pcDNA3.1-CAG-NanoLuc-VChR1-EYFP
(Plasmid #114112)

• Purpose: fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):E358-67. doi: 10.1073/pnas.1510899113. Epub 2016 Jan 5.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 6233
• Total vector size (bp): 8547
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NanoLuc; Volvox channelrhodopsin 1; EYFP
• Alt name: NLuc-VChr1-EYFP
• Species: Synthetic; Oplophorous
• Insert Size (bp): 510
• Promoter: CAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pcDNA3.1 CAG NanoLuc VChR1 EYFP.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry