed 28526819