pmiR-report-hEZH2
(Plasmid
#112543)
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Purpose3'UTR reporter assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepmiR-report
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Backbone manufacturerAmbion
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEZH2
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SpeciesH. sapiens (human)
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Insert Size (bp)225
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Entrez GeneEZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
clone into pmiR-report using SacI/HindIII
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmiR-report-hEZH2 was a gift from Shusheng Wang (Addgene plasmid # 112543 ; http://n2t.net/addgene:112543 ; RRID:Addgene_112543) -
For your References section:
Repression of choroidal neovascularization through actin cytoskeleton pathways by microRNA-24. Zhou Q, Anderson C, Zhang H, Li X, Inglis F, Jayagopal A, Wang S. Mol Ther. 2014 Feb;22(2):378-389. doi: 10.1038/mt.2013.243. Epub 2013 Oct 17. 10.1038/mt.2013.243 PubMed 24297048
