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pACT2.2gtwy
(Plasmid #11346)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11346 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACT2.2
  • Backbone manufacturer
    see map of pACT2.2 in "author's map"
  • Backbone size w/o insert (bp) 7550
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    This vector is propagated in E. coli strain DB3.1, due to the requirement to circumvent the lethality of the inherent ccdB gene until an insert is recombined into the Gateway cassette.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gateway cassette
  • Tag / Fusion Protein
    • Gal4 Activation Domain (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site SmaI (unknown if destroyed)
  • 5′ sequencing primer Gal4 AD
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Gateway-modified yeast two-hybrid Activation domain vector. Use this vector for Gateway cloning of a cDNA to be used to test a directed yeast two-hybrid interaction. This vector was modified from plasmid pACT2.2 by placing a Gateway acceptor cassette in the SmaI site of pACT2.2. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) Please note that the "author's map" is the map of the original pACT2.2 vector upon which pACT2.2gtwy is derived - it is not a map of pACT2.2gtwy.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2.2gtwy was a gift from Guy Caldwell (Addgene plasmid # 11346 ; http://n2t.net/addgene:11346 ; RRID:Addgene_11346)