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pOTTC1124 - pAAV MeCP2 SpCas9(D10A)
(Plasmid #112719)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112719 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX551
  • Backbone manufacturer
    Feng Zhang
  • Backbone size w/o insert (bp) 2905
  • Total vector size (bp) 7400
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Alt name
    S. pyogenes Cas9 nuclease
  • Alt name
    Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4200
  • Mutation
    D10A
  • Promoter Mecp2

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AATGGGGTCCGCCTCTTTTCC
  • 3′ sequencing primer cttagctggcctccacctttctcttc
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1124 - pAAV MeCP2 SpCas9(D10A) was a gift from Brandon Harvey (Addgene plasmid # 112719 ; http://n2t.net/addgene:112719 ; RRID:Addgene_112719)
  • For your References section:

    Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. 10.1016/j.neuron.2019.01.035 PubMed 30792150